How To Use Pipet In A Sentence
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[12] The spermatozooids of certain plants can be strongly attracted towards a pipette which is filled with malic acid -- crowding around and into it with avidity.
Darwin, and After Darwin (Vol. 1 and 3, of 3) An Exposition of the Darwinian Theory and a Discussion of Post-Darwinian Questions
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The micropipette was pulled manually to a gradual taper to ease insertion into the tissue.
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Right now I don't think popular cell probing techniques such as micropipette aspiration, magnetic twisting cytometry and AFM can provide the material constants needed for the commercial package.
IMechanica - Comments
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These are the plants characterized by sympetalous corollas, epipetalous stamens, and having the number of stamens equal or less than the number of corolla lobes.
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Snap off the remainder of the capillary tube from the broad upper portion of the pipette which is now destined to form the covering tube or air chamber, or what we may term the "barrel.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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When the sealed ends are cold and the blood has clotted, place the pipette on the centrifuge, clean end downward; counterpoise and centrifugalise thoroughly.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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Pollen was sedimented by centrifugation at 100 g for 2 min and the excess sucrose drawn off by pipette.
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Wearing blue gloves, he uses a pipettor -- like a giant syringe -- to withdraw a small amount of liquid from a microcentrifuge and inject it into a tiny tray.
Postbulletin.com Local News
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Specially prepared micropipettes are then used to aspirate sperm from these tubules.
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The apertures replace the tip of glass pipettes commonly used for patch clamp recording.
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Stagiaires with small cups of herbs elbow others with pipettes of oil out of the way in order to get to the plate, while still others, momentarily bereft of anything to do, crowd around trying to watch.
The Sorcerer’s Apprentices
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Next, they took an unfertilized egg from a second sheep and removed its DNA, carefully sucking it out with a thin glass tube called a pipette.
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Advance has been along two lines, markedly in relation to insect-pollination, one of which has culminated in the hypogynous epipetalous bicarpellate forms with dorsiventral often large and loosely arranged flowers such as occur in Scrophulariaceae, and the other in the epigynous bicarpellate small-flowered families of which the Compositae represent the most elaborate type.
Encyclopaedia Britannica, 11th Edition, Volume 2, Part 1, Slice 1
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Ten minutes after adding malate, we again pipetted the supernatant from the same dish into other test tubes.
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Here we highlight five of this year's award winners:Katie CleminsonKatie captured the judges' imagination with her dreamy illustrations, which she creates using inks applied with pipettes.
Picturebook perfect
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The pipette was attached to the test tank 2 cm above the water surface, creating a perturbation on the surface when water was dripped into the aquarium.
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The kit contains test tubes, pipettes, funnels and other lab materials necessary for multiple experiments and activities.
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'In a dreary, lonely lab a young female postdoc puts down her pipette to massage her aching latexed hands.
Science
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The elastic moduli K and A of the membrane have been measured by various techniques, among which the micropipette is the most commonly used.
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The company refinement of pipette, the specification is 100 ul - 500 ul , 200 ul - 1000 ul quality assurance, welcome to consult negotiate.
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Single cell preparations were made by vigorously pipetting the cell suspension with a Pasteur pipette and by vortexing.
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Van Steirteghem at the Brussels Free University, involves removing an egg and directly injecting a single sperm into it using microscopic glass needles called pipettes.
Isabel’s Bed
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When is it best to use a graduated cylinder over a buret or pipet?
Yahoo! Answers: Latest Questions
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Using the battery's pipette, you insert your urine and the battery will begin recharging.
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In a third method the interaction between two large vesicles formed at the end of micropipettes and imaged by a light microscope is determined.
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Approximately 300 dechorionated embryos were collected in tubes containing 500 µl of a 1× Trypsin / EDTA solution, and were digested by pipetting for 5 to 10 min.
PLoS ONE Alerts: New Articles
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Automatic dispensing pipettes and micropipettes are also available commercially.
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We then, suspended the embryos in 1 M urea and dissociated them by pipetting.
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The actinomorphic, tubular flowers of both species are pale blue to mauve, with epipetalous anthers.
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Next, each 10 L of ant extract was pipetted onto a filter-paper disk.
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A key feature of this flow field is the kinematic linearity, i.e., doubling the pipette pressure doubles the shear stress on the cell surface.
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By averaging the experimental results over 12 cells, a representative curve relating the membrane extension into the micropipette and the applied suction pressure was generated.
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Cells were both immobilized and controllably deformed by aspiration into a micropipette.
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When aspirated into the micropipette, these GUVs behaved as liquids with no surface shear rigidity.
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Embryonic thymi were digested with collagenase D (1.2 mg/ml, Roche Diagnostics), DNase I (1.5 mg/ml, Roche Diagnostics), and Dispase (1.25 mg/ml, Invitrogen) at 37°C for 15 minutes with occasional gentle agitation with a glass pasture pipette.
PLoS ONE Alerts: New Articles
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Most techniques for monitoring whole-cell membrane capacitance work by applying a voltage stimulus via a patch pipette and measuring the resulting currents.
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Carefully tighten the clamp until it firmly supports the pipette in a vertical position.
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The external water was thoroughly stirred with a pipette for 15 s and the additional fluorescent intensity was measured.
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Use a pipette to make two or three long lines of water down the desk.
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In each case the pipette should be gently inserted and withdrawn from the fluid in order to minimize disturbance.
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This is my composition; the pipette is my clarinet".
Max Delbrück - Nobel Lecture
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Small holes were made in the film for the microscope objective lens and the micropipette.
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In contrast to the model of a rigid microvillus, recent micropipette measurements indicate that neutrophil microvilli might be elastic enough to extend in response to forces that neutrophils might encounter in vivo.
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Relaxed single smooth muscle cells were obtained by gentle trituration of the digested tissue with a plastic pipette.
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By means of a pipette graduated to 0.01 c.c. it is possible to deliver very small quantities; but if the calculated amount runs into thousandth parts of a cubic centimetre, these are replaced by corresponding quantities of normal or even decinormal soda.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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I showed the candidates how to use a pipette - a hand-held tool for manipulating small volumes of liquid - and invited them to have a go.
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The interior of the pipette is connected to an electronic amplifier.
Physiology or Medicine 1991 - Press Release
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I particularly enjoyed the lyrics concerning DNA and the use of a pipet as a prop on the beach.
Archive 2008-07-01
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As if that's not bad enough, my pipettes were pilfered by the student/postdocs and are now broken, overwound and/or covered in a sludge that may or may not be biological waste.
Messy labs - Part 2
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DNA was added by touching a sterile pipette tip to one of the bacterial streaks and twirling it briefly in the reaction mix.
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Once when he tried to suck up liquid in a pipette while testing metals, he accidentally sucked some of the dangerous liquid into his mouth.
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H kingston 1 gb memory revenuer calibrated to lusciousness pipette, hereupon simultaneity, enforceable faeces, rtm counterdemonstrator, disgraceful crony, contrafagotto rophy.
Rational Review
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The first step was to position a cursor at the entry of the pipette.
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The borosilicate pipettes were coated with dental wax to record macroscopic currents and with sylgard to record single-channel currents.
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Because if the sperm count is low, what we can do is do a procedure called Ixy, which is where you pick up single sperm in a micropipette and actually inject it into the egg to get fertilization.
CNN Transcript Mar 14, 2004
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(A) Maximal projection of a Texas Red-labelled astrocyte syncytium after patching a single astrocyte with a Texas Red-containing pipette in an EC slice.
PLoS Biology: New Articles
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The human egg recipient was enucleated with a micropipette.
How pro-lifers view the PBA issue
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The slide and a 20-x 40-mm coverslip were prewarmed to 37° and an aliquot of the agar suspension of cells was pipetted onto the prewarmed slide between the taped regions.
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After 24 h the contents of the container were stirred and 25 ml was pipetted into test tubes and freeze-dried.
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For intracellular measurements the probe is commonly a glass micropipette that has been back-filled with a salt-solution and has a tip diameter suitable for insertion into a plant cell.
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Take up a little hydrochloric acid in a pipette and put a few drops of it into a very dilute solution of nitrate of mercury, and you will obtain rings of mercurial chloride that will, in their descent, take on the same whirling motion that characterizes the aureolas of phosphureted hydrogen.
Scientific American Supplement, No. 611, September 17, 1887
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The cell suspension was pipetted onto cold glass slides; specimens were air-dried and stained with Giemsa (Sigma).
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Method 1 is suitable for small diameter pipettes whereas method 2 is for larger ones.
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I set up my experiment today, in a 96-well plate (here's one similar to what I was using, if you don't know the beasts), and spent a good chunk of the afternoon pipetting in a few microliters of this and a few microliters of that.
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The Australian pair, with established reputations as musicians and composers, use a chemistry set of test tubes, Bunsen burner, pipettes, beakers and bowls rather than an orchestra for their musical performance.
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I wonder how long I'd have to pipet before that would happen.
Bustin' a Move
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From the concentrated algal suspension, nearly 300 individual cells were isolated in the laboratory within six hours of capture, using micropipettes and a binocular microscope.
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For the test, he imbibed 250 ml of warm water or plain (unsugared) tea, after which fresh blood was drawn from his finger with an Autoclix mini-lancet and a pipette.
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Carefully tighten the clamp until it firmly supports the pipette in a vertical position.
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Two micropipettes were positioned in a dual entry chamber mounted on the microscope stage.
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Literature data about the critical value of the relative area increase vary between 3% and 5%, both measured with micropipettes.
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Preparation of solutions, buffers used in electrophysiology lab and routine duties (such as pipette calibration, filling of nitrogen dewer).
Naturejobs - All Jobs
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Biophysical Journal describes how his team carefully controlled water removal during glassification by releasing single tiny droplets of water-dissolved protein into the organic solvent decanol with a micropipette.
Domain-b Brand Dossier
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If the spectra are dominated by fluctuation of the motor charges, subtraction of two spectra obtained at different pipette potentials should have a similar relaxation time.
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Subsequently 50 L of 50°C warm vesicle solution was pipetted into the cell.
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I'd go to grab my pipet gun and, lo and behold, it's not where I left it, you know, on my bench.
Archive 2008-10-01
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Liquid black ink, 25 c.c. Tincture of iodine 1 c.c. Allow the mixture to stand 24 hours, centrifugalise thoroughly, pipette off the supernatant liquid to a clean bottle and then add a crystal of thymol or one drop of formalin as a preservative.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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After incubation, 5 ml of RPMI media were added to the cell flasks and then the cell solution was pipetted to a 50-ml vial.
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Patch pipettes were filled with the same 100 MM KCl solution.
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A schematically shows how a glass micropipette is brought in contact with the cell, and B, using a higher magnification, a part of the cell membrane, with ion channels, in close contact with the tip of the pipette.
Physiology or Medicine 1991 - Press Release
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I begged one of my non-Mango colleagues to take my place as a surrogate Mango researcher; I even offered to let him keep the multi-channel pipet at his bench.
Colonel Candid with the Dagger in the Conference Room
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The precipitate formed is allowed to settle, 50 c.c. of the supernatant solution is removed with a pipette and transferred to a beaker; 50 c.c. of decinormal nitric acid and some water is added with sufficient cochineal tincture.
Scientific American Supplement, No. 324, March 18, 1882
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The deflection of the micropipette was measured and translated into force.
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His was made from glass by a glass-blower who made laboratory equipment, test-tubes, pipettes and so on.
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The hypophase was removed with a Pasteur pipette and the aqueous phase re-extracted with chloroform until it was colourless.
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Sampling occurs when the extract is pipetted into the PCR mix and again when the primers and polymerase bind the template DNA in the PCR.
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Following this spin, the ethanol was pipetted off, and each DNA pellet was washed with 70% ethanol and dried in a vacuum centrifuge.
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The inner diameter was measured by inserting a probe pipette with a known diameter profile (characterized by scanning electron microscopy).
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The cDNA samples were pipetted into separate tubes and stored at - 80°C.
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Identified neurons were excited or inhibited when these amino acids were squirted onto them from very fine micropipettes.
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The hard glass pipes of ‘Borosil’ make, used as pipettes and burettes in chemical laboratories, are his raw material.
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Now Forgacs and a company called Sciperio have developed a device with printing heads that extrude clumps of cells mechanically so that they emerge one by one from a micropipette.
Boing Boing: April 9, 2006 - April 15, 2006 Archives
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The oocytes were mechanically released from large antral follicles and freed of cumulus cells by gently pipetting through a mouth-operated micropipette.
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With a sterile 10 c.c. graduated pipette transfer 1 c.c. citrated blood from the Erlenmeyer flask to each tube of liquefied agar.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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A constricted glass pipette was used to mechanically separate cells from each other.
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Briefly, two borosilicate glass capillaries of 1.0 and 1.5 mm in diameter were twisted together and pulled to a micropipette.
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For Eppendorf's stab at the boy band, pipette-appeal genre, see the video here.
Science
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Application of pressure through the recording pipette accelerated the positive shift and removal of pressure simply slowed the shift.
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Mechanical stimulation of both cultivars by probing with blunt micropipettes without penetrating the epidermal cells resulted in regular depolarizations each time the root was probed.
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Therefore, the surfaces of borosilicate glass test tubes, pipettes, and so forth, have an affinity for endotoxin, and its removal is essential before they are used for the dilution of endotoxin.
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A small glass instrument, called a pipette, comes with each machine.
Agriculture for Beginners Revised Edition
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Advance has been along two lines, markedly in relation to insect-pollination, one of which has culminated in the hypogynous epipetalous bicarpellate forms with dorsiventral often large and loosely arranged flowers such as occur in Scrophulariaceae, and the other in the epigynous bicarpellate small-flowered families of which the Compositae represent the most elaborate type.
Encyclopaedia Britannica, 11th Edition, Volume 2, Part 1, Slice 1
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I normally use glass pipets when adding chloroform for a particular protocol, but I didn't pay attention and used a plastic pipet instead .. once ... it dissolved before my eyes like it was being melted, then, after a moment it clicked --- stupid it's an organic solvent and this is plastic!!!!
A Candid Chemist, She is Not
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The kit contains test tubes, pipettes, funnels and other lab materials necessary for multiple experiments and activities.
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As lactation continues it turns pink because it's very high in iron, and then later in lactation the milk is so thick it's almost like the thickest cream and you cannot even get it up in a pipette.
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A stock solution of 10-4 M pIX in methanol was pipetted into small containers and allowed to evaporate.
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The micropipette diameter was also measured, and the value multiplied by a factor of 0.92.
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This material was transferred to 1.5 ml Ependorff tubes with pipettes.
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If a volatile alkaloid, add a few pieces of calcium chloride to ethereal solution to absorb the water; draw off the ethereal solution with a pipette, allow it to evaporate, and test the residue for the alkaloids, conine and nicotine.
Aids to Forensic Medicine and Toxicology
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The mixture was gently pipetted and then centrifuged at 1000 rpm for 10 minutes.
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The top aqueous phase was pipetted into a fresh tube, and RNA was precipitated with 0.5 ml isopropanol.
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Using the pipettes from the kit you can extract a few drops of heady floral (jasmine and ylang ylang), mix it with balsamic (bergamot, jasmine and vanilla) and floral floral (jasmine, mandarin and violet).
Mary Orlin: A Scentsible Valentine's Day
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This process can be hastened by gentle pipetting of the egg masses through a wide bore glass mouth pipette.
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The Drink Detective comes with three test strips and a pipette, which is like a tiny eye dropper.
CTV BritishColumbiaHome
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I just checked the size of a 5-µl water drop from a pipet.
Archive 2007-08-01
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Advance has been along two lines, markedly in relation to insect-pollination, one of which has culminated in the hypogynous epipetalous bicarpellate forms with dorsiventral often large and loosely arranged flowers such as occur in Scrophulariaceae, and the other in the epigynous bicarpellate small-flowered families of which the Compositae represent the most elaborate type.
Encyclopaedia Britannica, 11th Edition, Volume 2, Part 1, Slice 1
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Once homogenised, the sample can be pulled down to the tip of the V between thumb and forefinger, the homogenate can then be extracted using either a pasteur pipette, or by cutting the bag to a sufficient height to enable use of a standard pipette.
BioSpace.com Featured News and Stories
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Glass micropipettes of desired diameters were prepared with a vertical pipette puller and a microforge as described elsewhere.
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Cells were ruptured by pipetting the resuspension 30 times through a Gilson 200 l tip.
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Bio-Consumables: "Despite their inherent hydrophobicity, the materials from which products such as pipettes, petri dishes and microplates are manufactured do retain residual amounts of liquid â€" resulting in inaccurate test results and the waste of costly reagents.
CoolBusinessIdeas.com
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DNA was dialysed by inserting the DNA sample with a pipette into dialysis tubing and securing the ends with clips.
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The chambers were assembled and collagen/cell suspension was pipetted inside the polycarbonate sheath.
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He bought equipment such as refurbished micropipettes — a staple in any molecular biology lab — and a used centrifuge on eBay.
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Pipettes were pulled from thin-walled borosilicate glass and had a resistance between 1.5 and 2.5 Mil.
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Lambda pipettes are used to transfer very small liquid volumes down to 1 microliter.
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Remove the solution of gelatine from the interior of the celloidin case with a pipette.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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( "Bluthendiagramme, II., page 482) the longer stamens may be either epipetalous or episepalous in this family.
More Letters of Charles Darwin — Volume 2
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The intact nuclei were incubated for 15 min after which they were gently pierced with two fire-polished glass micropipettes.
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But props on the attempted bribery with the multi-channel pipet!
Colonel Candid with the Dagger in the Conference Room
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Adult thymi were cut into small pieces and majority of thymocytes were released by gentle agitation using a glass pasture pipette.
PLoS ONE Alerts: New Articles
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Then, a small glass pipette was positioned near the cell.
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They hold the egg with a micropipette, suck up one of the sperm with a microscopic needle, somehow having cut the tail off, and inject the sperm head into the egg.
A New Cosmic Variant
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We used micropipette manipulation for forming and subsequently breaking the adhesive contact through a stepwise micromechanical procedure.
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Carefully tighten the clamp until it firmly supports the pipette in a vertical position.
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They were then dispersed into single cells by pipetting with Pasteur pipettes.
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In previous experiments probing stress relaxation, red cells or resealed ghosts were aspirated into micropipettes.
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Therefore, micropipette aspiration deforms the cell with a mixture of elastic and plastic deformations.
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Latex beads pipetted onto the surface of adherent aggregates attach to the membrane and are internalized.
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Aliquots of 5 l of sperm suspension were pipetted onto clean, dry glass slides.
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They developed a thin glass micropipette (a thousandths of a millimeter in diameter) as a recording electrode.
Physiology or Medicine 1991 - Press Release
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Carefully tighten the clamp until it firmly supports the pipette in a vertical position.
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It is composed of three parts, all made of glass; the mixing bottle, Fig. 34; the Pipette, Fig. 35; and the burette, Fig 36.
The Cultivation of The Native Grape, and Manufacture of American Wines
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Next, they took an unfertilized egg from a second sheep and removed its DNA, carefully sucking it out with a thin glass tube called a pipette.
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How To Make a Neuron Grow title Single Cell Electroporation in vivo within the Intact Developing Brain description How to perform single cell electroporation, using a micropipette to introduce DNA into a single neuron in tadpole brains.
How To Make a Neuron Grow
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Carefully tighten the clamp until it firmly supports the pipette in a vertical position.
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Electrophysiological measurements of brain activity play an important role in understanding signal propagation through individual neurons and neuronal networks, but existing technologies are relatively crude: Micropipette electrodes poked into cells are invasive and harmful, and microfabricated electrode arrays are too bulky to detect activity at the level of individual axons and dendrites, the neuronal projections responsible for electrical signal propagation and interneuron communication.
Wired
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Aliquots of each blood sample were pipetted into 2 separate Teflon [R] digestion tubes.
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The pipette tip was placed 5-10 m above the dish surface.
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For high-accuracy chemical analysis and research work, a volumetric transfer pipette is preferred.
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Before each aliquot was pipetted, the cell suspension was swirled to ensure that the cells remained evenly dispersed.
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In a Harvard Medical School corridor on a rainy Saturday afternoon, a handful of graduate students are emptying boxes of scientific equipment into the hallway to take inventory: microcentrifuge tubes, radiation counters, micropipetters, Erlenmeyer flasks.
NPR Topics: News
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This included a separate area for pre-PCR manipulations, dedicated air-displacement pipettes, barrier tips, and aliquoted stocks.
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Our device permits a more convenient control of both parameters than optical tweezers or micropipettes.
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On another micropipette, they put a red blood cell on which an antigen - technically known as a peptide-major histocompatibility complex - has been placed.
PhysOrg.com - latest science and technology news stories
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The pipette is simply a passive device that enables the recording, whereas a planar electrode offers the opportunity to further integrate devices on chip.
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H kingston 1 gb memory revenuer calibrated to lusciousness pipette, hereupon simultaneity, enforceable faeces, rtm counterdemonstrator, disgraceful crony, contrafagotto rophy.
Rational Review
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Preventive maintenance must be performed on all equipment related to the urinalysis including analyzers, pipettors, refrigerators, microscopes and refractometers.
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Each sample was stirred, mixed by pipeting up and down until suspended and transferred to a borosilicate glass test tube and mixed with 10 ml distilled water.
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It has developed technology that is used in extremely low volume pipettes and instruments that dispense minute droplets of liquid.
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Preparation of patch pipettes and electrophysiological instruments were the same as described above.
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(A) Maximal projection of a Texas Red-labelled astrocyte syncytium after patching a single astrocyte with a Texas Red-containing pipette in an EC slice.
PLoS Biology: New Articles
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Becki is the only one in the band who doesn't play an instrument but her role is no less important, as she choreographs the dance moves which are a major part of the Pipettes' package.
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The hypophase was removed with a Pasteur pipette and the aqueous phase re-extracted with chloroform until it was colourless.
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Though the expression incorporated the separate effects of driving pressure and minimum capillary radius on neutrophil transit time, it was based on studies using blunt-ended micropipettes.
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The caudal medulla was exposed to insert a microinjection pipette into the XII nucleus.
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Students modeled the spread of disease within a population by sharing simulated "body fluids" using pipettes.
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The pipette microchamber introduces a mechanical constraint around the annulus where the pipette tip contacts the plasma membrane.
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The precipitate was spread homogeneously over the entire surface of the tubed medium using a serologic pipette.
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I set up my experiment today, in a 96-well plate (here's one similar to what I was using, if you don't know the beasts), and spent a good chunk of the afternoon pipetting in a few microliters of this and a few microliters of that.
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By ‘them’ we mean creations inspired by such everyday objects as bottle gourds, pipettes and pumpkins, not to mention hourglasses and Japanese dining tables.
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One describes cells as having a solid cortex and viscous liquid core and has been used to model the aspiration of cells with no or little cytoskeleton (such as red blood cells or unattached leukocytes) into micropipettes.
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Its because I work in a research lab and we use instruments called "pipettes" day in and day out to transfer liquids around.
Archive 2005-07-01
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We assign this trapping to strong dielectrophoresis due to the high electric field and electric field gradient in the pipette tip.
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These last featured a plastic pipette at the end of the skewer that was filled with a wild basil pesto dressing to suck when you'd finished the prawns.
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These were shaken for 1 hour, then the solutions were centrifuged and the clear supernatant was pipetted into vials.
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Collect a quantity of pleuritic effusion, if such is present, in a pipette for further examination later.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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-- The saline diluting fluid _must always_ be taken into the pipette first, otherwise if the serum contains a very large amount of agglutinin the traces of this serum added to the saline solution may be sufficient to entirely vitiate the subsequent observations -- whilst if more than one sample of serum is diluted from the same saline solution serious errors may be introduced into the experiments.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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The organic phase was transferred with a disposable pipette, and the aqueous phase was extracted twice more with the same solvents.
