How To Use Nuclease In A Sentence
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Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
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Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
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This mutant contains a single amino acid substitution in the nuclease catalytic center of RecB.
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The rapid rise of the first fluorescence maximum puts a strict upper bound on the timescale of magnesium binding to the exonuclease.
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Barnase is an extracellular ribonuclease produced by Bacillus amyloliquefaciens.
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Let us remember that they are concerned with a DNA endonuclease, so I had some experience dealing with an enzyme that cut nucleic acids.
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These DNA-binding proteins include polymerases, helicases, nucleases, isomerases, ligases, histones, and others.
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Chromatin was digested with 0, 20, and 30 units/ml of micrococcal nuclease.
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Coding-sequence nucleotide substitution is an important mechanism driving the differential adaptive evolution of species and has been studied most notably in hemoglobin, ribonuclease, and lysozyme.
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DNA polymerase, Pol I, extends the DNA from the free 3'-OH using its exonuclease activity to replace the nucleotide of the damaged base, as well as a few downstream, followed by sealing of the new DNA strand by DNA ligase.
Another Protozoan and Front-Loading
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To further study the basis for these changes in chromatin organization, we used micrococcal nuclease digestion to determine the arrangement of nucleosomes in this region.
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Intact nucleosomal structure was verified by micrococcal nuclease digestion.
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These blocked forks could potentially be processed into DNA breaks by an endonuclease.
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Such restrictions in the conformational freedom imposed by denaturants have been suggested through analyses of x-ray B-factors for lysozyme, ribonuclease A, and dihydrofolate reductase.
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Double-stranded RNA is cut into short pieces (siRNA) by the endonuclease Dicer.
Advanced Information: The 2006 Nobel Prize in Physiology or Medicine
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In most cell types DNA cleavage occurs after irreversible activation of endonucleases.
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The unfolding pathway was compared with that of the parent enzyme ribonuclease A (RNase A), and a model was devised to assess the importance of the dissociation in the unfolding.
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The present invention proposes exonuclease III digesting double stranded nucleic acid molecule coated in microporous plate and containing special marker to detect transcription factor protein.
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Anfinsen had shown, with the enzyme ribonuclease, that the information for a protein assuming a specific three-dimensional structure is inherent in its amino-acid sequence, and this discovery was the starting point for studies of the mechanism of protein folding, one of the major areas of present-day biochemical research.
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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Plants then synthesize antimicrobial substances, including the protein chitinase, which degrades the cell walls of fungi, and enzymes called nucleases, which break apart the ribonucleic acid of viruses.
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Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
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Restriction map A map of a segment of DNA showing the cleavage sites of restriction endonucleases and their physical distance apart, usually measured in base pairs.
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Phage gene 46 encodes one subunit of a putative exonuclease that is thought to be directly involved in recombination and recombinational repair.
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Then a company separated the gene for human deoxyribonuclease, which chops up the protein but does not cause allergic reactions.
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Has discussed in initially the frog egg the ribonuclease active constituent.
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Meselson's laboratory at Harvard University to study a DNA endonuclease involved in the replication and recombination of T4
Sidney Altman - Autobiography
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By 1950, the list of products included deoxyribonuclease, erythrogenic toxins, fibrinolysin, haemolysin, hyaluronidase, leucocidin, lipoproteinase, proteinase, ribonuclease, streptodornase, and streptokinase.
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In recent years, Anfinsen has devoted himself primarily to comprehensive investigations of an extracellular nuclease of
Christian Anfinsen - Biography
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Total DNA was prepared from leaves of barley after 7 days of germination and restricted with appropriate endonucleases.
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This study shows that in a competition between the two enzymes, DNA ligase trumps the flap endonuclease in capturing bubbles and creating expanded tracts.
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Restriction map A map of a segment of DNA showing the cleavage sites of restriction endonucleases and their physical distance apart, usually measured in base pairs.
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Church, “The cleavage site of the restriction endonuclease Ava II,” Nucleic Acids Research 5 1978, 2313–2319.
The $1,000 Genome
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OncoMouse (cancer mouse) for research, through genetic redesign. restriction endonucleases, which are able to cut DNA at specific sites.
Citizendium, the Citizens' Compendium - Recent changes [en]
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Subsequently, it has been shown that RISC contains at least one member of the argonaute protein family, which is likely to act as an endonuclease and cut the mRNA (nowadays often referred to as the Slicer function).
Advanced Information: The 2006 Nobel Prize in Physiology or Medicine
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Orn was shown by in vitro analysis to be an exonuclease specific for RNA molecules shorter than five nucleotides in length.
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Hamilton Smith telling me about the restriction endonuclease he had discovered in Hemophilus influenzae aroused my interest in the possibility of using restriction enzymes to dissect the genomes of DNA tumor viruses.
Daniel Nathans - Autobiography
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Role for a bidentate ribonuclease in the initiation step of RNA interference.
Advanced Information: The 2006 Nobel Prize in Physiology or Medicine
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In Belgium, as late as 1945, Gratia's group observed that nucleases (RNase and DNase) had no effect on four tested bacteriophages of various origins.
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CP4-EPSPS gene was amplified from 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of PCR products.
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The pdb-codes of the proteins are as follows: endonuclease III - 2ABK, dihydrodipicolinate synthase - 1DHP, blue copper oxydase CuoE - 1KV7, threonine dehydratase - 1TDJ, penicillin amidase - 1PNK.
PLoS ONE Alerts: New Articles
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The second involves the joining of DNA ends through the pairing of short complementary sequences, as happens with complementary overhanging DNA ends produced by endonucleases.
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A given exonuclease hydrolyzes DNA at a fixed rate, but there exist a distribution of conformations and a distribution of hydrolysis rates.
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When both sets of fingers are bound, the cleavage domain can dimerize to form an active nuclease and cleave the DNA at the sites indicated by carats.
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Such hydrolytic enzymes as cystein proteinases, serine proteinases, and nucleases are highly induced during xylogenesis.
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A number of catabolic enzymes were known: nucleases, nucleotidases, nucleosidases, deaminases (for guanine, adenine, adenosine, and adenylic acid), xanthine oxidase and uricase.
Nobel Lecture The Purine Path To Chemotherapy
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Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification.
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Secondly, the preamplified DNA fragments were digested by a restriction endonuclease to form sticky ends, which were then ligated to a designed DNA adapter by ligase.
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The present invention proposes exonuclease III digesting double stranded nucleic acid molecule coated in microporous plate and containing special marker to detect transcription factor protein.
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Mass spectra of proteins such as ribonuclease A(RNase A) and lysozyme were obtained with substantially enhanced abundant peaks of low charge states.
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Primers and endonucleases are used in the cloning of the various constructs.
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Such hydrolytic enzymes as cystein proteinases, serine proteinases, and nucleases are highly induced during xylogenesis.
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Deoxyribonuclease, or recombinant human deoxyribonuclease 1 (rhDNase or DNase), is an experimental drug used to treat cystic fibrosis, an inherited lung disease.
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For characterization of the damage process, the plant material was analysed for total chlorophyll and for the enzyme activity of deoxyribonuclease in three replicates as described previously.
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This was an exciting period in the molecular biology of adenovirus with the discoveries (a) that only one specific fragment of the genome, the E1 region, was responsible for oncogenic transformation; (b) that restriction endonuclease length polymorphism could be utilized to generate genetic maps; (c) the mapping of specific genes on the viral genome; and (d) generation of a viral map of sequences expressed as stable RNAs.
Phillip A. Sharp - Autobiography
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The spt6-140 mutation alters transcription and chromatin in our inverted repeats, as determined by Northern and micrococcal nuclease sensitivity analyses, respectively.
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The endonuclease digestion map of pWTA was consistent with the fragment length in anticipation.
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The binding mechanism of psoralens to a double-strand DNA has been well investigated by chemical and enzymatic methods using restriction enzymes and exonuclease.
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After a restriction endonuclease cleavage, two expected smaller fragments were observed.
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Samples were digested with the appropriate restriction endonuclease and were size fractionated by electrophoresis through 0.9% agarose gels.
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AP endonuclease recognizes this site and nicks the damaged DNA on the 5 'side (upstream) of the AP site creating a free 3'-OH.
Another Protozoan and Front-Loading
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Ribonuclease was the first enzyme for which the complete amino acid sequence was determined thanks to contributions from Anfinsen and from Moore and Stein.
Nobel Prize in Chemistry 1972 - Presentation Speech
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The chemistry of water activated by a magnesium ion is central to the function of the DNA repair proteins, apurinic/apyrimidic endonuclease 1 (Ape1) and polymerase A (Pol A).
Signs of the Times
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After all, in nature we can and do observe bacteria using restriction endonucleases and ligases to cut DNA.
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Endonuclease-sensitive regions of human spermatozoal chromatin are highly enriched in promoter and CTCF binding sequences is to be published in the forthcoming issue of Genome Research.
Innovations-report
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DNA was digested to mononucleotides with micrococcal nuclease and spleen phosphodiesterase at 37°C for 3.5 h.
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Cleavage of DNA by R1 restriction endonuclease generates cohesive ends," Proc.
Asilomar and Recombinant DNA
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These DNA-binding proteins include polymerases, helicases, nucleases, isomerases, ligases, histones, and others.
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The core technology in TILLING is screening mutant library by using single-strand endonuclease.
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Ulf, I, and others generated the first restriction endonuclease maps of this virus, and Dr.S. Jane Flint and I began to analyze the regions of the genome expressed as mRNAs in both productively infected cells and in adenovirus transformed cells.
Phillip A. Sharp - Autobiography
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The most advanced of Sangamo's drugs uses a zinc-finger nuclease to treat diabetic neuropathy, a common complication of diabetes that causes nerve damage and pain.
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The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.
