restriction enzyme

  1. any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
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How To Use restriction enzyme In A Sentence

  • DNAs were digested with restriction enzymes, separated on agarose gel, and blotted onto a nylon membrane.
  • The binding mechanism of psoralens to a double-strand DNA has been well investigated by chemical and enzymatic methods using restriction enzymes and exonuclease.
  • Illustrated in Figure 7 is the restriction enzyme and genetic map of a commercially available (BD Biosciences Clontech) bacterial plasmid derivative containing the coding sequence for enhanced yellow fluorescent protein fused to the endoplasmic reticulum targeting sequence of calreticulin (a resident protein). Archive 2005-10-01
  • This, followed by the discovery of restriction enzymes by Werner Arber in the 1960's, has paved the way for many biotechnological procedures such as recombinant DNA technology.
  • If the restriction enzyme activities were inhibited, the DNA fragment would not be cleaved and should be seen in its original size on the gel.
  • When they are inserted into other cells this process appears to protect them against chemicals called restriction enzymes, which defend against viruses. Times, Sunday Times
  • By restriction enzyme identification, PCR and sequence analysis, the recombinant plasmid of the HA1 was successfully constructed.
  • Control amplifications on samples to show that a constant restriction enzyme site can be cut strengthen confidence in the analysis.
  • Hamilton Smith telling me about the restriction endonuclease he had discovered in Hemophilus influenzae aroused my interest in the possibility of using restriction enzymes to dissect the genomes of DNA tumor viruses. Daniel Nathans - Autobiography
  • Biogen 1 discloses that the way to do it is to choose the restriction enzymes likely to cleave the Dane particle DNA into the largest fragments.
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