How To Use Oocyte In A Sentence
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Oocyte cryopreservation is being sold as such a policy, but it is no such thing.
Times, Sunday Times
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These studies show that compromised oocyte and early embryo mitochondrial metabolism, resulting from excessive nutrient exposure prior to and during conception, may underlie poor reproductive outcomes frequently reported in obese women.
Maternal Excess Weight Stresses Oozytes | Dr. Sharma's Obesity Notes
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Before starting the experiments, oocytes were equilibrated for at least 20 min in substrate-free bathing medium to obtain a stable volume measurement.
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Temporary heat stress near the time of estrus and ovulation can reduce oocyte quality by altering meiotic processes, directly affecting conceptus quality and survival.
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He neglects to mention that metaphase I oocyte chromosome alignment is mechanistically different from mitosis the chromosomes are physically tethered to each other, and that metaphase II, which is mechanistically similar to mitosis, does have abnormalities.
Wells vs tiny flies - The Panda's Thumb
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Here, we are trying to address Sgo1's roles in chromosome separation by exogenous protein overexpression to enhance, or by RNAi to suppress Sgo1 function during two meioses of mouse oocytes.
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Using mouse oocytes, 80 percent of eggs that had been vitrified became fertilized with ICSI, with a live birth rate of about 30 percent, comparable to conventional IVF when eggs are not frozen.
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Absolute majority oocytes consist in mammalian ovary in preantral follicles , antral follicles a small portion.
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Furthermore, they may cause premature depletion of the follicle pool by induction of follicular atresia or oocyte apoptosis and, thereby, lead to reduced fertility.
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Methods Dual-electrode voltage clamp technique was used to record membrane currents from follicular oocytes, and defolliculated oocytes that had been injected with alien RNA and incubated.
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Mutants in all three meiotic genes display diakinesis oocytes containing 12 individual DAPI - stained bodies corresponding to achiasmate univalent chromosomes.
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This was first recognized more than a half century ago in cytological studies of lampbrush chromosomes in amphibian oocytes and polytene chromosomes in insects.
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The immediate task was to identify the gene or genes involved in oocyte maturation.
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Oocytes were isolated and singularized by collagenase.
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Publisher: Putman Media, Inc. Influence of the formamidine pesticide chlordimeform on ovulation in the female hamster: dissociable shifts in the luteinizing hormone surge and oocyte release (SuDoc EP 1.23/6: 600/J-94/040) by U.S. Environmental Protection Agency
OpEdNews - Quicklink: US Drinking Water and Watersheds Widely Contaminated by Hormone Disrupting Pesticide, Atrazine
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In both the cases, oocytes were exposed to the cytostatic chemical, nocodazole, from the time of hormonally stimulated resumption of meiosis.
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The abnormal FSHR contained a deleted sequence of protein called exon 2 that is an important part of the protein that binds the FSH; FSHR with the exon 2 deletion was only detected in women younger than 35 who had a poor response to FSH and yielded less than four oocytes in a follicle stimulating cycle.
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The reason for aneuploid embryos are mostly aneuploid oocytes immature egg cells, as aneuploid sperm are relatively rare with an estimated frequency of about 2 per cent, while aneuploid oocytes are quite common with an estimated frequency of more than 20 per cent," he says.
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Absolute majority oocytes consist in mammalian ovary in preantral follicles , antral follicles a small portion.
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Isochronal tail current amplitudes were normalized to the maximum amplitude obtained from that oocyte and plotted versus test potential.
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We confirm recent studies that osk transport in the nucleus can be modeled by what we term diffusive transport whilst in the nurse cell and the oocyte it occurs via super-diffusive transport.
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HMGN3a transcripts were present at similar levels in bovine matured oocytes and 2-4-cell embryos but at higher levels in 8-16-cell embryos, morulae and blastocysts.
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Title: Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies
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Just keep the oocyte in a medium that is lacking in calcium.
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Hypotheses were developed to explain why stem-line oogonia are restricted to the anterior region of the ovary, how the branching pattern of the cystocyte cluster arises, or why only one of the two pro-oocytes becomes the oocyte.
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Each primary oocyte is contained in a cluster of supporting cells.
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Many studies atresia caused by granulosa cell apoptosis is associated with the subsequent oocyte developmental competence.
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Reproductive Sciences at Yale, said: "When a woman undergoes in vitro fertilisation, she receives medication called Follicle Stimulating Hormone to produce more than one oocyte, which is the normal production each month.
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So you can effect cell fusion without activating the oocytes if you do this in a calcium-free medium.
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A prepared sample containing around one to two million motile sperm is required for adequate oocyte fertilisation with in vitro fertilisation.
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In Dolly's case, her nuclear DNA was the same as the donor animal; other of her genetic materials came from the mitochondria in the cytoplasm of the enucleated oocyte.
Cloning
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For long-term incubation, oocytes were cultured in MEM with 20\% foetal calf serum in a
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The transparent, 100-m-diameter oocyte is fertilized and undergoes rapid mitotic cleavage cycles.
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In some experiments, oocytes were microinjected with salt solution immediately before recordings.
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Slide 7: SCNT • Starts with removal of polar body and chromosomes from an oocyte • Enucleated oocyte • Donor cell then inserted into perivitelline space of enucleated oocyte
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In common snook, the germinal epithelium is active throughout the year, producing follicles where the oocyte is in arrested meiosis, diplotene of the first meiotic prophase, and the germinal epithelium is always discontinuous.
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The oocytes were mechanically released from large antral follicles and freed of cumulus cells by gently pipetting through a mouth-operated micropipette.
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Time, Voltage and ionic concentration dependent of rectification of h - erg expressed in xenopus oocytes.
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And most of us have probably ingested meat and dairy products from livestock cloned by natural reproduction (monozygotic siblings), mechanical embryo-splitting or even nuclear transfer from an embryonic donor cell into an enucleated oocyte.
The Cloned Cow Has Left the Barn
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In this study, the aim is to optimize the systems of IVM, IVF of oocytes and IVC of embryos in ovine and further promote embryo biology engineer development.
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So you can effect cell fusion without activating the oocytes if you do this in a calcium-free medium.
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Using an established mouse model of maternal diet-induced obesity and live cell dynamic fluorescence imaging techniques, the researchers demonstrated that maternal obesity prior to conception is associated with altered mitochondria in mouse oocytes (eggs) and zygotes.
Maternal Excess Weight Stresses Oozytes | Dr. Sharma's Obesity Notes
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Oocytes ingested from contaminated soil or other sources excyst in the duodenum, and released sporozoites invade the intestine.
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Astaxanthin could stimulate the ovarian development of E. sinensis and accumulate protein in oocyte and vitellin.
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A preincubated, capacitated sperm suspension was gently added to the freshly ovulated oocytes to give a final motile sperm concentration of 1 x [10.sup.6] / ml.
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For instance, the migration of the male pronucleus within the animal oocyte after fertilization, together with movements of the female pronucleus, are essential events for nuclear fusion.
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SCNT involves transferring the nucleus of a somatic cell (any body cell other than a sperm or egg cell) into an enucleated oocyte, i.e. an oocyte from which the nucleus and thus most of the DNA has been removed.
Cloning
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An alternative approach is cryopreservation of slices or biopsies of ovarian tissue, which contain many thousands of immature oocytes.
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Specification of the primordial germ cells (PGCs) takes place via different strategies across animal phyla; either specified early in embryogenesis by the inheritance of maternal determinants in the cytoplasm of the oocyte ( 'preformation') or selected later in embryonic development from undifferentiated precursors by a localized inductive signal ( 'epigenesis').
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Our studies on oocytes ultrastructure, in which experimental materials came from Wapiti , was just one of the blanks.
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He reports that while Xkid deficient frog cells have PEF defects, frog oocytes have normal metaphase I.
Wells vs tiny flies - The Panda's Thumb
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A woman who persuades a friend to donate oocytes will not get them herself.
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Any bias in the segregation of one or the other of a pair of homologous chromosomes or chromatids between the oocyte and the polar body can have a dramatic effect on the genetic makeup of a population.
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Many of these immotile spermatids are dislodged from the spermatheca and swept away as oocytes parade through the reproductive tract.
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Generally, this reflects a decline in the number of oocytes in the ovary (decreased ovarian reserve).
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The technique is called gamete-targeted lentiviral transgenesis - essentially, inserting genes into feline oocytes eggs before sperm fertilization.
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• These factors prevent primary oocytes from maturing beyond the prophase of the 1st meiotic division.
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It produces the female egg cells necessary for reproduction, called the ova or oocytes.
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Oocytes and sperm are haploid, with one set of chromosomes, whereas somatic cells are diploid, with two chromosomal sets.
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For introduction of spermatid mitochondria, in vitro fertilization was carried out by microinjection of spermatids into unfertilized oocytes as described previously.
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A unique look into laboratory techniques for egg freezing, also known as oocyte cyropreservation.
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The oocyte is then allowed to develop to the blastocyst stage in vitro.
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And most of us have probably ingested meat and dairy products from livestock cloned by natural reproduction (monozygotic siblings), mechanical embryo-splitting or even nuclear transfer from an embryonic donor cell into an enucleated oocyte.
The Cloned Cow Has Left the Barn
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It might also have a place in other assisted reproductive techniques such as oocyte transfer and gamete intrafallopian transfer (combining eggs and sperm outside the body, then placing them in the fallopian tubes to achieve fertilization), he said.
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Title: Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies
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One of the major changes that occurs during the maturation of oocytes is the accumulation of yolk protein, or vitellin.
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Absolute majority oocytes consist in mammalian ovary in preantral follicles , antral follicles a small portion.
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Blood products, sera, sperm, oocytes and embryos are not included.
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Even for women who ovulate, there is no information about oocyte quality and consequent embryo quality after fertilisation.
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A Quantitative analysis of the activation and inactivation kinetics of HERG expressed in xenopus oocytes.
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[3] The ovum is really the fully mature egg ready for fecundation; before maturity it should not be called ovum but oöcyte; and in advanced treatises it is so referred to.
Woman Her Sex and Love Life
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Objective To assess the effect of repeated gonadotropic stimulations on the developmental potential and growth differentiation factor-9 (GDF-9) expression of mouse oocytes.
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The pilot study shows that in vitro grown and matured oocytes from preantral follicles that progress to metaphase II possess euploid numbers of chromosomes.
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Each oocyte was microinjected with 40 nl of cRNA solution.
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The technique is called gamete-targeted lentiviral transgenesis — essentially, inserting genes into feline oocytes eggs before sperm fertilization.
Glow-in-the-dark cats against AIDS, other diseases
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And most of us have probably ingested meat and dairy products from livestock cloned by natural reproduction (monozygotic siblings), mechanical embryo-splitting or even nuclear transfer from an embryonic donor cell into an enucleated oocyte.
The Cloned Cow Has Left the Barn
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As can be seen in Fig 4A and Fig B, the developing egg chambers in these ovarioles contain nurse cells with polyploid nuclei and a cell at the posterior that may correspond to the oocyte.
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The study also identified a gene that regulates oocyte quality.
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In most crustaceans, the production of primary oocytes derived from oogonia continues throughout adult life.
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In mammals, signals from the gonads determine whether the germ cells develop into oocytes or sperm.
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As semen quality reduces, the proportion of oocytes fertilised by in vitro insemination decreases.
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Cytotoxicity of GCV on cell cultures was determined using Cell Proliferation Kit I (MTT) (Roche, Mississauga, ON) according to the manufacturer's instructions. pAlb-vTK was digested with BstXI and HindIII to yield a 5134 bp minigene containing the murine Alb p/e with the downstream vTK coding region which was microinjected into fertilized oocytes prior to their transfer into the oviducts of pseudopregnant FVB/N mice using standard procedures
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Egg freezing is more technically known as oocyte cryopreservation, a technology by which a woman's eggs are surgically removed from her ovaries and frozen until she is ready to use them.
Why I Froze My Eggs
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All 40 chromosomes of mouse oocytes are telocentric, so homologs form bivalents in meiosis I while sister chromatids form univalents in meiosis II
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These cells, about 40 m in diameter and termed primary oocytes, are enclosed within a single layer of squamous cells, forming a primordial follicle.
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Women with ovulatory dysfunction secondary to ovarian failure or poor ovarian reserve should consider obtaining oocytes from a donor source.
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Immature human eggs, or oocytes, sit in suspended animation in the ovaries from the time they are formed in the female fetus, and complete the process of meiosis to form mature egg cells only just prior to ovulation.
Delayed Childbearing
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However, in young women this comes at a price - many women lose ovarian function because oocytes or their support cells are damaged by the treatment.
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What inspired him to take on this challenge was his research on how hormones control critical ovarian functions in mice, such as oocyte maturation and ovulation.
British IVF pioneer Robert Edwards wins Nobel prize for medicine
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Since female mice are fertile for more than a year, their ovaries had to be generating new oocytes, the scientists reasoned.
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The oocytes were then extracted in a 37°C microtubule-stabilizing buffer containing non-ionic detergent and glycerol.
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Preactivating donor cells and enucleated oocytes with ionomycin has no significant enhancive effect on the cleavage of c...
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Our results reveal that LGN is cortically polarized in mouse oocytes and is critical for meiotic spindle organization.
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In sexual reproduction, spermatocytes develop from the transformation of choanocytes, and oocytes arise from archeocytes.
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Mammalian females have a fixed number of oocytes at birth, which decreases dramatically with advancing age due to follicular atresia.
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• In late fetal period primary oogonia enlarged to form primary oocytes.
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