How To Use Oligonucleotide In A Sentence
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The latter is generally restricted to oligonucleotides but offers higher specificity.
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Progeny phage that produced clear plaques in the presence of IPTG and X-gal were analyzed by a differential oligonucleotide hybridization technique, as reported.
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Spiller DG, Giles RV, Grzybowski J, Tidd DM, Clark RE (1998) Improving the intracellular delivery and molecular efficacy of antisense oligonucleotides in chronic myeloid leukemia cells: a comparison of streptolysin-O permeabilization, electroporation, and lipophilic conjugation.
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First, two non-complementary strands of phosphate backbone modified oligonucleotides (PM oligo-DNA) were loaded on the surface of the 15 and 25 nm Au nanoparticles, respectively.
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One important application is gene therapy, where it is necessary to deliver a gene or a synthetic oligonucleotide into the cell.
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Thimmulappa RK, Mai KH, Srisuma S et al. "Identification of Nrf2-regulated genes induced by the chemopreventive agent sulforaphane by oligonucleotide microarray.
Dr. Richard Palmquist: Green Veterinary Medicine: Veggies for Pets
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For single bulges in RNA oligonucleotides, the conformation usually depends on the nature of the flanking base pairs.
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The peptides can be linked to a large protein or an oligonucleotide, which may then be carried through the cell membrane.
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The quaternary nitrogen atom in lycobetaine plays an important role in the formation of hydrogen bonds between the compound and the oligonucleotide.
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The bands containing labeled oligonucleotide were identified by visual inspection and ultraviolet shadowing.
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The unmodified polynucleotides may be DNA, RNA or synthesized oligonucleotides.
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The platinated oligonucleotides were repurified by ion-exchange fast protein liquid chromatography.
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This problem would be enhanced using arrays composed of long oligonucleotides since only RNAs whose protected regions overlap with the oligo sequence would be identified.
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The latter is conjugated to primer oligonucleotides that are pre-annealed to a complementary circular oligonucleotide.
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However, all the advances of ribozymology notwithstanding, the prospects of a bona fide ribozyme replicase remain dim as the ribozymes designed for that purposes are capable, at best, of the addition of ~10 nucleotides to a oligonucleotide primer, at a very slow rate and with fidelity at least an order magnitude below that required for the replication of relatively long RNA molecules [28,29].
Again, there is absolutely no teleology involved
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The following oligonucleotide pairs were annealed to form double-stranded DNA and coinjected into worms along with the DNA components of the lacI / lacO system.
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Point mutations were created by oligonucleotide-mediated site-directed mutagenesis and confirmed by DNA sequencing.
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The GAACGACTCTACAATGTTGTtGTAGAtACAGAAGTTACTG GTAAAAAG oligonucleotide was used to mutagenize full-length SMC2 cDNA (a gift from K. Yokomori), which was subsequently inserted into NheI and XhoI sites of pcDNA3. 1, to produce the pcDNA3. 1/SMC2-2 complementation plasmid, resistant to RNAi with RNA duplex #2, due to silent mismatch mutation at the siRNA-corresponding site.
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Genes were amplified from genomic yeast DNA using specific oligonucleotides and the polymerase chain reaction.
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The world's lingering,the end is still no match for the forget the bitter oligonucleotide soup.
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Instead I've ended up working 10-11ish hour days trying to play catch up to get the World's Most Uncooperative Plasmid to ligate my oligonucleotides and getting the yeast ready to do the shuffle.
Archive 2008-05-01
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The drugs in the latter complexes are covalently connected by a peptide linker, whereas those associated with the oligonucleotide duplexes are chemically independent species.
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The unmodified polynucleotides may be DNA, RNA or synthesized oligonucleotides.
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The proper orientation and the sequence of the cloned oligonucleotides were checked by nucleotide sequencing.
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Standard cytogenetic analysis including preparation of karyotypes, metaphase and interphase based fluorescence in situ hybridization (FISH), high resolution single nucleotide polymorphism oligonucleotide based array (SNP array) analysis and mutation analysis of the INI1/SMARCB1 gene are available.
Cancer Cytogenetics Lab
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These masks define exposure sites on the chip's surface, where a light-directed synthesis process constructs the oligonucleotides combinatorially.
The Scientist
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In the first, DNA is spotted onto a glass slide; in the second, oligonucleotides of 15-30 nucleic acid base pairs are synthesised on to a silica slide by a process known as photolithography.
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Probes are typically fairly short sequences of DNA nucleotides, called oligonucleotides, made synthetically.
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The unzipping direction (order of nucleotides unzipped) was controlled by the selection of oligonucleotides.
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The oligonucleotide is chemically modified for in vivo stability and specific placental uptake.
The Scientist
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Creation and purification of the double-labeled oligonucleotide is synthetically demanding.
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Researchers demonstrate efficacy of antisense therapy for spinal muscular atrophy disease spinal muscular atrophy (SMA) might soon be treated with tiny, chemically modified pieces of RNA called antisense oligonucleotides (ASOs).
WN.com - Articles related to Length of biological marker linked to cancer
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On single-stranded oligonucleotides, all the guanines are oxidized to the same extent.
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Li S, Waters R (1996) Nucleotide level detection of cyclobutane pyrimidine dimers using oligonucleotides and magnetic beads to facilitate labelling of DNA fragments incised at the dimers and chemical sequencing reference ladders.
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DNA chips are highly miniaturized devices containing an orderly, matrix-like arrangement of synthesized oligonucleotides or pure DNA fragments.
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In apoptosis, DNA is degraded into oligonucleotides with a few hundreds of basepairs that subsequently diffuse out of the nucleus.
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In vitro studies on novel DNA structures have mainly involved synthetic oligonucleotides based on naturally occurring sequences.
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We have been examining the potential use of oligonucleotides to direct nucleotide exchanges in episomal and chromosomal genes.
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In each mutagenesis reaction, the functionality of the resistance genes is reversed using a pair of "on/ off" oligonucleotides.
The Scientist
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Liposomal formulations of genetic drugs such as antisense oligonucleotides and plasmid DNA are difficult to achieve.
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Knocking out XIAP with siRNA or antisense oligonucleotides restores chemosensitivity to a variety of malignant cell lines
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The purity of the double-stranded oligonucleotides was confirmed by polyacrylamide gel electrophoresis.
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The world's lingering,the end is still no match for the forget the bitter oligonucleotide soup.
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This was followed minutes later by the injection of the oligonucleotide complementary to the DNA strand immobilized on sphere S2.
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The world's lingering,the end is still no match for the forget the bitter oligonucleotide soup.
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These studies demonstrate that pre-existing cytosine methylation on oligonucleotides…
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Close to the sphere surface, the oligonucleotide interacts with the evanescent field of the WGM, which extends about a wavelength into the buffer solution.
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The oligonucleotides were annealed by heating the solution of self-complementary oligonucleotides to 94°C using a heat block, and then cooling it to 30°C over the course of 2 h. Annealed DNA was stored at - 80°C until use.
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The world's lingering,the end is still no match for the forget the bitter oligonucleotide soup.
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Intermolecular tetrameric complexes are usually only formed by short oligonucleotides that contain single tracts of contiguous guanines.
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This problem would be enhanced using arrays composed of long oligonucleotides since only RNAs whose protected regions overlap with the oligo sequence would be identified.
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In this work, the molar concentrations of the oligonucleotide duplexes are related to the double-stranded molecules 15-bp long.
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These oligonucleotides were attached to Dynal magnetic beads coated with streptavidin according to the manufacturer's instructions.
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The oligonucleotide is chemically modified for in vivo stability and specific placental uptake.
The Scientist
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As shown in Fig. 3, both oligonucleotides were able to bind F9 proteins and to produce retarded complexes.
