How To Use Trypsin In A Sentence
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The results showed that the matrix can adsorb L-asparaginase and recover high enzymic activity, The immobilize enzyme was more stable against trypsin than native enzyme.
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After 8 days, nonadherent cells were removed and adherent cells were detached with 0. 05% trypsin/0.53 mM EDTA and replated.
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Examples of important proenzymes include pepsinogen, trypsinogen and chymotrypsinogen, which give rise to the proteolytic digestive enzymes.
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Many people take extra doses of naturally produced enzymes, such as lactase, pepsin and trypsin, in supplement form.
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These outer hair cells are trypsin treated from inside and are made spherical by inflation.
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The active site of trypsin involves a catalytic triad consisting of the amino acids serine, histidine and aspartic acid.
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Nevertheless, it is plausible that the complex kinetic profile of tryptic digestions of G-actin results from a combination of factors, including trypsin inhibition and the presence of several subpopulations of actin states.
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The proteins are chopped into smaller fragments using an enzyme called trypsin; these fragments are then vaporized, ionized, and shot through an electric field.
Ars Technica
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The digestive juices [Footnote 47: The pepsin and hydrochloric acid of the stomach, the trypsin of the pancreatic juice, and the erepsin of the intestinal juice digest proteins.] of these organs change protein into soluble forms.
School and Home Cooking
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The completion of the sequence depended on the use of specific proteases - trypsin, chymotrypsin, and pepsin - to produce larger fragments.
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Many people take extra doses of naturally produced enzymes, such as lactase, pepsin and trypsin, in supplement form.
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Steigemann (also one of Huber's Ph.D. students at that time) on the crystallographic refinement of the structure of bovine pancreatic trypsin inhibitor was a success, and our 1975 paper in
Johann Deisenhofer - Autobiography
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To summarize, metaphase spreads were made from a 24-hour, unstimulated cell culture and were G-banded by the standard trypsin-Giemsa banding method.
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Trypsin also activates trypsinogen as well as all the other proenzymes in the pancreatic secretion, producing the active proteases and peptidases that hydrolyze dietary polypeptides.
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Princeton began to work on the purification of the protein-splitting enzymes met with in the digestive apparatus and gradually succeeded in obtaining a number of them in crystallized form, e.g. the pepsin met with in the gastric juice and the trypsin and chymotrypsin in the pancreas.
Nobel Prize in Chemistry 1946 - Presentation Speech
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Next, the smaller proteins move into the small intestine, where pancreatic enzymes, trypsin and chymotrypsin, continue the breakdown of proteins into amino acids.
Carrie Wiatt, M.S.: Fruit: Fads, Digestion and Cleanses
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This is because the pancreatic ferment (trypsin) has digested the casein into "peptone," which does not curdle.
A Practical Physiology
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These substances may be natural constituents of the seeds, such as gossypol and cylopropenoid fatty acids in cottonseed, cyanogenetic glycoside in linseed, ricin in castor beans, sinigrin or sinalbin in mustard seed, saponin in shea nuts, the trypsin inhibitor in soyabeans, or toxic mould metabolites, such as aflatoxin, which may form if the seeds are allowed to spoil by moulds.
Chapter 7
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Montgomery etal isolated cells from 18 day fetal rat intestine by trypsin dissociation.
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Most notably, there are the proteolytic enzymes trypsin and chymotrypsin, which our bodies make.
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Take the alpha one antitrypsin enzyme deficiency in the lung.
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All the three spice mixes when consumed through the diet favorably enhanced the pancreatic lipase, chymotrypsin and amylase activities.
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The most common allergen in soy is called trypsin inhibitor.
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You also have to eat alot of foods like sweet potatoes, which have a chemical called trypsin inhibitor in them.
Archive 2007-03-01
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The chymotrypsin crystals were twinned and therefore difficult to work with, and so Perutz continued with haemoglobin.
Max F. Perutz - Biography
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The solids include mineral salts (the chief of which is sodium carbonate) and four different chemical agents, or enzymes, — trypsin, amylopsin, steapsin, and a milk-curding enzyme.
Physiology and Hygiene for Secondary Schools
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Tomita uses a digestive enzyme called trypsin, along with a host of other chemicals, to break down the proteins and muscles, halting the process just at the moment they become transparent but before they lose their form.
Wired Top Stories
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Fifty-micrometer sections of paraffin-embedded, 10% neutral buffered formalin fixed tissues were dewaxed and rehydrated, and a whole nuclei prep was made by digesting with trypsin.
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The fibrinolytic system involves a series of enzymatic reactions that results in the conversion of the proenzyme, plasminogen, into the trypsin-like lyric enzyme, plasmin.
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Benzamidine bound to trypsin has a buried positively charged amidinium group and a more solvent-accessible nonpolar phenyl ring.
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The proteases are secreted in forms of zymogen (ie, inactive precursor to enzyme), such as trypsinogen, chymotrypsinogen, and procarboxypeptidase.
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They then added the enzyme trypsin to carve up the remaining proteins into small, but still recognizable fragments, or peptides.
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A recent study published in Clinical Rheumatology showed that a combination of the amino acids trypsin and bromelain combined with rutin relieved pain just as well as many drugs.
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Mesotrypsinogen comprises a minor portion of the total trypsinogen protein in the pancreas.
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The cells were passaged by trypsinization with 0.05% trypsin-EDTA and used for experiments at passages 3-6.
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Selected cases were also examined with immunohistochemical stains for lipase, trypsin, and amylase.
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These substances may be natural constituents of the seeds, such as gossypol and cylopropenoid fatty acids in cottonseed, cyanogenetic glycoside in linseed, ricin in castor beans, sinigrin or sinalbin in mustard seed, saponin in shea nuts, the trypsin inhibitor in soyabeans, or toxic mould metabolites, such as aflatoxin, which may form if the seeds are allowed to spoil by moulds.
Chapter 7
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The Manitoba-based researchers prepared different protein hydrolysates using different enzymes, including pepsin, ficin, trypsin, papain, thermolysin, pancreatin and Alcalase.
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Testes from 18-day-old embryonic chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion with collagenase and trypsin.
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Many people take extra doses of naturally produced enzymes, such as lactase, pepsin and trypsin, in supplement form.
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Trypsin favors basic residues like lysine and arginine; chymotrypsin favors aromatic residues like phenylalanine, tyrosine, and tryptophan.
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This site is well characterized in trypsin and other serine proteinases.
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The five diseases studied by the CLiC team are: Alagille Syndrome (AGS), Alpha-1-antitrypsin deficiency (a-1AT), Bile acid synthesis and metabolism defects, Mitochondrial hepatopathies, and Progressive familial intrahepatic cholestasis (PFIC).
Links and Resources for Alagille Syndrome
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In 1929 he isolated pepsin in pure crystalline form by techniques which were later used by himself and other workers to crystallize trypsin, chymotrypsin, carboxypeptidase, and pepsinogen.
John H. Northrop - Biography
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Binding affinities of a series of benzamide inhibitors for trypsin were evaluated by molecular dynamics (MD) simulations using a linear response approach.
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Raw soybeans, however, contain a substance called a trypsin inhibitor.
9: Domestic animals
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Furthermore, a known soy allergen called trypsin inhibitor was as much as 7 times higher in the toasted GM soy, compared to non-GMO soy!
Jeffrey Smith: Biotech Propaganda Cooks Dangers Out of GM Potatoes
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Proteinase inhibitors, affecting trypsin and chymotrypsin, have been found in seeds of A. cowleana and other species. but only at levels similar to those found in peas or beans-much lower than levels in soybeans or winged beans (Kortt, 1984).
Chapter 48
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Northrop, however, managed to crystalize three digestive enzymes, pepsin, trypsin and chymotrypsin, and by painstaking experiments shown them to be pure proteins.
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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As trypsinogen is secreted into the duodenum, it is changed into trypsin by enterokinase.
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Oilseeds often contain toxic or undesirable factors such as gossypol in cottonseed, trypsin inhibitor in soya beans and cyanogenetic glucosides in linseed, whereas groundnuts have been noted as being particularly vulnerable to mycotoxin formation.
Chapter 6
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Some manufacturers are adding proteolytic enzymes (enzymes that break down proteins), such as bromelain, papain, pancreatin and trypsin, to their protein powders.
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Examples of important proenzymes include pepsinogen, trypsinogen and chymotrypsinogen, which give rise to the proteolytic digestive enzymes.
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Get a product that contains pancreatin, trypsin, papain or bromelain, and follow label for exact dosage.
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The most likely and simplest explanation is that when cooked meat is ingested, all the proteins have become non-soluble and can not be made soluble except by the action of digestive juices: pepsin, trypsin and erepsin.
Charles Richet - Nobel Lecture
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At least two small clinical trials, both fundedby the manufacturer, have tested a supplement containing bromelain plus two other substances that theoretically might ease arthritis — the enzyme trypsin and the antioxidant rutin.
Q&A: Pineapple pills for arthritis pain?
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You can read an abstract of the study, Pancreatitis-associated chymotrypsinogen C (CTRC) mutant elicits endoplasmic reticulum stress in pancreatic acinar cells, online in the November
PhysOrg.com - latest science and technology news stories
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a product made from a transgenic plant-a protein called trypsin produced in corn kernels and sold to the pharmaceutical industry for mammalian cell culturing.
News from The Scientist
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Approximately 300 dechorionated embryos were collected in tubes containing 500 µl of a 1× Trypsin / EDTA solution, and were digested by pipetting for 5 to 10 min.
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(ProXCISION; PerkinElmer), reduced, alkylated, and digested overnight with bovine trypsin
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Alpha-1 antitrypsin deficiency and Wilson disease were excluded by determining serum concentrations of alpha-1 antitrypsin and ceruloplasmin, respectively.
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The chymotryptic digestion of the trypsin-resistant core of haemoglobins A and S.
The Edge of Evolution
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The enzymatic dissociation was completed in a trypsin solution, after which individual cells were isolated from the digested tissue by trituration.
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The material may have come from a pig-derived enzyme called trypsin used early in development,
Arab Times Kuwait English Daily
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Oilseeds often contain toxic or undesirable factors such as gossypol in cottonseed, trypsin inhibitor in soya beans and cyanogenetic glucosides in linseed, whereas groundnuts have been noted as being particularly vulnerable to mycotoxin formation.
Chapter 6
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Ibrahim had shown that trypsin is not present till seven or eight months of intrauterine development.
Frederick G. Banting - Nobel Lecture
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Hypercalcemia increases calcium, which is believed to activate trypsinogen.
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Trypsin is non-active when it has not been in contact with enterokinase.
Charles Richet - Nobel Lecture
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A report on the effect of spices on digestive enzymes mentioned that freshly prepared 1 per cent emulsion of asafoetida in water affected the activity of pepsin, trypsin and rennin in saliva to a small extent 41.
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Examples are the digestive enzymes trypsin and chymotrypsin, which only have enzymatic activity after the protein chain is cut and fragments of the protein removed.
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This group, including trypsin and chymotrypsin, evolved from a common ancestral protease, and all use the same mechanism of action at their active site.
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Action organic lipid solvents, saponin trypsin virus ecthyma contagiosum
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The alternative possibility, that the kinetic complexity of Mg-G-actin digestions arises from trypsin inhibition in these reactions, has been tested in two ways.
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The solids include mineral salts (the chief of which is sodium carbonate) and four different chemical agents, or enzymes, — trypsin, amylopsin, steapsin, and a milk-curding enzyme.
Physiology and Hygiene for Secondary Schools
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Trypsin, the hydrdytic enzyme of insects, was crucial for digestion and toxicity of Cry protein.
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We obtained chymotrypsin from Sigma Chemical, where it is purified from bovine pancreas.
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When the two loops in trypsin were changed into chymotrypsin loops, the hybrid protein shows chymotrypsin-like cooperatvity.
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Single stomach smooth muscle cells were isolated by enzymatic dissociation with trypsin and collagenase as described previously.
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Immunohistochemistry showed that the tumor cells were diffusely reactive to alphafetoprotein, alpha 1, antitrypsin, and cytokeratin.
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Biochemical pharmaceutical preparations workshop main products are trypsin, stomach enzymes and pharmaceutical intermediates, and so on.
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We found that both trypsin and elastase digestion eliminated counterbend formation.
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Alpha-1-antitrypsin deficiency: a hereditary disease that can cause hepatitis and liver failure.
Who needs a liver transplant?
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Using techniques including atomic force microscopy and mass spectrometry, the team found that very similar enzymes, known as trypsin-like serine proteases, are at work in barnacle glue.
Signs of the Times
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Bernal, Fankuchen, and Perutz [Nature, 141 (1938) 523] published a joint paper on X-ray diffraction from crystals of haemoglobin and chymotrypsin.
Max F. Perutz - Biography
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Trypsin was covalently linked with chemical modified granule chitosan and was used to isolate and purify aprotinin from the extract of cattle lungs by affinity chromatography.
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In 1970, I had begun work on the basic pancreatic trypsin inhibitor which has later become the model compound for the development of protein NMR, molecular dynamics, and experimental folding studies in other laboratories.
Robert Huber - Autobiography
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Pancreatic trypsin is a member of the large and diverse serine peptidase family.
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Montgomery etal isolated cells from 18 day fetal rat intestine by trypsin dissociation.
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The most frequently used biological molecules included in the digestion models were digestive enzymes (pancreatin, pepsin, trypsin, chymotrypsin, peptidase, α-amylase, and lipase), bile salts, and mucin.
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In this study, we have used the Gaussian network model and a clustering method to analyze the dynamic properties of trypsin and chymotrypsin.
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Cells were passaged twice weekly using a standard trypsinization / EDTA protocol.
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In 1929 he isolated pepsin in pure crystalline form by techniques which were later used by himself and other workers to crystallize trypsin, chymotrypsin, carboxypeptidase, and pepsinogen.
John H. Northrop - Biography
