How To Use Plasmid dna In A Sentence
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Plasmid DNA was prepared using the Qiagen plasmid purification system.
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The predicted 5' and 3' boundaries of the expression regions were confirmed by direct plasmid DNA sequencing.
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The lactic acid bacteria naturally contain from one to more than ten distinct plasmids, and metabolically important traits, including lactose-fermenting ability, bacteriophage resistance, and bacteriocin production, have been linked to plasmid DNA.
1 Upgrading Traditional Biotechnological Processes
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Liposomal formulations of genetic drugs such as antisense oligonucleotides and plasmid DNA are difficult to achieve.
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Unwanted materials, including RNA, DNA, and proteins, are thoroughly washed from the column before the plasmid DNA is eluted using a high salt buffer.
Archive 2005-10-01
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Twenty-four hours after transfection, the cells were harvested and the plasmid DNA recovered.
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Alcohol (isopropanol) precipitation concentrates the eluted plasmid DNA, which is collected by centrifugation, washed, and redissolved in buffer.
Archive 2005-10-01
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Alcohol (isopropanol) precipitation concentrates the eluted plasmid DNA, which is collected by centrifugation, washed, and redissolved in buffer.
Archive 2005-10-01
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Recently, simplified ion-exchange column chromatography methods (commonly termed a mini-prep) have largely supplanted the cumbersome and time-consuming centrifugation protocol to yield large quantities of endotoxin-free plasmid DNA in a relatively short period of time.
Archive 2005-10-01
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Asuragen is the preeminent partner for contract development and GMP manufacturing of RNA - and DNA-based standards and controls including plasmid DNA, Armored RNA®, and capped and uncapped IVT RNA.
The Earth Times Online Newspaper
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These techniques range from simple calcium phosphate precipitation to sequestering the plasmid DNA in lipid vesicles that fuse to the cell membrane and deliver the contents to the cytoplasm (as illustrated in Figure 8).
Archive 2005-10-01
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Alcohol (isopropanol) precipitation concentrates the eluted plasmid DNA, which is collected by centrifugation, washed, and redissolved in buffer.
Archive 2005-10-01
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The plasmid DNA was then circularized with ligase and used to transform E. coli.