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How To Use cDNA In A Sentence

  • Several deletion mutants were prepared by exploiting singular restriction sites in the cDNA to enable progressive sequencing.
  • We then conducted competitive hybridization experiments of cDNAs between a head portion and the other body portion of planarians in order to screen genes specifically expressed in a planarian brain.
  • The complete stoned cDNA was obtained by ligation of fragments from three different cDNA clones.
  • We observed an accumulation of cDNA loci near the centromere for seven chromosomes.
  • At that time, however, it was commonly believed that expressing the cDNA in living organisms would not produce fluorescent GFP, because the formation of its chromophore requires the reactions of condensation and dehydrogenation that are not expected to occur spontaneously. Osamu Shimomura - Autobiography
  • Multiple cDNA clones were identified by screening the U251 library with a bacterial lysate absorbed polyclonal antiserum against human tenascin.
  • This strategy is based on the attachment of some cytidine nucleotides to nascent cDNA first strands by reverse transcriptase.
  • Liao YF, Kudo S, Eto S, Mattei MG, et al. (1995) Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme. PLoS ONE Alerts: New Articles
  • This cDNA fragment was used to screen several murine cDNA libraries.
  • The GAACGACTCTACAATGTTGTtGTAGAtACAGAAGTTACTG ​ GTAAAAAG oligonucleotide was used to mutagenize full-length SMC2 cDNA (a gift from K. Yokomori), which was subsequently inserted into NheI and XhoI sites of pcDNA3. 1, to produce the pcDNA3. 1/SMC2-2 complementation plasmid, resistant to RNAi with RNA duplex #2, due to silent mismatch mutation at the siRNA-corresponding site. PLoS ONE Alerts: New Articles
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