How To Use Cistron In A Sentence
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We find that full function of the endogenous rDNA locus depends not merely on the presence of the rDNA cistrons but also on chromosomal context.
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This idea is expressed by the classic slogan of Beadle: "one gene - one enzyme", or in the more sophisticated but cumbersome terminology of today: "one cistron - one polypeptide chain".
Francis Crick - Nobel Lecture
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Molecular evolution of the rDNA cistron in these plants typically follows the same trajectory.
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Almost all eukaryotes have multiple copies of their nuclear ribosomal RNA cistrons, arranged in a long tandem array.
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The strategy of these authors entailed the introduction of a polycistronic vector encoding the genes c-Myc, Klf4, and SV40LT and selection of stable episomes that provided persistent gene expression to achieve reprogramming followed by the identification of vector-free subclones after several passages in the absence of selection.
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Using a microRNA microarray, developed in collaboration with Scott Hammond at the University of North Carolina, He compared the expression of microRNAs-known as the mir-17-92 polycistron-suspected of playing a role in tumorigenesis in cancerous and normal cell lines.
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Almost all eukaryotes have multiple copies of their nuclear ribosomal RNA cistrons, arranged in a long tandem array.
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They explicitely explain that streptomycin resistance is the result of the organisms singular rRNA cistron, which renders them with one reversible mutation from almost completely resistant/non-resistant.
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The estimate was defined at the scale of cistrons so as to exclude the possibility of recombination.
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Benzer called this unit the ‘cistron’; molecular biologists came to view the cistron as the nucleotide sequence encoding a single polypeptide.
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Stem Cell Cassette (STEMCCA) polycistronic lentiviral vector
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Similarly, a Cre-expressing plasmid or adenovirus was employed to remove three or four copies of a polycistronic vector from the genome of mouse iPSCs
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As I was grappling with some of the more technical passages that contained words like allele, nucleotide, cistron, and mitosis,
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Alternative initiation strategies by ribosomal frameshifting and leaky scanning enable polycistronic translation of the cap-dependent viral transcript.
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(CB) in prostate cancer was carried out using single and bi-cistronic siRNA-expressing constructs.
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….then there is considerable scope to modulate the expression of the cistronic genome without any significant alteration of coding sequences.
It's more than genes, it's networks and systems - The Panda's Thumb
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Notably, these studies demonstrated that somatic cells carrying a single proviral copy of the polycistronic cassette can be reprogrammed to pluripotency, substantially reducing genomic modification of the target cell.
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If we propose that these regulate cistronic activity by their binding of proteins *like* transcription factors and cis/trans factors, *and* their binding of RNA sequences let us regard these too as transcription factors potentially with their own point mutations.
It's more than genes, it's networks and systems - The Panda's Thumb
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We find mir-424 and mir-503 are derived from a polycistronic precursor mir-424-503 that is under repression by the MLL-MLLT3 leukemogenic fusion.
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Among others, many prokaryotic messages are polycistronic, few eukaryotic.
Uncommonly Dense blows common descent. - The Panda's Thumb
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A feasible way to decrease the risk of insertional mutagenesis is to combine the reprogramming factors into a single polycistronic vector by inserting a 'self-cleaving' 2A peptide or an internal ribosome entry site (IRES) sequence between two consecutive open reading frames.
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A number of studies have recently demonstrated the advantage of using polycistronic vectors for iPSC generation.
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