How To Use Microscope In A Sentence
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The machine then applies a thin, even layer of cells to a microscope slide for examination.
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… Others can only be identified with a microscope, such as the majority of protozoa [single-celled animals], certain blood filariae [small larvae of pathogen parasites in the blood vessels], and the trichinae.
Modern Science in the Bible
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If any are found, a biopsy then takes place, where small samples of body tissue are examined under the microscope for abnormal cells.
The Sun
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The microscope capacitates small objects to be observed.
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The sludge from the bottom of the swamp that the dredge hauls up dripping and oozing at least has substance: you can dry it out, look at it through a microscope, describe it, or flush it down the toilet.
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He is the most talented young English player around and as such he is under the microscope.
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The morphology of peripheral blood cells of adult Silurus meridionalis has been studied with both electron and light microscope, and, in addition, glycogen and peroxidase in cells were detected.
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The microscope contains a few hundred dollars-worth of electronic bits and bobs.
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The microscope condenser collects this light.
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Society on a small discovery which he had made by the aid of a "wretched microscope" to the effect that the so-called ova of Flustra were really larvæ and had the power of independent action by means of cilia.
Alfred Russel Wallace: Letters and Reminiscences, Vol. 1
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In 1882, scientists using microscopes discovered the chromosomes separately.
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The paper shows that positrons can see defects better than either optical or electron beam microscopes.
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We calculated a mean of three measures for each size parameter that were done with a binocular microscope with x60 magnification.
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All but toy microscopes are parfocal and parcentered.
MAKE Magazine
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These were filled with mature, refringent spores, as observed under the microscope (not shown).
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With the aid of lacker varnish and skilful painting, paper made excellent trunks, tobacco bags, cigar cases, saddles, telescope cases, the frames of microscopes; and we even saw and used excellent water-proof coats made of simple paper, which did keep out the rain, and were as supple as the best macintosh ... ..
The Art of Travel Shifts and Contrivances Available in Wild Countries
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Diameters at 1-4 mm behind the root apex were measured using a stereo microscope with an eyepiece graticule.
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This shaped beam profile is imaged through the telescope system onto the back focal plane of the microscope objective.
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The President feels that the entire world is looking through a microscope at his brain cells. margo franssen
Beat 360° 9/30/09
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This laboratory is equipped with a new intravital microscope system and is interested in the role of intracellular signaling molecules in leukocyte recruitment.
Naturejobs - All Jobs
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Slides were mounted using glycerol - gelatin and photographed at a magnification of x79 using a Zeiss photomicroscope.
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Pollen traps constructed from petroleum jelly on microscope slides were attached to vertical wooden laths facing the direction of the prevailing wind.
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By the dawn of the Scientific Revolution, researchers equipped with microscopes founded modern chemistry - and dismissed alchemy as hocus-pocus.
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The right wing of each male was mounted on a microscope slide and photographed with a digital camera through a stereoscopic microscope at a magnification of 40x.
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A measure of the magnification of an optical instrument, such as a microscope or telescope.
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Under the microscope, a stem section is needed to demonstrate the small, incrassate epidermal cells.
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This microscope is equipped with a field-emission gun, thus it provides an atomic resolution in imaging and good energy resolution in spectroscopy.
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These research microscopes often have binocular eyepieces, relying upon a series of prisms to split the image so that it may be viewed with both eyes.
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This microscope magnifies an object 100000 times.
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Under the microscope the slide was found to be covered with a mass of raphides.
Scientific American Supplement, No. 829, November 21, 1891
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Among the viruses that are put under the microscope are the adenoviruses, bunyaviruses, retroviruses and the coronaviruses (the genus behind SARS outbreak).
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The chromosomes were stained with acetic orcein and visualized with a light microscope.
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Manson reasoned that certain suctorial insects were the agencies through which blood was most commonly removed from the circulation and he ventured the guess that this change in the parasite that may be seen taking place on the slide under the microscope, normally takes place in the stomach of some insect that sucks man's blood.
Insects and Diseases A Popular Account of the Way in Which Insects may Spread or Cause some of our Common Diseases
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The epidermis was spread out on a glass plate beneath a low-power binocular microscope and cut into pieces of the required size using a combination of razor and scalpel blades.
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Visual inspection of the quality of the metal pattern was carried out using a 20x lens on an upright light microscope.
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At other times, it seems, Drebbel made and used single-lens microscopes of high magnification, comparable to Leeuwenhoek's.
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The distinct defects of yolk lyotropic liquid crystal (YLLC) during follicular atresia of Tilapia mossambica were widely found by electron microscope with freeze -etching.
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In fact, metrology is concerned with nothing less than finding a method of being able to control the constancy of the international prototype metre, the basis of the whole metric system, so accurately that not only will every change, however small, which could possibly occur in it be accurately measured, but also if the prototype were entirely lost, it could nevertheless be reproduced so exactly that no microscope could ever reveal any divergence from the original prototype.
Nobel Prize in Physics 1907 - Presentation Speech
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The microscope contains a few hundred dollars-worth of electronic bits and bobs.
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It is helpful to detect the responsible blood vessel with operating microscope or endoscope.
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Methods The CWDMs of Salmonella typhi were induced and compared with its parental type by agglutination test and electron microscope.
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It was high time, he said, that the artist acquaint himself with the fantastic images of the microscope.
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Under the microscope these tumors showed an increase in the interfascicular as well as perivascular fibers, but the nerve-fibers were not increased in size
Anomalies and Curiosities of Medicine
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The Universal C-mount Adapter was introduced at the request of a number of customers who wanted to their camera images to be parfocal and parcentral with the images seen in the microscope eyepieces "states Dr. Paul Martin, President of CRAIC Technologies.
ThomasNet News - Today's New Product News
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Scientific instruments such as telescopes, synchrotrons, and electron microscopes generate raw data streams that are archived for subsequent batch processing.
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Here we take a look through the microscope to see if they have progressed any further up the evolutionary tree.
The Sun
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Objective To evaluate the effect of dental operating microscope(DOM)and ultrasonic root canal technique(URA)in the management of calcified root-canal.
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Abnormalities in the cells can be seen quite clearly under a microscope.
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The researchers chemically bonded one end of the molecule to a bendable cantilever similar to the tip of an atomic-force microscope.
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The banding pattern of polytene chromosomes was examined under a Zeiss phase-contrast microscope.
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Although concerns are likely to continue to centre on infections, you don't need a microscope to see the biggest threats.
Times, Sunday Times
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They have these little statoliths or balance organs in the back of their head and we can section those and look under a microscope and have daily rings just like tree rings in a tree stump.
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When necessary, fragmented samples were picked with tweezers from a sieve under a binocular light microscope.
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Love looks with telescope; envy with microscope.
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Designed much like a compound microscope, the electron microscope uses a beam of electrons focused through magnetic lenses.
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You'd probably need a microscope to see it.
The Sun
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Viable and nonviable seeds can easily be distinguished using a binocular microscope, because the latter lack an embryo, whereas viable seeds contain an embryo.
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Images of dissected tendon taken under the light microscope show that fibrils can sustain sharp bends or kinks along their length.
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Frequently, examination of a fracture face with a low-power binocular microscope can reveal the type and cause of failure.
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We aimed the microscope at a likely looking target.
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Under ultra-violet light it glows and the DNA code can be read under a microscope.
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Based on J. Robert Oppenheimer's theories of quantum mechanics, as well as on Ruska's groundbreaking research, the field emission microscope allowed magnification up to two million times.
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Regardless of the implement used, the prosector will perform the best microdissection while looking through the eyepieces of the microscope.
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CRAIC Technologies Universal C-mount Adapter for Microscope Cameras CRAIC Technologies Universal C-mount Adapter is designed allow a camera or a spectrophotometer to be parfocal and parcentral with the eyepieces on your microscope.
Physicsworld.com: all content
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It seems unlikely its present owner will be putting it under an electron microscope.
Times, Sunday Times
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The coverslips were mounted onto glass microscope slides with the addition of 3 l of mounting medium.
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It is this industrial market that excites potential manufacturers of acoustic microscopes.
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The fixative solution was partially but not completely removed, the pellet was resuspended, dropped onto pre-cleaned microscope slides and dried for 24 h at room temperature.
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Examination of cells under the microscope to identify the presence of malignancy.
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Apoptotic phenotype of SPCA - 1 was observed by HE dying and electric microscope.
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The 1.0-carat stone was fashioned into a marquise and shows several tiny unidentified mineral inclusions only visible under the microscope.
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The microscope also has an autofocus mechanism.
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Processes of analogy have created coinages like petrodollar, psycho-warfare, microwave on such models as petrochemical, psychology, microscope.
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Improved variants of GFP and GFP-like proteins in synergy with high-resolution microscopes, computational techniques and powerful theoretical approaches are currently fuelling a scientific revolution focused on quantitative analyses of complex biological systems.
The Nobel Prize in Chemistry 2008 - Presentation Speech
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The most obvious way in which we can escape from the physical limitations of our eyes is to employ a microscope, magnifying glass, or optical telescope to improve magnification and resolution.
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Programmable movements on a given slide are possible using the Olympus microscope controller with appropriate software.
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He filled the time consumed by the staining process with the ritual of aiming the microscope's substage mirror out the window at a brilliant white cloud.
A Case Of Conscience
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The images of the stomata were taken with the microscope focused to the narrowest part lower down in the pore.
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The payday loans industry is facing a sixth official crackdown, with the regulator announcing new plans to put its debt collection methods under the microscope.
Times, Sunday Times
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There is not enough time in a day to sit with a microscope, zooming in on each tiny difference between the "overqualified" applicants.
Melanie Wallner: Watch Out Seniors, The Super Applicants Are Taking Over the World!
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The abdominal tissue is tunneled underneath the skin to the chest (pedicle flap), or surgically transplanted there utilizing a microscope (free flap).
Robert Tornambe, M.D.: Reconstructive Surgery: What are Your Options After Breast Cancer?
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A minor unknown mineral with leaf shape is determined as pyrophyllite using scanning electronic microscope.
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A range of clues like these can be identified very easily by looking at the shape of crystals in a scanning electron microscope.
EXTINCTION: Evolution and the End of Man
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Under the microscope it turns out to be a collection of prejudices masquerading as arguments and distortions dressed up to look like facts.
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Use of a dissecting microscope to examine the colony will reveal that the surface of the colony has a beaten copper appearance.
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Mr Edlestein, IBM Fellow and chief scientist of the self-assembly airgap project, said: We have managed to harness the kinds of processes we see in nature to make regular patterns – such as the layers of enamel on your teeth, or the shape of a seashell if you look under a microscope.
IBM’s Snowflakes Yield Faster Chips | Impact Lab
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The beetles' backs are covered with bumps - under a microscope, they resemble a landscape of peaks and valleys.
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I just need my microscope, a lamp and a box of small tools.
Times, Sunday Times
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We know that looking for abnormal cells down a microscope is not an exact science, and that is the problem.
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And, under the microscope, that food just became mold, fungi, and yeast fairly quickly.
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This simple approach recognises the fact that mycobacterial culture is not feasible in peripheral units but that light microscopes and trained microscopy staff are available.
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Leaves were sectioned on a sliding microtome and stained with safranin and Alcian Blue before being dehydrated through an alcohol series, taken through Histoclear and mounted onto microscope slides in Euparal.
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It is obviously desirable, before attempting to interpret the structures exhibited, under the microscope, to compare the fresh and uncalcined materials with those that have been more or less altered by heat.
Scientific American Supplement, No. 595, May 28, 1887
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From each plant one randomly chosen, fresh flower was dissected under a binocular microscope to separate the corolla, androecium and gynoecium.
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The sections were mounted, stained with toluidine blue, scanned and photographed under a microscope.
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Not content with the instruments he had, Monty built microscopes, a spectroscope and other pieces of equipment, including a micrometer with a powered eyepiece for better speed control.
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Although the snow on downhill and cross-country courses can look smooth on television, under the microscope snow crystals are jagged and have varying water contents.
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For these analyses, robotic equipment precisely places pieces of the pathogen's DNA in an array of infinitesimally small droplets on glass microscope slides.
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In a confocal microscope, light passes through a pinhole to eliminate photons from outside the image plane.
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Users can view labeled caspases under a fluorescence microscope or analyze them with a flow cytometer or fluorescence plate reader.
The Scientist
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The modern achromatic compound microscope was invented in 1878, and it was this instrument that added the extra dimension of the microscopic study of tissues to anatomical teaching.
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The mobile clinic's microscope is not state-of-the-art, but it was clear enough to see the hyperactive flagellated trichomonads scurrying around the field.
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Mr. Ellis observed by his microscope near the surface of all putrefying vegetable or animal matter, which is the mucor or mouldiness; the vegetation of which was amazingly quick so as to be almost seen, and soon became so large as to be visible to the naked eye.
Note I
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The researchers used a custom-built microscope with an iron-coated tip to manipulate cobalt atoms on a plate of manganese.
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It is a minute spheroid in which the best microscope will reveal nothing but a structureless sac, enclosing a glairy fluid, holding granules in suspension.
Essays
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He would have benefited from the microscope, telescope, barometer and pendulum clock.
Times, Sunday Times
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When viewed under a microscope, the liver is seen as a large network of units called hepatic lobules.
CreationWiki - Recent changes [en]
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The vegetative and propagative organs of Cymbidium sinense were studied by paraffin method and observed under scanning elect ron microscope.
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The changes of outer hair cells(OHC) were observed by scanning electron microscope.
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The CRAIC Technologies Universal C-mount Adapter is designed allow a camera or a spectrophotometer, such as the CRAIC Technologies QDI 302 (TM), to be parfocal and parcentral with the eyepieces on the microscope.
ThomasNet News - Today's New Product News
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This is then viewed under a microscope to look for the entamoeba histolytica parasite.
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An atomic force microscope operating at hypergravity for in situ measurement of cellular mechano-response.
SpaceRef Top Stories
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At last, humanity has a ready means of putting pathological liars and other disparate evildoers under the collective microscope of blogdom every time they try it again.
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Different rayon based carbon fiber precursors are studied by means of scanning electron microscope(SEM), Thermal Mechanical Tester and X-ray diffractometer.
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We calculated a mean of three measures for each size parameter that were done with a binocular microscope with x60 magnification.
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The areolae on the valve are usually too small to be distinguishable with the light microscope.
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Even a century later, had physicians made better use of their microscopes, they could hardly have overlooked such an easily found parasite as the itch mite, which is quite as easily detected as the cheese mite, pictured in Hooke's book.
A History of Science: in Five Volumes. Volume II: The Beginnings of Modern Science
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If any are found, a biopsy then takes place, where small samples of body tissue are examined under the microscope for abnormal cells.
The Sun
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Primitive streak stage embryos can also be manipulated using a dissecting microscope but the lower resolution makes such manipulations less precise.
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After three subsequent changes of fixative, metaphases were prepared by dropping the cell suspension onto labelled clean, moist microscope slides.
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In a third method the interaction between two large vesicles formed at the end of micropipettes and imaged by a light microscope is determined.
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De Graaf, working without the aid of a microscope, made the error of thinking that the fluid-filled follicles were the ova themselves.
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He also studied the orientation of purines and pyrimidines in tobacco mosaic virus and in nucleic acids, by measuring the ultraviolet dichroism of oriented specimens, and he studied, with the visible-light polarizing microscope, the arrangement of virus particles in crystals of TMV and measured dry mass in cells with interference microscopes.
Maurice Wilkins - Biography
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Here we have used the atomic force microscope to directly observe changes in the atomic lattice on a calcite seed crystal after the introduction of abalone shell proteins.
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Piezoelectrical elements are used to control the horizontal movement of the stylus in two perpend icular directions so that it scans the surface a long parallel lines - hence the name scanning microscope The vertical movement of the stylus is controlled and measured using another piozoelement.
Press Release: The 1986 Nobel Prize in Physics
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Following treatments for 24 hr with DMSO alone (control), Red-Br-nos, LPS, Red-Br-nos and LPS, cells were stained with 1 µg/ml acridine orange for 15 min, washed with PBS, and examined under a Zeiss fluorescence microscope using a 40X objective lens Proteins from THP-1 cells pellets were resolved by polyacrylamide gel-electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore).
PLoS ONE Alerts: New Articles
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His place was already under the microscope following a faltering Test career that has brought three centuries but five ducks.
The Sun
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He would have benefited from the microscope, telescope, barometer and pendulum clock.
Times, Sunday Times
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We peer down at the tiny worm wriggling under the lens of our microscope.
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Had Galen, Celsus, Hippocrates, and the other great scientists of old, known the use of the microscope, they would have made no such grave blunders as in the advocation of the theory that the arteries of the human body contain and carry air during life, instead of oxygenized blood only.
The Arena Volume 4, No. 23, October, 1891
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The cuticle of juvenile stages is thin and weakly sclerotized, and sternal and tergal plates are barely recognizable in the light microscope.
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[*] The power which the eye possesses of adaptation to near and distant objects, combining the uses of the microscope and the telescope, and the capacity of self-adjustment, preserving always a perfect achromatism and freedom from spherical aberration, have never been reached in nearly the same degree by art.
Religion and Chemistry
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The measurements are performed in vitro, with a DNA construction anchored between a glass microscope slide and a silica bead.
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The notation of section thickness on a microscope slide informs the observer of the approximate level of magnification most suitable for examination of the tissue section.
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He looks for fine cracks under a microscope and tests the amount of pressure it can withstand before tearing.
Smithsonian Mag
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The structure and function of PM were observed with quantitative cytochemistry, lectinaffinitive cytochemistry, transmission and scanning electron microscope and phagocytic test.
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* The leaf, examined with a microscope at the instant we drew it up from the water, did not present, it is true, those conglobate glands, or those opaque points, which the parts of fructification in the genera of ulva and fucus contain; but how often do we find seaweeds in such a state that we cannot yet distinguish any trace of seeds in their transparent parenchyma.
Travels to the Equinoctial Regions of America
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Shots were taken at 300dpi resolution through some sort of Nikon trinocular microscope.
Historiffic
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These very long, spiraled nascent bdellovibrio were proposed earlier from electron microscope images to be common in spirilla, which are long and coiled themselves.
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The slightest criticism generates an overwhelming impulse to impale myself on my microscope eyepieces.
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Visitors will be shown how to build their own microscope that will allow them to see the creatures' likeness to bears.
Times, Sunday Times
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From each plant one randomly chosen, fresh flower was dissected under a binocular microscope to separate the corolla, androecium and gynoecium.
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Under the microscope these tumors showed an increase in the interfascicular as well as perivascular fibers, but the nerve-fibers were not increased in size or number.
Anomalies and Curiosities of Medicine
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The structure of molecules can be seen under an electron microscope.
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Lastly, with respect to karyokinesis, although it is true that the microscope has in comparatively recent years displayed this apparently important distinction between unicellular and multicellular organisms, two considerations have here to be supplied.
Darwin, and After Darwin (Vol. 1 and 3, of 3) An Exposition of the Darwinian Theory and a Discussion of Post-Darwinian Questions
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I must get a peep through that microscope of his and see the pediculus which occupies
Complete Project Gutenberg Oliver Wendell Holmes, Sr. Works
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The sample is then sent to a laboratory to be looked at under a microscope for signs of cell change.
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The length and width of anthers were measured with a micrometer under a stereomicroscope.
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Micropalaeontology is the study of microfossils, a microfossil being any fossil that is best studied by means of a microscope.
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They had sextants, early microscopes, clocks, thermometers, and barometers.
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Based on scanning electron microscope images of the failed nanotube films, we attribute the ultimate failure to agglomerates in the film (point defects that act as stress concentrators).
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Linen cloth observed through a microscope which magnifies the threads to a coarseness of about forty to the inch gives us the exact appearance of the buckram, which is a heavy, strong cloth well adapted to large books, and which furnishes the most durable binding of all the book cloths.
The Building of a Book A Series of Practical Articles Written by Experts in the Various Departments of Book Making and Distributing
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At the center I helped develop the electron microscope which produced the snooperscope.
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This combination is carried in a screw-centering holder known as the substage below the stage of the microscope (Fig. 40 f), and must be accurately adjusted so that its optical axis coincides with that of the objective.
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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In the meantime, Phoenix grabbed another scoopful of soil and planned to deliver it to its microscope later this week.
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The sting of the nettle is a very curious and interesting object under the microscope.
Country Walks of a Naturalist with His Children
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The third leg was removed with a fine probe, mounted on a microscope slide, and examined with phase microscopy.
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Therefore, gemologists, appraisers, and jewelers routinely use simple tools - such as a jeweler's loupe or microscope - to reveal more about a stone.
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A microscope with a calibrated eyepiece micrometer was used to measure the spheroid diameter.
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Inc. Images of zircon in cross section, as imaged by cathodoluminescence detector on a scanning electron microscope.
NYT > Home Page
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DNA microarrays consisting of thousands of individual gene sequences can be printed in a high density format on a glass microscope slide or deposited on a miniature matrix by a photolithographic process.
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Anyway I then headed over to the UCL campus and turned up to the lecture room and panicked that there where undergraduates in there with microscopes and the like doing some petrology.
Snell-Pym » 2009 » April
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a high-powered microscope
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We determined DNA migration with an Olympus BMX60 microscope with a fluorescent beam source, measuring with a scaled ocular.
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His place was already under the microscope following a faltering Test career that has brought three centuries but five ducks.
The Sun
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The mucus trails were protected with coverslips and observed under a light microscope.
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Other achievements of Aepinus include improvements to the microscope, and his demonstration of the effects of parallax in the transit of a planet across the Sun's disk.
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The first was the electron microscope.
The Times Literary Supplement
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Of course, I know that an odour of peach-blossom in the air, resulting in death, could only be associated with some vaporous effluvium of cyanogen, or of hydrocyanic ( 'prussic') acid, or of both; and when I at last managed to examine some of the dust under the microscope, I was not therefore surprised to find, among the general mass of purplish ash, a number of bright-yellow particles, which could only be minute crystals of potassic ferrocyanide.
The Purple Cloud
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The problem of variable flattening of specimens during negative staining and slight variations in the electron microscope magnification complicates the interpretation of small variations in diameter.
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Looking at these samples with his microscope, Leeuwenhoek reported how in his own mouth: ‘I then most always saw, with great wonder, that in the said matter there were many very little living animalcules, very prettily a-moving.’
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By 1998 he had developed a suite of programs covering aspects of various forms of light microscope, some of which are used to teach medical students.
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The optical system of a refracting telescope is essentially the as that of a compound microscope.
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The word ` slide 'is of a punning nature, and in conjunction with the easy moveability of the microscope-barrel suggests a meaning akin to that of dreams of skating and sliding, which are usually sexual.
The Journal of Abnormal Psychology
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The microscope cannot find the animalcule which is less perfect for being little.
V. Essays. Compensation. 1841
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His eyes narrowed in thought as he pulled his head away from the second microscope.
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Viruses are extremely small things, so even normal microscopes are not powerful enough to allow us to visualise a virus, so one has to use an electron microscope which magnifies objects by 40,000 times or so.
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Leaf vernation was observed in at least ten buds per entity, manually dissected under a stereomicroscope.
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She had spent weeks hunched over a microscope looking at samples of sperm.
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Butterfly in a Box, intending to examine its gaudery with my _Microscope_,
Micrographia Some Physiological Descriptions of Minute Bodies Made by Magnifying Glasses with Observations and Inquiries Thereupon
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Small holes were made in the film for the microscope objective lens and the micropipette.
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He likened this to the chances that an out-of-focus microscope could be focused by small vs. large random adjustments.
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The samples were then prepared on concave microscope slides under coverslips and sealed with silicone grease.
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In a compound microscope, a series of lenses are used to focus, magnify, and refocus the image.
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Methods The left and right common iliac artery were perfused with the ABS acetone solution and the red canvas paint chloroform solution, respectively, and then dissected under stereomicroscope.
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The wing is resting on a glass microscope slide but is not in mounting media.
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If you look through a microscope at a vinyl record being cut, you'll see the etchings of the grooves being formed.
Times, Sunday Times
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Instead, the abundance of leaf hairs on leaf surfaces or veins was scored for each sample under a light microscope.
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Peels were put in slide frames and studied under a binocular microscope and drawn using a camera lucida.
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Aged pupae were washed, dried, and then attached to a microscope slide with double-stick tape.
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The slight asymmetry in both the radial and image axis direction indicates small aberrations in the microscope lens.
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Nevertheless the apparent trajectory of an element in the ultramicroscope is not a curve without tangents.
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Micromounters are looked upon as some sort of secret society that does furtive things under microscopes for inexplicable reasons.
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Measurements of tooth base dimensions were made using digital calipers and/or a measuring eyepiece and dissecting microscope.
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Currently, treatment is based on whether a patient's cancer is classified as alveolar or embryonal, which is determined by the appearance of a tumour sample under the microscope.
Medlogs - Recent stories
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You'd need an extremely powerful microscope to see something so small.
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To solve these problems, a method for quantification of stain conditions from microscopic images taken by multispectral microscope systems has been proposed.
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Love looks with telescope; envy with microscope.
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Electron microscopes are usually equipped with an analytical system that also allows for simultaneous elemental analysis of the sample's surface.
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At the far end of the room, hovering in some kind of antigravity unit, a man was peering into a microscope.
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