How To Use Cdna In A Sentence
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Several deletion mutants were prepared by exploiting singular restriction sites in the cDNA to enable progressive sequencing.
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We then conducted competitive hybridization experiments of cDNAs between a head portion and the other body portion of planarians in order to screen genes specifically expressed in a planarian brain.
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The complete stoned cDNA was obtained by ligation of fragments from three different cDNA clones.
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We observed an accumulation of cDNA loci near the centromere for seven chromosomes.
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At that time, however, it was commonly believed that expressing the cDNA in living organisms would not produce fluorescent GFP, because the formation of its chromophore requires the reactions of condensation and dehydrogenation that are not expected to occur spontaneously.
Osamu Shimomura - Autobiography
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Multiple cDNA clones were identified by screening the U251 library with a bacterial lysate absorbed polyclonal antiserum against human tenascin.
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This strategy is based on the attachment of some cytidine nucleotides to nascent cDNA first strands by reverse transcriptase.
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Liao YF, Kudo S, Eto S, Mattei MG, et al. (1995) Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme.
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This cDNA fragment was used to screen several murine cDNA libraries.
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The GAACGACTCTACAATGTTGTtGTAGAtACAGAAGTTACTG GTAAAAAG oligonucleotide was used to mutagenize full-length SMC2 cDNA (a gift from K. Yokomori), which was subsequently inserted into NheI and XhoI sites of pcDNA3. 1, to produce the pcDNA3. 1/SMC2-2 complementation plasmid, resistant to RNAi with RNA duplex #2, due to silent mismatch mutation at the siRNA-corresponding site.
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The cDNA-amplified fragment length polymorphism approach was used to identify genes expressed differentially during early and late flower bud development in tea plant(Camellia sinensis).
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The 3' untranslated trailer sequence is 937 nucleotides long and no poly A tail is present indicating internal priming during cDNA synthesis.
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Positive phage clones were converted to plasmid vectors using the material provided in the cDNA synthesis kit.
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Over 20 oviparous specifically expressed genes were identified through screening the subtracted cDNA library enriched between oviparous and ovoviviparous
Journal of Biological Chemistry current issue
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Partial cDNA clones are shown by dotted lines at the 5' end; translation termination codons are indicated by an asterisk.
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This mutation does not prevent transcription of the gene since we were able to obtain full-length mutant cDNA.
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A cDNA library of mRNA isolated from asparagus spears 24 h post-harvest was screened for peroxidases using homologous and heterologous probes.
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The fragments were cloned and DNA sequencing demonstrated that they actually corresponded to cDNA with proper intron splicing.
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A cDNA library could contain all cDNAs from a single tissue, for example, and could be screened for the ability to hybridize to a fragment of known DNA.
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Several deletion mutants were prepared by exploiting singular restriction sites in the cDNA to enable progressive sequencing.
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Only full-length genomic and cDNA clones, including genes with in-frame stop codons, were analyzed.
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Partial cDNA clones are shown by dotted lines at the 5' end; translation termination codons are indicated by an asterisk.
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A cDNA fragment was isolated and cloned from the total RNA of calli of Onobrychis tanaitica containing condensed tannin using RT-PCR technique.
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At that time, however, it was commonly believed that expressing the cDNA in living organisms would not produce fluorescent GFP, because the formation of its chromophore requires the reactions of condensation and dehydrogenation that are not expected to occur spontaneously.
Osamu Shimomura - Autobiography
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We will also pursue further studies delineating immediate early gene/cytokine/chemokine expression in human partial liver grafts utilizing cDNA microarrays on liver biopsies obtained after reperfusion, in both cadaveric and living donor partial grafts.
Basic Biliary Atresia Research
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Amplified fragments were cloned, sequenced and confirmed as being derived from the relevant cDNA.
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The cDNA clones were sequenced from both ends using standard forward and reverse primers.
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We also found 12 unique toothfish γ crystallin cDNAs (twice that found in mammals), some of which are homologues of the γ crystallins from warm bodied mammals.
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The study of full-length cDNA remains an indispensable approach for determining the structure of genes.
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Therefore, we cloned a fragment of the yolk protein cDNA.
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Molecular markers derived from cDNA and genomic DNA clones were from the tomato high-density linkage map.
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First-strand cDNA was synthesized with an oligo primer from total RNA extracted from scutella of germinating seeds.
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R.M.; Croteau, R.B.: Molecular clon - ing of a cytochrome P450 taxane 10b-hydroxylase cDNA from Taxus and functional expression in yeast.
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On-column DNase I digestion was performed. 100 ng of each RNA sample was retrotranscribed with QuantiTect Whole Transcriptome Kit (Qiagen) and a 50-fold dilution of cDNAs was analyzed by quantitative Real Time PCR (qRT-PCR) using an iCycler-iQ5
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An frt site-flanked selection cassette, with a neomycin resistance gene, the Flpe cDNA cloned under control of the ACE promoter
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Crab A, Meiring M, Pritchard D, Deckmyn H (2001) Identification of a collagen-binding protein from Necator americanus by using a cDNA-expression phage display library.
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Genomic and cDNA CAPS analyses indicated that plants representing multiple populations of this young natural allopolyploid have experienced frequent and preferential elimination of homeologous loci.
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Synthesis of cDNA for the array screening assays was performed on 2 µg of total RNA using a T7-oligo dT12-18 primer (Amersham Pharmacia) and it was purified using phenol: chloroform: isoamyl alcohol and NH
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Walter P, Kumar V, Krust A, Bornert JM, et al. (1986) Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A.
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Renilla and firefly luciferase genes to create pcDNA3. 1-hODC-IRES. pcDNA3. 1-V5-ZNF9 was constructed by insertion of the PCR-generated full-length open reading frame of human ZNF9 into pENTR and subsequent recombination into pcDNA3. 1/nV5-Dest (Invitrogen) by recombinational cloning (Invitrogen).
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The cDNA samples were pipetted into separate tubes and stored at - 80°C.
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Fibroblasts infected with retroviral constructs expressing the indicated cDNA (top) were harvested 9 days postinfection, and whole cell extracts assayed by western blot with the indicated antibodies (left).
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Another group is formed by cDNAs encoding enzymes with putative biosynthetic functions.
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Recombinant cDNA were analyzed by differential screening.
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Filters were probed with 32P-labeled HBVayw genomic DNA to detect HBV sequences and mouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH) cDNA to detect the GAPDH transcript used as an internal control
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The cDNA synthesis was performed at 42° for 30 min using 1 g of total RNA and an oligo primer.
