How To Use Electrophoresis In A Sentence
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Svedberg's investigations with the ultracentrifuge and Tiselius's electrophoresis studies (see Section 3.10) were instrumental in establishing that protein molecules have a unique size and structure, and this was a prerequisite for Sanger's determination of their amino-acid sequence and the crystallographic work of
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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He added, "The only way to detect the presence of the trait is through a blood test called haemoglobin electrophoresis.
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The proteins in the immunoprecipitates were size fractionated by SDS polyacrylamide gel electrophoresis.
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The albumen zone form with SDS-PAGE electrophoresis were similar in the whole virus vaccines and split-2 vaccine while the albumen zone of the split-1 vaccine could not be seen in 32KD right-and-left.
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The actual test for EPO, what they call electrophoresis, is actually being questioned on a pretty serious level right now.
CNN Transcript Aug 25, 2005
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This revised and expanded edition has two new chapters on capillary electrophoresis and supercritical fluid chromatography.
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Our observation of trapping of small molecules suggests that dielectrophoretic trapping may be applicable to many small molecules, which has not been observed because of the methods used to study dielectrophoresis to date.
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These include the single cell gel electrophoresis assay, the gel electrophoresis assay, the alkaline elution and unwinding assays and the analysis of DNA adducts.
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Although fat ingestion only slightly affects cholesterol levels, both the triglyceride and lipoprotein electrophoresis results are greatly affected.
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All isolates were subjected to molecular typing by pulsed-field gel electrophoresis.
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Possession of the marker is indicated by two ‘bands’ in the electrophoresis lane derived from each well, while one band indicates lack of the marker.
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Employing a process called "electrophoresis," researchers who study DNA typically embed negatively charged DNA into a porous hydrogel.
R&D Mag - News
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Following treatments for 24 hr with DMSO alone (control), Red-Br-nos, LPS, Red-Br-nos and LPS, cells were stained with 1 µg/ml acridine orange for 15 min, washed with PBS, and examined under a Zeiss fluorescence microscope using a 40X objective lens Proteins from THP-1 cells pellets were resolved by polyacrylamide gel-electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore).
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A variety of experimental approaches have been developed to investigate DNA bending and flexibility, including comparative gel electrophoresis, crystallography, electron microscopy, and DNA cyclization.
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Isoelectric focusing (or crossed immunoelectrophoresis and starch gel electrophoresis in early reports) was considered the ‘gold standard’ for phenotyping.
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The presence of a dicentric chromosome was confirmed by Southern blot analysis of orthogonal field alternating gel electrophoresis gels.
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The diagnosis is confirmed by serum and/or protein immunoelectrophoresis, which reveals an increased amount of immunoglobulin gamma heavy chain not associated with light chain.
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Svedberg's investigations with the ultracentrifuge and Tiselius's electrophoresis studies (see Section 3.10) were instrumental in establishing that protein molecules have a unique size and structure, and this was a prerequisite for Sanger's determination of their amino-acid sequence and the crystallographic work of
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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After centrifugation, the cell pellet was processed for microgel electrophoresis to determine sensitivity to mutagens.
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This is because separation according to the isoelectric point and the molecular weight are carried out through time-consuming gel electrophoresis analysis.
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Serum immunoelectrophoresis did not reveal hyper - proteinemia or paraproteinemia.
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A new type of ablation is based on a technique called electrophoresis, which involves using electrical energy to alter cell membranes and allow chemotherapy drugs to enter more freely.
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For gel blot analysis, 10 g of total RNA was applied to each lane and separated by electrophoresis.
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Fluorophore-assisted carbohydrate electrophoresis (FACE) is a simple and inexpensive method for separating the carbohydrates.
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The results of liver tests were normal, as were those of a chemistry panel and hemoglobin electrophoresis.
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The abnormal variant in both cases bound heparin normally but failed to bind dermatan sulfate as demonstrated by crossed immunoelectrophoresis.
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Dyck and Craig employed capillary electrophoresis as the medium for single-molecule enzyme isolation.
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Electron microscopy, gel electrophoresis, and differential scanning calorimetry show that such fusion involves lipid vesicle formation and dissociation of monomolecular lipid-poor protein.
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The purity of the double-stranded oligonucleotides was confirmed by polyacrylamide gel electrophoresis.
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The immunoglobulin type can be determined by immunoelectrophoresis or immunofixation electrophoresis.
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For gel blot analysis, 2 g of poly + RNA were applied to each lane and separated by electrophoresis.
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Although fat ingestion only slightly affects cholesterol levels, both the triglyceride and lipoprotein electrophoresis results are greatly affected.
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The amplified DNA then undergoes a technique to ascertain the number of repeats at a given loci location, called electrophoresis, which separates the molecules according to length.
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Agarose gel electrophoresis was carried out in sodium barbitone buffer, 2 mM EDTA, pH 8.6, using a 1% agarose gel.
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The latest development is "keiki gels" -- agarose gel electrophoresis using nothing but a drinking straw, a 9V battery and a pair of alligator clips.
Boing Boing
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Appropriate hemoglobinopathy screening should include a complete blood count and hemoglobin electrophoresis.
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The size and purity of the amplified product were checked by agarose gel electrophoresis, and the fragment was purified from the gel by commercially available procedures.
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The use of polyacrylamide gel electrophoresis also can be used to determine the isoelectric charge of proteins (pI).
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Electrophoresis is a technique that separates macromolecules according to their net electrical charge and shape.
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is titled" Selective isolation of live/dead cells using contactless dielectrophoresis
THE MEDICAL NEWS
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Among these methods were ultracentrifugation, X-ray crystallography, electron microscopy, electrophoresis, macromolecular sequencing, and radioactive tracing.
Gene
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The purified protein migrated with an apparent molecular weight of 42 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis.
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Method Apoptosis was assessed by DNA gel electrophoresis, oil - immersion microscopy.
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Proteins were separated by sodium dodecyl sulphate/polyacrylamide gel electrophoresis as described previously.
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Three microliters of the reaction mixture was loaded onto 6.5% polyacrylamide denaturing gels without formamide, and the PCR products were separated by electrophoresis at 1600 V for 4 to 7 hours.
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DEP traps can use either negative dielectrophoresis pushing cells away from the electrodes or positive dielectrophoresis pulling cells toward the electrodes.
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Fluorophore-assisted carbohydrate electrophoresis (FACE) is a simple and inexpensive method for separating the saccharide.
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Samples were digested with the appropriate restriction endonuclease and were size fractionated by electrophoresis through 0.9% agarose gels.
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In the final step, the investigator cuts plugs to fit the wells of an agarose electrophoresis gel.
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Gel electrophoresis, including isoelectric focusing
Laboratory Rotation
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The intactness of RNA was monitored by agarose gel electrophoresis.
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Each step in the curing procedures was monitored by electrophoresis of plasmid isolations.
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Crowdsourced science: HOWTO do agarose gel electrophoresis using nothing but a drinking straw, a 9V battery and a pair of alligator clips
Boing Boing
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Recently, researchers have shown that Dielectrophoresis (DEP) is an efficient technique to manipulate carbon nanotubes.
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Its purity and integrity was assessed using agarose gel electrophoresis.
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Three microliters of the reaction mixture was loaded onto 6.5% polyacrylamide denaturing gels without formamide, and the PCR products were separated by electrophoresis at 1600 V for 4 to 7 hours.
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The paper accepted by the publication "Lab on a Chip" is titled "Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP).
Innovations-report
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All oligos were obtained from Sigma Genosys (Woodlands, TX) and further purified using polyacrylamide gel electrophoresis.
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Charged colloidal particles will migrate in an applied electric field, a process called electrophoresis.
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Electrophoresis equipment and centrifuges halt, causing protein and nucleic-acid bands to diffuse.
The Scientist
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Haemoglobin electrophoresis showed sickle cell trait.
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After electrophoresis, the agarose gels were stained with ethidium bromide and visualized by ultraviolet light.
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In fact domestic dogs and wolves are (based on allozyme electrophoresis) further apart genetically than are leopards and jaguars, and (based on DNA hybridization) further apart than some bear species (Wayne et al. 1991).
Controversial origins of the domestic dog
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P. aeruginosa isolated from the sputum differ phenotypically from the initial intermittent strains even though they produce similar pulse field gel electrophoresis patterns and therefore are considered isogenic
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However, the lessons learned and engineering that went into developing robust and reliable microsystems at SNL was instrumental in motivating his team to come up with a viable solution - called contactless dielectrophoresis (cDEP).
THE MEDICAL NEWS
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Orsborn KI, Shubitz LF, Peng T, Kellner EM, Orbach MJ, et al. (2006) Protein expression profiling of Coccidioides posadasii by two-dimensional differential in-gel electrophoresis and evaluation of a newly recognized peroxisomal matrix protein as a recombinant vaccine candidate.
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Protease samples were submitted to electrophoresis in polyacrylamide gels under denaturing conditions, according to Laemmli.
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The large and small are measured by electrophoresis whereas VLDL is measured by ultracentrifugation.
Carbohydrates or weight loss or both? | The Blog of Michael R. Eades, M.D.
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His paper in the Journal of Molecular Biology describing how he made it is probably the most cited paper in biology, after one by a man called Laemmli, who discovered SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis).
June 2007
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The observation of flow to the tip during both half cycles is clear indication of an additional flow, which is believed to be due to dielectrophoresis.
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Electronic paper (e-paper) utilizes a process called electrophoresis where colored particles in a clear suspension carrying a surface charge are controlled by administering an electric field perpendicular to the surface, forcing them to gather at the top of a pixel and so creating darkened areas.
Gizmag Emerging Technology Magazine
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Results were assayed on a 6% polyacrylamide gel stained with ethidium bromide after electrophoresis.
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Svedberg's student, Arne Tiselius, studied the migration of protein molecules in an electric field, and with this method, named electrophoresis, he demonstrated the complex nature of blood proteins.
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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P. cruentum constructed was characterized to contain lower level of PE content in light of the complete deficiency of γ subunits, the B-PE-associated chromophoric linker polypeptides, revealed by LiDS-PAGE (lithium dodecyl sulfate polyacrylamide gel electrophoresis) experiment
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A universal sulfotransferase assay method based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is described.
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A simultaneous determination of uric acid, creatinine and pseudouridine in human urine by high performance capillary zone electrophoresis(HPCZE)is described.
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Fleischer used gel electrophoresis to discriminate yolk allozymes sampled from eggs laid by several female cowbirds.
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The triplex and biplex PCRs were combined before electrophoresis and loaded onto a single gel lane to form a pentaplex system.
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The mobility decrements may be thought of as shape factors characterizing the apparent curvature of a target fragment, as determined by gel electrophoresis.
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March 10, 2006, 2: 16 am free credit check says: free credit check electrophorus: underloaded, electrophoresis alternated yellower
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Diagnostic and prognostic significance of Pseudomonas aeruginosa precipitins determined by means of crossed immunoelectrophoresis.
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The purity of enzyme preparations was analysed by gel electrophoresis and spectral analysis.
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Specifically, Davalos helped engineer microsystems for the detection of water-borne pathogens using a technique called dielectrophoresis (DEP) in the early part of this decade.
Innovations-report
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The first was protein analysis using sodium dodecyl sulphate polyacrylamide gel electrophoresis.
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The mechanism of the difference in the interreaction of HCPCF and HSPCF and the corresponding complexes has been interpreted by analysing phenomena concerning electrophoresis and phase separation.
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First, after electrophoresis of the proteins through a polyacrylamide gel, they are transferred by blotting to a porous membrane sheet.
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Technicians add 26 short pieces of DNA, called primers: sequences of the chemicals C, A, T and G that link to the beginning and end of each of the 13 sites. 4 Replication: When a primer attaches to the beginning of one of the 13 sites, it acts like the "start" button on a photocopying machine, turning on cellular machinery that makes 1 million copies or more of each site. 5 Identification: Copies of the 13 sites each about 100 to 600 chemical letters long, are separated by size through gel electrophoresis.
Dusting For Dna
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A computer program for calculating migration time and distance of components in arbitrary multistep gradient field capillary gel electrophoresis is compiled on the basis of the result.
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The PCR products were analyzed by electrophoresis on agarose gels containing ethidium bromide and were photographed under ultraviolet transillumination.
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We demonstrate that electrodeless dielectrophoresis can be used for concentration and patterning of both single-strand and double-strand DNA.
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As it can be seen on the electrophoresis gel, the frank scission is highly selective.
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During conventional electrophoresis, molecules larger than 50 kb have essentially the same motility and remain unseparated.
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Cell morphology and DNA gel electrophoresis were used to observe the apoptosis.
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The first was protein analysis using sodium dodecyl sulphate polyacrylamide gel electrophoresis.
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Key to this analysis are the electronics, firstly the dielectrophoresis which forces the cells together so doctors can observe interactions between them.
PhysOrg.com - latest science and technology news stories
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This revised and expanded edition has two new chapters on capillary electrophoresis and supercritical fluid chromatography.
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They separated the DNA out of the blood and analyzed it with conformational gel electrophoresis.
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Methods By using an uncoloured affinity-electrophoresis combined with monoclonal double antibody sandwich enzyme immunoassay.
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Under the leadership of Tiselius this institute has contributed to the development and improvement of a number of useful methods in biochemistry, such as electrophoresis, chromatography, phase partition, gel filtration, etc.
Arne Tiselius - Biography
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We assign this trapping to strong dielectrophoresis due to the high electric field and electric field gradient in the pipette tip.
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The pattern of serum protein electrophoresis results depends on the fractions of two major types of protein: albumin and globulins.
