How To Use Eluate In A Sentence
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The eluate was dialyzed against 50 mM Tris pH 7.9 overnight at 4
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After removal of the methanol of the combined eluates under reduced pressure, the pH of the aqueous fraction was adjusted to 3.0 and partitioned three times against ethyl acetate at pH 3.
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The “iso” form of the amide is recovered by evaporating the chloroform eluate to dryness in vacuo.
L.S.D., R.I.P.
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Then, the eluate was diluted to a final concentration of 0.1% SDS with lysis buffer and immunoprecipitation was repeated with the supernatant with fresh beads and antibody.
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The HPLC instrument consisted of Rheodyne rotary valve which was equipped with a 100 ml loop, in order to collect sufficient eluate from the column for scintillation studies.
Chapter 7
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This elution step was repeated twice and eluates were pooled.
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Aliquots of eluates containing proteins were rapidly frozen in liquid nitrogen and stored at - 80°C for protein determination and enzyme activities.
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Radioactive thiols in the eluate were identified by comparison with the fluorescence peaks of mBBr derivatives and by S-cysteine, S-glutathione and S-sulphate standards.
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The eluate is treated with tartaric acid to form the acid tartrate of d-lysergic acid N,N-diethyl amide which is isolated.
L.S.D., R.I.P.
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Concentrated CKs were eluted from the cartridges with ethanol and the eluates were evaporated to dryness.
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Immunoblots using the anti-His antibody showed that wild type PLN was present in the COP-precipitated eluate however, PLN RΔ14 was not co-immunoprecipitated.
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The column eluate was monitored at 215 nm, and 1 min (1 ml) fractions were collected.
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This causes the smaller molecules to appear in the later fractions of the eluate.
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FOXL2 is sumoylated in vivo in physiological conditions: (D) Immunoprecipitation was done using anti-FOXL2 antibody on α-T31 cell lysate and the eluate analyzed by western blotting using anti-FOXL2 antibody (left panel) or anti-SUMO-1 antibody (right panel).
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