How To Use Ultracentrifuge In A Sentence
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Svedberg's investigations with the ultracentrifuge and Tiselius's electrophoresis studies (see Section 3.10) were instrumental in establishing that protein molecules have a unique size and structure, and this was a prerequisite for Sanger's determination of their amino-acid sequence and the crystallographic work of
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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The homogenate, obtained after sonication, was ultracentrifuged at 60 000 g for 30 min.
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The sample was then ultracentrifuged for 1 hour at 141,000 × g to remove membranes.
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An additional 34 ml buffer solution was added and the sample was then ultracentrifuged at 100,000 × # for 4 h.
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Svedberg's investigations with the ultracentrifuge and Tiselius's electrophoresis studies (see Section 3.10) were instrumental in establishing that protein molecules have a unique size and structure, and this was a prerequisite for Sanger's determination of their amino-acid sequence and the crystallographic work of
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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Boundary sedimentation and sedimentation equilibrium experiments were performed using an analytical ultracentrifuge Optima XL-I from Beckman Instruments (Palo Alto, CA) equipped with absorbance and interference optics.
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Perrin of Sorbonne for developing equilibrium sedimentation in colloidal solutions, a method which Svedberg later perfected in his ultracentrifuge.
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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To concentrate the solution, the collagen was ultracentrifuged at 10 deg C for 26-48 It for preparation of 10-45 mg/ml gels.
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The remaining sample was ultracentrifuged to obtain the sputum sol phase.
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The supernatant was recentrifuged at 109,000 g on a tabletop ultracentrifuge (Rotor TLA 45 of Beckman centrifuges, Beckman Coulter, Fullerton, CA) for 1 h.
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After removing the cell debris by centrifugation, supernatant was layered on a 5.7 M CsCl / 0.01 M EDTA solution in an ultracentrifuge tube and centrifuged in a swinging bucket rotor at 40,000 rpm for 16 hr.
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The sarcosine insoluble fractions were removed by centrifugation at 100,000 g for 1 h in an ultracentrifuge, washed once with PBS, pH 7.4 and used as outer membrane protein fractions.
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PLY solution species from monomer via multimeric intermediates to ring-shaped oligomers were studied with time-dependent sedimentation velocity in the analytical ultracentrifuge.
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The supernatant was ultracentrifuged at 105,000 × g, 4°C for 1.5 h.