How To Use Resuspend In A Sentence
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Briefly, single yeast colonies harboring the TNR sequences were resuspended in water and appropriate dilutions were plated onto nonselective media.
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Platelets were resuspended in washing buffer at appropriate concentrations for platelet aggregation measurement.
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In the experiment, FAC was removed by washing the co-stimulated tetrahymena and resuspending them in fresh media.
2010 April - Telic Thoughts
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The solvent was removed by evaporation with a N 2 stream and the dried lipid film was resuspended in decane to form a final total lipid concentration of 25 mg/ml.
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resuspend particles
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There is nothing to slow the wind-caused currents or keep the sediments from resuspending.
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For flowcytometric analysis, CD3+ were kept on ice and washed extensively with citrated PBS containing 1% FCS. 10 µl of FITC, APC or PE - conjugated antibody was added and incubated on ice for 20 min. 300 µl FACS buffer was added and T cells were pelleted, resuspended in 200 µl buffer.
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Surgeons have advanced in technique to shape and contour the deeper facial tissues and resuspend them.
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Such nuclear suspension was sedimented by gentle centrifugation, the enzyme solution was decanted, and the nuclear pellet was resuspended in 1 x PBS buffer.
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This residual volume was vortexed vigorously for 5 seconds at medium speed to resuspend the cells and cellular debris.
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However, the sediment is not usually resuspended when a ship discharges its ballast.
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It was hypothesized that the water used to resuspend the nucleic acid was not at the proper pH.
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Tidal processes constantly winnow the substrate surface in the submerged mouthbar front, and resuspend mud and silt into the water.
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We evaporated the aqueous buffer from the microspheres and resuspended the microspheres inside silicone oil.
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Cells from new zygotic colonies were resuspended in 1 ml water and appropriate dilutions were made.
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Finely divided pulp debris and dead yeast cells which are very small settle more slowly and are easily resuspended by currents within the settling vessel.
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The flocs dispersed immediately when cells were resuspended in a glucose-citrate buffer.
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Purified effector cells were washed twice with Hank's balanced salt solution (HBSS), enumerated using trypan blue, and resuspended in PBS at 1-5. 10 7 cells mL − 1, depending on the quantity of PMN collected on a given day and the number of wells tested.
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The resuspended pellets were placed into 35 mm petri-dishes and then sealed in a desiccator over silica-gel at 30°C for 58 days.
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Supernatant fractions were removed and TCA precipitated as described above, and pellet fractions were resuspended in solubilization buffer.
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This fixation step was repeated twice and the resulting cells were resuspended in a small volume of fixative solution and carefully placed onto clean slides.
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The fixative solution was partially but not completely removed, the pellet was resuspended, dropped onto pre-cleaned microscope slides and dried for 24 h at room temperature.
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The nitrated peptides were eluted with 50% acetonitrile/0. 2% trifluoroacetic acid, dried, resuspended in 1.5 µl of matrix suspension (4 mg α-cyano-4-hydroxycinnamic acid, 0.8 mg 3,5-dihydroxybenzoic acid in 50% acetonitrile/0. 1% trifluoroacetic acid, 0.1 mM fucose), and deposited on a stainless steel target.
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Yeast cultures were pelleted, deflocculated twice by washing with buffer and resuspended in the same buffer at a density of 10 cells/ml.
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Cells were resuspended in an equal volume of polysome lysis buffer
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The pellets were resuspended and two aliquots withdrawn for analysis.
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Cells were released from the arrest by filtration and resuspended in fresh media with raffinose and galactose.
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The IP pellet was resuspended in final wash buffer and aliquoted for subsequent biochemical analyses.
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All extracts were centrifuged at 10000 rpm for 5 min, and the supernatants were then evaporated under vacuum to dryness and resuspended in appropriate solvents to a final concentration of 1 mg/ml.
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Cells were resuspended in polysome buffer (120 mM KCl, 2 mM MgCl
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The carbodiimide was washed with a phosphate buffer and the beads were resuspended in a borate buffer.
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The following day, cells were harvested by centrifugation, resuspended in buffer (20 mM Tris-HCl, pH 8.0; 100 mM NaCl), and disrupted by sonication.
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Tidal processes constantly winnow the substrate surface in the submerged mouthbar front, and resuspend mud and silt into the water.
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Supernatant fractions were precipitated with trichloroacetic acid, washed with acetone, and resuspended in ribosome solubilization buffer.
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The DNA pellet was resuspended in piperidine and maintained at 95 deg C for 20 min.
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Briefly, single yeast colonies harboring the TNR sequences were resuspended in water and appropriate dilutions were plated onto nonselective media.
