How To Use Restriction enzyme In A Sentence
-
DNAs were digested with restriction enzymes, separated on agarose gel, and blotted onto a nylon membrane.
-
The binding mechanism of psoralens to a double-strand DNA has been well investigated by chemical and enzymatic methods using restriction enzymes and exonuclease.
-
Illustrated in Figure 7 is the restriction enzyme and genetic map of a commercially available (BD Biosciences Clontech) bacterial plasmid derivative containing the coding sequence for enhanced yellow fluorescent protein fused to the endoplasmic reticulum targeting sequence of calreticulin (a resident protein).
Archive 2005-10-01
-
This, followed by the discovery of restriction enzymes by Werner Arber in the 1960's, has paved the way for many biotechnological procedures such as recombinant DNA technology.
-
If the restriction enzyme activities were inhibited, the DNA fragment would not be cleaved and should be seen in its original size on the gel.
-
When they are inserted into other cells this process appears to protect them against chemicals called restriction enzymes, which defend against viruses.
Times, Sunday Times
-
By restriction enzyme identification, PCR and sequence analysis, the recombinant plasmid of the HA1 was successfully constructed.
-
Control amplifications on samples to show that a constant restriction enzyme site can be cut strengthen confidence in the analysis.
-
Hamilton Smith telling me about the restriction endonuclease he had discovered in Hemophilus influenzae aroused my interest in the possibility of using restriction enzymes to dissect the genomes of DNA tumor viruses.
Daniel Nathans - Autobiography
-
Biogen 1 discloses that the way to do it is to choose the restriction enzymes likely to cleave the Dane particle DNA into the largest fragments.
-
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
-
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
-
Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes.
-
Restriction enzymes and reverse transcriptase are keys to gene engineering.
-
Restriction enzymes and reverse transcriptase are keys to gene engineering.
-
Subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated F' plasmid and that from the autonomous F plasmid.
-
Methods Allele-specific restriction enzyme was used to analyze patient, her mother and brother and 40 healthy volunteers.
-
The Restriction Enzyme Database from NEB.
-
Positive clones of F gene were identified by PCR and restriction enzyme digestion and then sequenced.
-
When they are inserted into other cells this process appears to protect them against chemicals called restriction enzymes, which defend against viruses.
Times, Sunday Times
-
Control amplifications on samples to show that a constant restriction enzyme site can be cut strengthen confidence in the analysis.
-
The restriction digestion system consisted of 10μ L PCR products, 2.0μ L 10× Buffer, 83.350 nkat restriction enzyme and 7.5μ L ddH 2 O.
-
Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.
-
It enables a specific gene to be located on a particular restriction enzyme fragment.
-
To improve the discrimination power of AFLP for peanut cultivars of similar origin, different restriction enzyme combinations and more primer pairs may be needed.
-
Eighteen candidate clones from the genomic library were digested with a series of restriction enzymes, Southern blotted, and hybridized with the same probe.
-
There were restriction enzyme sites of Smaand Sdu in UCP 3 gene of pigs.
-
Double-stranded oligos were multimerized using T4 DNA ligase in the presence of XhoI and SalI restriction enzymes to generate tandem arrays.
-
Methods Isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test, plasmid profiles, restriction enzyme fingerprinting assay and plasmid elimination method.
-
A single band was found for each restriction enzymes and combinations mentioned above.
-
Restriction enzyme map simulated by pDraw32 software is consistent basically with the previous report.
-
The restriction enzyme bound on DNA at the first site binds its second site to a remote DNA sequence and then dissociates from the first one.
-
Abbreviations for restriction enzyme sites: H, Hin dIII.