How To Use Incubate In A Sentence

  • Twenty microliters of each antibody was added to 100 L of blood, and the mixture was incubated at room temperature for 15 minutes.
  • Daudi B cells were incubated in RPMI culture medium containing 0, 100 or 1000 nM CXCL12, 50 µM cycloheximide, and washed briefly with 1 M NaCl, 50 mM glycine, pH 3. PLoS ONE Alerts: New Articles
  • In experiments that test the effect of actin, cells were incubated for 30 min before experiments in medium containing 20 M cytochalasin D to disrupt actin filaments.
  • Doctors "incubated" the girl and planned on taking her to the Staten Island University Hospital's burn unit, sources said. WN.com - Articles related to Boston weighs smoke-free public housing
  • The female builds the nest and incubates and broods alone, but both parents feed the chicks, which fledge within 14-16 days of hatching.
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  • The temperature at which the eggs of ectothermic vertebrates incubate can influence several phenotypic traits of offspring.
  • When most birds incubate eggs, the females often produce high levels of prolactin, a hormone involved in parental behavior.
  • More than a dozen large eggs were arrayed around the pool, close enough to incubate, but not so close as to cook the young. RISE OF A MERCHANT PRINCE: BOOK TWO OF THE SERPENTWAR SAGA
  • The startling result was a marked increase in the density of the fibrillar halo around the ganglia incubated in the presence of the tumoral fraction treated with snake venom. Nobel Lecture The Nerve Growth Factor: Thirty-Five Years Later
  • The cells were fixed and permeabilized with Cytofix/Cytoperm ™ buffer (BD BioScience) for 20 minutes at 4°C and incubated with allophycocyanin anti-IFN-γ or corresponding isotype controls (eBioscience) for 20 minutes on ice. PLoS ONE Alerts: New Articles
  • After 24 and 48 h, aliquots were transferred to 96-well plates and incubated for 3 h with a tetrazolium salt.
  • Like the eggs of birds, monotreme eggs are incubated and hatched outside the body of the mother.
  • The temperature at which the eggs of ectothermic vertebrates incubate can influence several phenotypic traits of offspring.
  • We collected clutches, incubated the eggs, and took blood samples from hatching young.
  • Following incubation with fluorescence-conjugated secondary antibody (at room temperature for 60 min) and rinses, slides were incubated with DAPI (1: 10,000, Sigma, St. Louis, MO) for 15 minutes to counterstain nuclei, mounted, and cover-slipped. PLoS ONE Alerts: New Articles
  • Slides were washed with PBS-Tween20 (0. 02%) and incubated for 30 min with Alexa Fluor 488-F (ab′) 2 goat anti-mouse IgG, Alexa Fluor 594 phalloidin (both Invitrogen) and DAPI. PLoS ONE Alerts: New Articles
  • More than a dozen large eggs were arrayed around the pool, close enough to incubate, but not so close as to cook the young. RISE OF A MERCHANT PRINCE: BOOK TWO OF THE SERPENTWAR SAGA
  • The body was "incubated" by the 1990 Trust, an organisation founded by Mr Jasper that has received more than £100,000 in City Hall funding. Archive 2008-03-01
  • Instead of using body heat to directly incubate eggs, megapodes passively incubate eggs.
  • The male feeds the female while she incubates, catching small mammals and birds and bringing them back to the nest.
  • The tubes were incubated at ambient temperature.
  • To determine the presence of yeast in cloacae, swabs were streaked onto Sabouraud's dextrose agar and incubated for 14 days.
  • Excess solution was removed and fresh degassed buffer solution was added to the sample and allowed to incubate for 30-60 min before imaging.
  • The virus can incubate for up to ten days after the initial infection.
  • Sections were first incubated in buffer containing 3-amino - 9-ethyl carbazole and subsequently for 2 h following the addition of peroxidase and either Put (for DAO) or Spd (for PAO).
  • Neutrophils from healthy volunteers were incubated with DFP (2.5 mM), and neutrophil elastase (NE) activity; hypodiploid DNA formation; caspases-3 and -8 processing and activity; exteriorization of phosphatidylserine (PS); oxidative burst activity and hydrogen peroxide release were measured. BioMed Central - Latest articles
  • In addition to allowing regeneration of syncytiotrophoblast and investigating chronic regulation of apoptosis and autophagy, another reason for us to incubate villous explants and cytotrophoblastic cells under normoxia or HR for 48 hours before administration of vitamins C and E is to precondition the trophoblast cells with our experimental settings. PLoS ONE Alerts: New Articles
  • Sand tiger sharks are ovoviviparous (incubate eggs internally and offspring are born live).
  • Peter Haas, director of AIDG (the organization that "incubated" the Xela Teco workshop) says, Boing Boing
  • Smooth-billed anis, a crotophagid bird of Central and South America, engage in a system of social pairs sharing a single nest and competing for access to the incubated clutch of eggs.
  • Neutrophils from healthy volunteers were incubated with DFP (2.5 mM), and neutrophil elastase (NE) activity; hypodiploid DNA formation; caspases-3 and -8 processing and activity; exteriorization of phosphatidylserine (PS); oxidative burst activity and hydrogen peroxide release were measured. BioMed Central - Latest articles
  • The female builds the nest, incubates the eggs, and feeds the young on her own.
  • It was difficult to use the word reformist to describe Mousavi, because Iran’s political landscape had turned upside down during his absence, and little was known about what ideas and resolve may have incubated in the meantime. Let the Swords Encircle Me
  • The time needed for the eggs to incubate is nine or ten days.
  • Fresh pullet eggs (White Leghorn Strain) were incubated to obtain the primitive streak stage of development.
  • It would therefore take a single female 40-50 days to lay the maximum clutch that could be incubated.
  • Only if we notice that a bird is unable to incubate the egg is it then put in an incubator.
  • The period for which the flies were kept at 29° and the temperature at which the embryos were laid and incubated are indicated above the dot blots.
  • All adults at the nest incubated eggs and brooded the naked chicks.
  • The virus can incubate for up to ten days after the initial infection.
  • Slides were incubated with 1:16 diluted patient's sera and stained with fluorescein conjugated rabbit antihuman IgG antibodies.
  • In a related experiment, articular cartilage was incubated for two days with the enzyme elastase, which specifically digests the collagen fibers.
  • Diploid conidia were incubated on solid complete medium containing 60 g/ml benomyl for 2 days and transferred to complete medium without benomyl for 2 weeks.
  • The Petri dishes with embryos were incubated at 30°C in the dark.
  • While the Israeli and Palestinian territories are ever more disconnected from one another - whether walls of concrete, walls of hate, walls of psychosis incubated in the vacant lots of empty childhoods, or even literary festivals celebrating Israeli and Palestinian writers at the same time yet completely without acknowledgment of the other - we remain insistent on our island-like existence, instead of accepting we are each promontories and peninsulas. Qanta Ahmed, MD: The Adventures of Itamar Marcus and the Hamas Bunny: Palestine at Play
  • Yet, 12 years ago, when Gibson went through a low in his life, the idea of this work began to incubate in him.
  • The inset in Fig.3B shows a picture of stem segments incubated for the respective time on either 10 M IAA or 10 M IBA.
  • Laying-stage incubation presents a paradox for hatching synchrony: our data show that not all embryos are incubated for the same length of time, so some mechanisms must exist to synchronize hatch.
  • The cultures were incubated at 37 deg C in the controlled microaerophilic atmosphere described earlier or three days.
  • The male brings food and incubates the eggs when the female leaves the nest to eat.
  • Each female lays a single egg, which the male bird incubates; the chicks remain in their nests for eight to ten months before they are fully fledged.
  • Sometimes they were East Germanic, and that was a grief, taking, as it were, the bloom from the guess that had made him great; and again they were West Germanic, and that was awful, the hallucination ending in a mortal struggle with the feather bed under which German science is incubated, and passing off with an anguished The Collectors
  • (DWA), incubated for 5 d at 15 C until primary sporidia developed, then transferred to room temperature for analysis of ballistospore (secondary sporidia) discharge. PLoS ONE Alerts: New Articles
  • Females built the nest and incubated alone, but both parents fed nestlings and fledglings.
  • In this assay, red blood cells (RBCs) are incubated with a dye, EMA, and the fluorescence is measured in comparison to a control. Immunology Lab
  • A 10 mL starter culture (LB broth with 50 mg/mL carbenicillin) was inoculated from frozen stock and incubated overnight at 37°C with shaking.
  • Cells were passaged into six-well plates and incubated at either 33°C or 39°C. Cell number was assessed 2, 4, 6, and 8 days after subculture by hemacytometer.
  • For experiments using N-formyl-Met-Leu-Phe (fMLP), PMN were incubated with 20 µL per well of 2.5 mM fMLP (Sigma) at 37°C for 5 min before adding opsonized merozoites to start the reaction. PLoS ONE Alerts: New Articles
  • Sealed worker brood was taken from the experimental colonies and incubated until adult emergence.
  • It's just a low-level bug that takes two or three days to incubate and two or three days to get over it.
  • Egypt; and the eggs of innumerable insects, and the spawn of fish, and of frogs, in this climate are hatched by the vernal warmth: this might be the case of birds in warm climates, in their early state of existence; and experience might have taught them to incubate their eggs, as they became more perfect animals, or removed themselves into colder climates: thus the ostrich is said to sit upon its eggs only in the night in warm situations, and both day and night in colder ones Note IX
  • Workplaces are full of people who incubate pointless envy, who nurse rivalries, who are unembarrassed to exhibit ludicrously vaulting ambition. Times, Sunday Times
  • Embryos were incubated with primary antibody overnight at 4° with gentle agitation.
  • If no acid or gas is formed, the bouillon is in a sugar-free condition; but if acid or gas is present, again make the bouillon in the flask +10, reinoculate with one or other of the above-mentioned bacteria, and incubate; then test again. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
  • Males and females have similar roles during breeding; both help build nests, incubate eggs, feed nestlings and fledglings and remove fecal sacks.
  • Sometimes the salad is kept in plastic bags where bugs incubate under artificial supermarket display lights.
  • For immunocytochemistry, grids were incubated with the antibodies against macrophages.
  • Innovation is running rampant in this whole arena, and new businesses are being incubated at a rate never seen before in the industry.
  • Located close to the main entrance of the zoo, the brooder room will be used to incubate eggs and house the hatched chicks.
  • And yet the state, which includes all the citizens of the state, believes that it can blot out this wisdom of mine in the final dark by means of a rope about my neck and the abruptive jerk of gravitation — this wisdom of mine that was incubated through the millenniums, and that was well hatched ere the farmed fields of Troy were ever pastured by the flocks of nomad shepherds! Chapter 1
  • Females build the nest, incubate eggs, and brood nestlings, but both sexes choose the nest site and feed offspring.
  • The birds returned to their nests and continued to incubate the eggs.
  • The transfection efficiency was influenced by different incubate time between ESCORT - DNA mixture and lung cancer cells.
  • Test strains were inoculated into heart infusion broth and incubated overnight at 37 deg C.
  • Monitise was "incubated" by IT firm Morse and spun out in June 2007 after a placing at 22p, but progress has been slow. Latest financial, market & economic news and analysis | guardian.co.uk
  • On the 2nd December, 1877, I found a nest with three slightly-incubated eggs in a small babool bush which stood in a 'sone' grass-field. The Nests and Eggs of Indian Birds, Volume 1
  • The transfection efficiency was influenced by different incubate time between ESCORT - DNA mixture and lung cancer cells.
  • The brain sections from each hemisphere were incubated in 5 ml 55°C formamide for 24 hr. PLoS ONE Alerts: New Articles
  • Only female hummingbirds are involved in parental care; they must incubate eggs, brood young hatchlings, and feed the chicks as nestlings and fledglings.
  • Thirty embryos per sample were incubated for 2 hr at 25°, frozen in liquid nitrogen, and low molecular weight DNA was isolated as described.
  • Hepatocytes damaged by exposure to galactosamine, thiocetamide, and carbon tetrachloride were incubated with Picrorhiza constituents.
  • In the late 19th century, psychologists termed male pregnancy symptoms couvade, from the French word "couver," meaning "to incubate" or "to brood" like a mother bird. The Plight of the Pregnant Man
  • − strains were isolated and re-streaked onto the same plates, but incubated at RT to induce filament and basidiospore formation typical of diploid PLoS ONE Alerts: New Articles
  • To passivate the flow cell, it was subsequently incubated with 10 mg/ml BSA.
  • Additionally, samples from the flocculent layer and from a semipurified bacterial mat were incubated under controlled oxygen conditions to determine production.
  • The female incubates her large clutch, and both parents tend the hatchlings.
  • Also, biofilms differ between incubation sites because an active biofilm did not develop if slides were incubated in a non-adult habitat, such as a sandy intertidal area, but did develop on slides placed at the adult habitat.
  • Multiplexes were developed using an excess of streptavidin-phycoerythrin solution which was thoroughly mixed into each multiplex and incubated for 1 hour at room temperature. PLoS ONE Alerts: New Articles
  • I do not want to extract, digest, ligate, transform, inoculate or incubate anything. Brimstone and Fire
  • In monogamous species, both the male and female build the nest, incubate eggs, brood young and feed nestlings and fledglings.
  • The slides were incubated for 30 min in a humidified chamber at room temperature.
  • The female bird incubates the eggs for about sixteen days while the male brings food.
  • The female bird incubates the eggs for about sixteen days while the male brings food.
  • The same preparation was readapted in darkness and incubated with an appropriate concentration of wortmannin (dissolved in the medium by a series of dilutions of the stock DMSO solution), and the measurement was repeated.
  • Sections were incubated overnight at 70°C with 1 µg/ml of the digoxigenin-labeled probes, and binding detected by overnight incubation with alkaline phosphatase-conjugated anti-digoxigenin antibody (1: 3500, Roche), which was visualised with NBT (Nitro blue tetrazolium chloride)/BCIP (5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt; 1: 50, Roche) or Fast Red (Roche) for fluorescence. PLoS ONE Alerts: New Articles
  • It adjusts the heat needed to incubate the egg by adding to or subtracting from the amount of compost piled above it.
  • In some fish, shrimp, and reptiles, gender is determined by the temperature at which the egg is incubated.
  • The female bird incubates the eggs for about sixteen days while the male brings food.
  • Deletion suppressors obtained were streaked onto SD medium and incubated at 35.5° for 5 days.
  • The entire IN reaction was followed by immunosorbent assay on avidin-coated plates as described previously Fluorescein-labeled peptides at a final concentration of 10 µM in PBS were incubated with HeLa cells for 1 h at 37°C. PLoS ONE Alerts: New Articles
  • VEM-like material that accumulated in incubation fluid when kidney slices were incubated anaerobically. Robert F. Furchgott - Autobiography
  • Cells were incubated in the presence of phorbol ester myristate to induce macrophage differentiation.
  • Three microliters of sterile H 2 O were added to bring the total reaction volume up to 10 l, and the mixture was incubated overnight at 15°C. Transformation and plating were performed using standard procedures.
  • They incubated sedition and plotted with the Iceni against Rome. MIDNIGHT IS A LONELY PLACE
  • Twenty microliters of each antibody was added to 100 L of blood, and the mixture was incubated at room temperature for 15 minutes.
  • Nutrients were thoroughly mixed into the substrate, water was added to field capacity and the pots were incubated for 10 d in a glasshouse before seeds were planted.
  • Females build the nest, incubate eggs, and brood nestlings, but both sexes choose the nest site and feed offspring.
  • Radial water flow was induced by application of a pressure gradient of - 0.06 MPa. Sections were incubated with monoclonal ABA antibodies and with the secondary Alexa 568 antibodies and stained with aniline blue and toluidine blue.
  • Successfully incubated eggs hatch about two months later.
  • Acid insoluble material was pelleted by centrifugation at 14,000 x g for 10 min and the supernatant was mixed with 1/4 volume 100% trichloroacetic acid and incubated on ice for 1 hr.
  • For flowcytometric analysis, CD3+ were kept on ice and washed extensively with citrated PBS containing 1% FCS. 10 µl of FITC, APC or PE - conjugated antibody was added and incubated on ice for 20 min. 300 µl FACS buffer was added and T cells were pelleted, resuspended in 200 µl buffer. PLoS ONE Alerts: New Articles
  • Briefly, 50 µL organ homogenates were incubated with an equal volume of the Griess reagent (1% sulfanilamide, 0. 1% naphthyl ethylenediamine dihydrochloride, 2. 5% PLoS ONE Alerts: New Articles
  • The mixtures were incubated for another 2 hours, followed by adding different concentrations of hot water extracts to the triplicate culture wells.
  • Malignant cells incubated with nanoshells and exposed to the light source showed signs of irreversible heat damage and cell death, but control cells were left undamaged.
  • The germinated seeds were planted in gravel and further incubated under culture room conditions.
  • The BSE bug incubates for three years.
  • Methods Dual-electrode voltage clamp technique was used to record membrane currents from follicular oocytes, and defolliculated oocytes that had been injected with alien RNA and incubated.
  • Three purified colonies from each isolate were further tested on LB, lactose, or glucose minimal medium and incubated at 30° and 42°.
  • Like the eggs of birds, monotreme eggs are incubated and hatched outside the body of the mother.
  • Sections were incubated overnight at 70°C with 1 µg/ml of the digoxigenin-labeled probes, and binding detected by overnight incubation with alkaline phosphatase-conjugated anti-digoxigenin antibody (1: 3500, Roche), which was visualised with NBT (Nitro blue tetrazolium chloride)/BCIP (5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt; 1: 50, Roche) or Fast Red (Roche) for fluorescence. PLoS ONE Alerts: New Articles
  • Using measurements of isotope exchange rates we were led to conclude that the accumulation of triose-P intermediates in human red cells that were incubated with high levels of orthophoshate was due to equilibrium and not Irwin Rose - Autobiography
  • Control cells consisted of untreated cells, cells incubated with the mannitol excipient and exposed to light or cells exposed to the dye alone.
  • The GA solution was dried in a rotor evaporator and then incubated under vacuum overnight in a reagent flask.
  • As a control, sections were incubated with phosphate buffered saline instead of the primary monoclonal antibodies in the first step.
  • We'll meet a baby ankylosaurus incubated from an abandoned egg; rampaging, short-armed carnotauruses; and a pack of nicoraptors, which are like mangy, feral dogs. Terra Nova Exclusive: On the Set of TV's Hottest New Show
  • The chamber was incubated at 37'C for 1.5 h to allow gelation to occur.
  • To explore the mechanism of invasion, gentamicin protection assays were performed with endometrial cells that had been incubated for 1 h in control medium or in medium with the host cytoskeletal inhibitors cytochalasin D (microfilaments) or colchicine (microtubules) before addition of 10 M.O.I. bacteria. PLoS ONE Alerts: New Articles
  • Cells were passaged 1: 20 and incubated at 33°C in 10% fetal bovine serum / Dulbecco's minimum essential medium.
  • Strain DNY75 expressing either human HSF1 (+HSF1) or an empty vector (+vector) were seeded into microtiter wells and incubated in the presence of HSF1A or DMSO solvent for 4 d and then photographed. PLoS Biology: New Articles
  • Other embryos were incubated directly in the Petri dishes for germination at a range of different temperatures.
  • The plate is incubated at 37° for 16-36 hr, and the titer of the colony-forming units is divided by the titer of the microscopic count to determine the viability.
  • Explants were incubated at different temperatures (°C) after bombardment and analysed for reporter-gene expression.
  • Females built the nest and incubated alone, but both parents fed nestlings and fledglings.
  • Shortly thereafter, the female travels over the ice to the open sea to feed, leaving the male to incubate the egg. About two months later she will return to feed and raise the newly-hatched chick.
  • On the shingle beach, where the burnet rose grows, ringed plovers incubate eggs in shallow scrapes.
  • The hen usually lays two eggs, which she incubates for about 24 days until they hatch.
  • The retinas were postfixed for 30 minutes in the same fixative, washed and incubated in a solution containing 0. 02% NADPH-diaphorase and 0. 04% nitroblue tetrazolium PLoS ONE Alerts: New Articles
  • The eggs are incubated for 15 or 16 days and newly hatched troupials are fed equally by both parents.
  • One of the stocks that tanked was a throwback to ARD called CMGI Inc. – a public company that "incubated" infant dot-coms. Money to Make Things New
  • Only female hummingbirds are involved in parental care; they must incubate eggs, brood young hatchlings, and feed the chicks as nestlings and fledglings.
  • Pairs of test and control samples were incubated either in the darkness or under continuous illumination with white light.
  • If the first-laid eggs begin to hatch days before the last-laid eggs, remaining unhatched eggs will be incubated far less frequently and efficiently, because the female will be off the nest foraging for the already-hatched young.
  • The plate is incubated at 37° for 16-36 hr, and the titer of the colony-forming units is divided by the titer of the microscopic count to determine the viability.
  • The intact nuclei were incubated for 15 min after which they were gently pierced with two fire-polished glass micropipettes.
  • Disco was incubated in underground parties in New York City that were attended largely by African Americans but which were run by Italian Americans. A Renegade History of the United States
  • The plates were incubated at 28°C and observed for antibiosis after 24 hours in case of yeasts and 96 hours in case of moulds.
  • Incubators were set at the temperatures indicated, and in all cases plates at different temperatures were incubated in parallel.
  • When most birds incubate eggs, the females often produce high levels of prolactin, a hormone involved in parental behavior.
  • A preincubated, capacitated sperm suspension was gently added to the freshly ovulated oocytes to give a final motile sperm concentration of 1 x [10.sup.6] / ml.
  • Laid in burrows or under mounds, megapode eggs are incubated by the warmth of the sun, rotting vegetation, or volcanic vents.
  • Apparently hens don't lay well in the presence of cocks, and won't incubate their own eggs.
  • Since the parent duckbills were nearly thirty feet long and weighed about three tons, we assume that the dinosaurs were too large to incubate their eggs by sitting on the nest.
  • The embryos are incubated in the laboratory for an additional two to four days and transferred to the female partner's uterus.
  • Other species, however, simply drop the ootheca off in some hidden corner, where the babies can incubate safely while you beat their mothers to death with a shoe. Boing Boing
  • The malvaceous hosts were incubated at 30°C and symptoms were recorded after a two week period.
  • The time needed for the eggs to incubate is nine or ten days.
  • The virus can incubate for up to ten days after the initial infection.
  • The resulting embryos were incubated in 750 ml Zuger bottles at a constant temperature of 28.2 0.2°C until yolk sac resorption.
  • To explore the mechanism of invasion, gentamicin protection assays were also performed with endometrial cells that had been incubated for 1 h in control medium or in medium with 1 µg/ml cytochalasin D or 5 µg/ml colchicine before addition of 10 M.O.I. bacteria. PLoS ONE Alerts: New Articles
  • The formation of this peak increases with irradiation time when the mitochondria are incubated under control conditions and present high membrane potentials.
  • The inoculated cell cultures were incubated at 35C and observed daily for a week for cytopathic effect.
  • Samples were pre-incubated with various concentrations of chloride salts of the monovalent cations and then assayed using the standard procedure.
  • Sometimes the salad is kept in plastic bags where bugs incubate under artificial supermarket display lights.
  • Lymphocytes were stimulated by phytohemagglutinin and were incubated in humidified incubators at 37°C for a total of 72 h.
  • Cells were then permeablized with saponin and incubated with fluorescent-labeled antibodies, and four-color flow cytometry was performed.
  • The retinas were postfixed for 30 minutes in the same fixative, washed and incubated in a solution containing 0. 02% NADPH-diaphorase and 0. 04% nitroblue tetrazolium PLoS ONE Alerts: New Articles
  • Unless, of course, they belong to the new breed of football fan increasingly incubated by the Premier League, specimens who demand that their club be bought by a spendthrift gazillionaire who thinks that balancing books is some sort of primitive kids 'diversion like skipping ropes or hucklebones. Blackburn's prospective owners arrive with promise of financial prudence
  • This hatchling is just one of hundreds of its kind that have been incubated and raised in captivity for release into North America's wetlands.
  • The sample was incubated at room temperature for 4 weeks and the modifications of the secondary structure were monitored by CD while corresponding samples were mounted for electron microscopy.
  • The time needed for the eggs to incubate is nine or ten days.
  • Dace's noble plan was to steal the eggs from the nest and take them somewhere warmer, where they could incubate safely. TREASON KEEP
  • The male emu incubates the eggs alone and accompanies the chicks for up to eighteen months of age.
  • The homogenate was incubated at room temperature for 5 min with agitation and then supplemented with 6 ml of chloroform.
  • The Hong Kong subsidiary will help bring Japanese affiliates of Hikari Tsushin into Asia and incubate them by providing localization support, he added.
  • Detecting anaerobes and microaerophiles is difficult because these microorganisms must be incubated in a controlled gaseous environment that is either oxygen free or extremely oxygen deficient.
  • The bound microparticle complexes are then incubated with antidetection hapten alkaline phosphatase conjugate, which binds to the detection haptens.
  • This co-operative behaviour, in which one chicken incubates the eggs of others to whom she's probably related, has clear biological advantages.
  • (DWA), incubated for 5 d at 15 C until primary sporidia developed, then transferred to room temperature for analysis of ballistospore (secondary sporidia) discharge. PLoS ONE Alerts: New Articles
  • Both help incubate the 2-4 eggs for 4 weeks, and both help brood and feed once the young hatch.
  • They took their generous severance packages to incubate an entirely different lifestyle.
  • Both male and female kingfishers incubate the eggs, which take 2 to 4 weeks to hatch.
  • Cells were washed with phosphate-buffered saline and incubated with Becton Dickinson cocktails of antibodies conjugated to fluorescein isothiocyanate, phycoerythrin, peridinin chlorophyll protein, or allophycocyanin.
  • Doctors "incubated" the girl and planned on taking her to the Staten WN.com - Articles related to Boston weighs smoke-free public housing
  • More than a dozen large eggs were arrayed around the pool, close enough to incubate, but not so close as to cook the young. RISE OF A MERCHANT PRINCE: BOOK TWO OF THE SERPENTWAR SAGA
  • Shoots with 1-2 trifoliate leaves of 3-week-old seedlings were incubated in microtitre plate wells with assay solution in a growth chamber, 16/8 h day/night regime, 21°C.
  • Negative controls were previously incubated in 10 mM tropolone, a specific inhibitor for PPO activity.
  • Both male and female birds incubate the three to four eggs for about 21 days.
  • Three microliters of sterile H 2 O were added to bring the total reaction volume up to 10 l, and the mixture was incubated overnight at 15°C. Transformation and plating were performed using standard procedures.
  • Cells incubated at the permissive temperature demonstrated the typical ‘hill and valley’ appearance.
  • Dace's noble plan was to steal the eggs from the nest and take them somewhere warmer, where they could incubate safely. TREASON KEEP
  • After washing with a staining buffer, cells were incubated with anti-mouse CD16/32 (Fc block, e-Bioscience) for 30 min on ice to block the FcR non-specific binding and subsequently stained for surface markers with PE-anti-CD4, FITC-anti-CD3ε, PerCy-anti-CD8 or isotype controls for PLoS ONE Alerts: New Articles
  • At home in the far north, they rear their families in great haste while the land enjoys its brief sunny respite; the eggs are incubated and the fledglings weaned in less than a month.
  • Taken in mid-morning, it coated the teeth and then incubated until lunchtime; the lactose fermented into lactic acid.
  • On the 2nd December, 1877, I found a nest with three slightly-incubated eggs in a small babool bush which stood in a 'sone' grass-field. The Nests and Eggs of Indian Birds, Volume 1
  • The female incubates the clutch of eggs, which can vary from 4 to 6, but usually consists of 5 eggs.
  • After canned stewed pork with brown sauce was cleaned, basketed, sterilized and incubated, we succeed in producing good quality stewed pork with brown sauce.
  • The chicks have now been removed from the father - who, in the case of the rhea family, incubates the eggs and cares for the chicks.
  • He incubates the eggs for around 23 days and tends the brood after they hatch.
  • Four, 10 to 15 day old embryonated chicken eggs were each inoculated via dropped chorioallantoic membranes with 0.1 mL of sample and incubated at 37 deg C as described by Senne.
  • For experiments using N-formyl-Met-Leu-Phe (fMLP), PMN were incubated with 20 µL per well of 2.5 mM fMLP (Sigma) at 37°C for 5 min before adding opsonized merozoites to start the reaction. PLoS ONE Alerts: New Articles

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