How To Use Phosphatase In A Sentence
-
The salubrinal / cantharidin-sensitive phosphatase activity nevertheless seemed to be required to maintain viability in the face of extended proteasome inhibition and when this activity was blocked, cell viability was reduced or lost.
PLoS ONE Alerts: New Articles
-
In addition, phosphatases specific for the phosphorylated forms of the glycolytic enzymes increase in activity under the influence of insulin.
-
The plates were then overlaid with an alkaline phosphatase assay solution.
-
Some RGV genes and their functions have been identified, including deoxyuridine triphosphatase (dUTPase), thymidine kinase (TK),
PLoS ONE Alerts: New Articles
-
Liver function tests, or LFTs these include alanine transaminase [ALT] and aspartate transaminase [AST], prothrombin time, albumin, bilirubin, and alkaline phosphatase
DR. SANJIV CHOPRA’S LIVER BOOK
-
Alkaline phosphatase-labeled complexes react with the substrate creating a chemical reaction and a source of energy to excite the dioxetane substrate.
-
Rabbit anti-human immunoglobulin conjugated with alkaline phosphatase was used to detect the bound antibody.
-
Protein phosphatases comprise a broad group of enzymes that catalyse the hydrolytic cleavage of phosphate from proteins.
-
Sections were incubated overnight at 70°C with 1 µg/ml of the digoxigenin-labeled probes, and binding detected by overnight incubation with alkaline phosphatase-conjugated anti-digoxigenin antibody (1: 3500, Roche), which was visualised with NBT (Nitro blue tetrazolium chloride)/BCIP (5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt; 1: 50, Roche) or Fast Red (Roche) for fluorescence.
PLoS ONE Alerts: New Articles
-
ANTIDIABETES Improving the secretion of insulin by repairing damaged pancreatic beta-cells; promoting the absorption of glucose in the liver; inhibiting the release of glucose from the liver by increasing hexokinase activity; reducing glucose-6-phosphatase activity
Forever Young
-
In this manner, the glycogen catabolizing phosphorylase is regulated by two enzymes working in opposing directions in a reversible process, one kinase and one phosphatase.
Physiology or Medicine 1992 - Press Release
-
Elevated alkaline phosphatase is associated with liver disease and with both obstructive jaundice and intrahepatic jaundice.
-
Ammonium molybdate was added to all reactions to inhibit activity from non-specific phosphatases.
-
The levels of serum parathyroid hormone(PTH) and bone-specific alkaline phosphatase(BAP) were detected respectively before and after the treatment.
-
Laboratory findings included elevated levels of alkaline phosphatase and lactate dehydrogenase.
-
Blood tests showed raised levels of aspartate transaminase, alkaline phosphatase and bilirubin, confirming liver damage.
-
To assess ERK phosphorylation, animals were exposed to a light pulse (20 or 100lx) or no pulse (0lx) at CT15, and then perfused with 4% paraformaldehyde in PBS including 1 mM sodium orthovanadate and 0.2 mM phenylmethyl sulfonyl fluoride to inhibit phosphatase activity, at an appropriate time after light stimulation (7.5 min for the 20lx stimulus and 15 min for the 100lx stimulus).
PLoS ONE Alerts: New Articles
-
It works by intervention of a phosphorylation reaction - it inactivates the phosphatase calcineurin.
Physiology or Medicine 1992 - Press Release
-
Patients presenting with obstructive jaundice show increases in serum bilirubin, alkaline phosphatase, and glutamyl transferase.
-
The granules contain several enzymes: alkaline phosphatase, collagenase, and lysozyme.
-
The ability to mine P is attributed to the development of specialized ‘proteoid’ or ‘cluster’ roots that exude large quantities of carboxylates and phosphatases.
-
The second operon encodes a phosphatase, an epimerase and the thiamine pyrophosphokinase (TPK).
Elites TV
-
The bound microparticle complexes are then incubated with antidetection hapten alkaline phosphatase conjugate, which binds to the detection haptens.
-
I have tried to describe your field of research and elegant discoveries in your studies of reversible protein phosphorylation, going back to the initial detection of the activation mechanism of phosphorylase, and continuing with protein phosphatases.
The Nobel Prize in Physiology or Medicine 1992 - Presentation Speech
-
SHP-2, a cytoplasmic SH2 domain containing protein tyrosine phosphatase is involved in the signaling pathways of a variety of growth factors and cytokines.
-
Liver function tests routinely combine markers of function (albumin and bilirubin) with markers of liver damage (alanine transaminase, alkaline phosphatase, and glutamyl transferase).
-
Slide 11: ENZYMES THAT REFLECT CHOLESTASIS • Alkaline phosphatase and 5 'nuleotidase: found near the bile canalicular membrane of hepatocytes • Gamma Glutamyl Transpeptidase: Endoplamic reticulum and in bile duct epithelial cells lacks specificity
Recently Uploaded Slideshows
-
We used an alkaline phosphatase anti-alkaline phosphatase staining method, which is generally more sensitive than immunoperoxidase methods.
-
Neither cantharidin nor okadaic acid can inhibit activities of this phosphatase.
PLoS ONE Alerts: New Articles
-
Hepatic dysfunction, checking allergies in cycles (ast and/or alt), dicumarol bilirubin, and/or alert phosphatase, has withheld essentially reported with augmentin.
Wii-volution
-
Acid phosphatase was initially contained within a continuous membrane in a sedimentable particle.
-
Antiinflammatory activity: A phosphate buffered saline extract of the roots of P. zrylanica stabilised red blood cells subjected to both heat - and hypotonic-induced lyses, A biphasic response and a reduction in the enzymatic activities of alkaline and acid phosphatases were observed and adenosine triphosphate activity was stimulated in liver homogenates of formaldehyde-induced arthritic rats.
Find Me A Cure
-
Hydrolases are enzymes that split molecules with the addition of water, for example, amylase, lipase, and alkaline phosphatase.
-
Provenge, an investigational vaccine for the treatment of prostate cancer, works by stimulating the immune system to recognize a protein known as the prostatic acid phosphatase or PAP, which is found in about 95% of all prostate cancer cells and attacks the cancer cells.
TradingMarkets
-
Mutation of the inositol polyphosphate 5-phosphatase OCRL1 causes the X-linked disorder oculocerebrorenal syndrome of Lowe, characterized by defects in the brain, kidneys, and eyes.
Journal of Biological Chemistry current issue
-
Elevated levels of an enzyme called lactate dehydrogenase LDH also may be a clue to the presence of liver disease, especially if your alkaline phosphatase level is elevated too.
DR. SANJIV CHOPRA’S LIVER BOOK
-
Alkaline phosphatase is not at all, or only in small amounts present in root exudates of vascular plants.
-
One potential mechanism for these differences is the differential expression of PTP-PEST, a tyrosine phosphatase known to dephosphorylate Pyk2 in T cells
PLoS ONE Alerts: New Articles
-
AEC substrate-chromogen was purchased from Dako and cocktail protease and phosphatase solutions for protein harvest from Roche
PLoS ONE Alerts: New Articles
-
The AST test is often done to determine liver function in conjunction with other tests, including alanine aminotransferase (ALT) and alkaline phosphatase (ALP).
You Being Beautiful
-
Human alkaline phosphatase isoenzymes have attracted much interest because of their clinical implications.
-
They stained strongly positive for alkaline phosphatase, were karyotypically normal and, most importantly, made splendid chimeras.
The 2007 Nobel Prize in Physiology or Medicine - Advanced Information
-
M. tuberculosis Serine/Threonine phosphatase (Mstp, Rv0018c) is shown to dephosphorylate both PknJ and mtPykA, thus proving that autophosphorylation and transphosphorylation of PknJ and mtPykA are reversibly regulated.
PLoS ONE Alerts: New Articles
-
To illustrate this point, consider a system containing two converting enzymes E 1 and E 2 (for instance, a protein kinase and a phosphatase) that interconvert substrates W and W *.
-
In contrast, signal amplitude is found most sensitive to the phosphatase reactions at the ERK level.
-
The roles of protein tyrosine phosphatase SHP 2 in breast cancer(MCF 7) cell adhesion and migration were investigated.
-
(PHLPP1 and PHLPP2), phosphatases that selectively dephosphorylate the HM of Akt, resulting in decreased kinase activity
PLoS ONE Alerts: New Articles
-
These observations suggest that the mutated tyrosine phosphatases are tumor suppressor genes, regulating cellular pathways that may be amenable to therapeutic intervention.
-
PP2A inhibition prevents p107 dephosphorylation and FGF-induced cell cycle arrest The regulation of Rb protein family phosphorylation by cyclin / cdk kinases is fairly well understood, but much less is known about the phosphatases that dephosphorylate Rbs during the normal cell cycle or in response to exogenous signals.
PLoS ONE Alerts: New Articles
-
Immunohistochemical detection by, for example, alkaline phosphatase or peroxidase has been utilized in some papers.
-
Reaction product of acid phosphatase is localized primarily in the small vacuoles, and occasionally in the dictyosome cisternae and vesicles of the golgi body.
-
Double immunostainings with anti-LMP2/anti-Olig2 and with anti-PA28-α/anti-Olig2 were performed following the previous procedure but employing as second chromogen Perma\Blue AP Chromogen (Diagnostic Biosystems, Pleasonton, CA, USA) for alkaline phosphatase.
PLoS ONE Alerts: New Articles
-
This notion is also supported by the observation that substitution of salubrinal with subtoxic concentrations of the phosphatase inhibitor cantharidin induced a comparable increase in PSI-mediated cytotoxicity, whereas the PP2B / calcineurin inhibitor cypermethrin proved to be ineffective.
PLoS ONE Alerts: New Articles
-
Elevated alkaline phosphatase is associated with liver disease and with both obstructive jaundice and intrahepatic jaundice.
-
Phosphatases, acting as the "off switch," control the activation of protein kinases.
-
An increase was seen in all 7 rabbits transfected with alkaline phosphatase plasmid.
-
Rabbit anti-human immunoglobulin conjugated with alkaline phosphatase was used to detect the bound antibody.
-
A phosphatase inhibitor, sodium vanadate, reduced the effect of alkaline phosphatase and the p48 band remained undetectable.
-
Preliminary experiments showed that congelation in these conditions did not affect the phosphatase activity.
-
A phosphatase inhibitor, sodium vanadate, reduced the effect of alkaline phosphatase.
-
There were no significant differences in the serum lactate, pyruvate, or alkaline phosphatase concentrations between the treatment groups.
-
To assess ERK phosphorylation, animals were exposed to a light pulse (20 or 100lx) or no pulse (0lx) at CT15, and then perfused with 4% paraformaldehyde in PBS including 1 mM sodium orthovanadate and 0.2 mM phenylmethyl sulfonyl fluoride to inhibit phosphatase activity, at an appropriate time after light stimulation (7.5 min for the 20lx stimulus and 15 min for the 100lx stimulus).
PLoS ONE Alerts: New Articles
-
This phosphoprotein is regulated post-translationally by reversible phosphorylation catalysed by a protein kinase and a microcystin-sensitive serine/threonine protein phosphatase.
-
It reduced levels of glycolytic enzymes such as hexokinase, phosphoglucoisomerase and aldolase levels, which are increased in hepatoma-bearing rats, and increased levels of gluconeogenic enzymes such as glucose. 6-phosphatase and fructose-I
Find Me A Cure
-
Sections were incubated overnight at 70°C with 1 µg/ml of the digoxigenin-labeled probes, and binding detected by overnight incubation with alkaline phosphatase-conjugated anti-digoxigenin antibody (1: 3500, Roche), which was visualised with NBT (Nitro blue tetrazolium chloride)/BCIP (5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt; 1: 50, Roche) or Fast Red (Roche) for fluorescence.
PLoS ONE Alerts: New Articles
-
These observations suggest that the mutated tyrosine phosphatases are tumor suppressor genes, regulating cellular pathways that may be amenable to therapeutic intervention.
