How To Use Chromatography In A Sentence
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The extracts obtained were prefiltered through glass wool and sodium sulfate anhydride and filtered by column chromatography (clean-up performed using sodium sulfate anhydride and Celite 545).
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Detecting by hydrogen flame ionization detector with gas chromatography increased sensitivity and decreased detection limit.
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A method for separation and determination of cantharidin from the culture of medicinal fungi using capillary gas chromatography was developed.
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Gradient elution chromatography was applied to determine the ginsenoside contents in Shengmai injections.
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The samples were subsequently desalted by exclusion chromatography over 5 mL Zebra Desalt spin columns, as recommended by the manufacturer (Pierce).
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Final separation was achieved by fast protein liquid chromatography, followed by dialysis and lyophilization, resulting in a white powder.
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The assay was validated directly by gas chromatography/mass spectrometry.
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Following chromatography, the plate was dried and autoradiographed.
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Some of the most common separation techniques are leaching, flotation, filtration, chromatography, and centrifugal force.
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This revised and expanded edition has two new chapters on capillary electrophoresis and supercritical fluid chromatography.
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Some fractions with low molecular weight have been obtained by silica gel chromatography separation of Pingshuo coal extracts.
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Analysed the concerned conceptions and non comprehensive evaluating reasons by the grey clustering analysis. Got the overall evaluating results in gas chromatography column seperation and evaluation.
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The product was isolated by silica gel column chromatography utilizing a gradient system of increasing polarity.
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Objective To establish a liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the simultaneous analysis of cocaine(COC) and its metabolite benzoylecgonine(BZE) in urine samples.
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Using vacuum headspace distillation they isolated volatiles from 20 kg of raw onions and then used solid phase extraction and preparative capillary gas chromatography to obtain 10g of the key compound.
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The other way is to use gas chromatography/mass spectrometry to measure how much of the offending compounds are in pond water.
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Ultimately, alliinase was purified up to 20.68 times with a recovery of 38.53% by HA chromatography.
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The vitamins were assayed by high-performance liquid chromatography and the trace elements by atomic-absorption spectroscopy.
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Traditionally, these analyses are done with gas chromatography, which is expensive, time-consuming and requires bulky devices and skilled analysts.
Science News / Features, Blog Entries, Column Entries, Issues, News Items and Book Reviews
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The favorable conditions of high performance interaction chromatography(HPHIC) for purifying microsomal P450 in midgut of Helicoverpa armigera were established.
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The degree of sulfonation was ascertained by means of chromatography.
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A method for separation and determination of sulfur compounds in gasoline fraction of vaccum gas oil microreactor products by gas chromatography - atomic emission detector(GC- AED) was established.
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Total lipid extracts were separated into neutral and polar lipid fractions by column chromatography on Florisil 60-100 mesh.
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Chromatography is a technique for analyzing mixtures of gases, liquids, or dissolved substances.
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The quality of lipids was tested by thin layer chromatography.
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Many diagnoses, such as the organic and amino acid disorders are routinely made by the analysis of urine and plasma specimens using gas chromatography/mass spectrometry and amino acid quantitation using ultra-performance liquid chromatography respectively.
Metabolic Disease Lab
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Both blood and urine screening tests use thin layer chromatography to separate the amino acids present.
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The charcoal tubes were desorbed with 1 ml of methylene chloride and analyzed by gas chromatography and mass spectrometry.
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BHPLC-MS analysis was performed with an agilent 1100 series high performance liquid chromatography (HPLC) connected to a micromass LCT TOF MS.
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Using high pressure liquid chromatography, the alkaloids can be purified and crystallized.
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Amino acid analyses were carried out by ion exchange chromatography on sulphonated polystyrene resin linked to divinyl benzene.
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The comparative study was conducted by trait identification, microscopic identification, thin-layer chromatography and ultraviolet spectroscopy.
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E. coli SSADH is also redox regulated, furthermore it reveals that the bacterial SSADH is structurally suited for NADP Recombinant E. coli SSADH was purified as a tetrameric molecule (determined by analytical size-exclusion chromatography; data not shown) which is in agreement with the previous description in the literature.
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If the stationary phase is solid, the separation process is called adsorption chromatography.
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In the experiment, we have found that anion exchange chromatography is essential to isolate mixture.
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When these results were re - investigated using gas chromatography the earlier work was confirmed.
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Total bile acid concentration was calculated as the sum of all gas liquid chromatography detected individual bile acids.
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Hydroxyproline was quantified by reverse-phase high performance liquid chromatography (HPLC) of 7-chloro-4-nitrobenzo-oxao-1,3, - diazole - derived acid hydrolysates; the total left lung collagen was then calculated in milligrams, assuming that lung collagen contains 12. 2% (w/w) hydroxyproline.
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Multi-residue screening of endocrine disrupters chemicals in water samples by stir bar sorptive extraction-liquid desorption-capillary gas chromatography-mass ...
OpEdNews - Quicklink: Experts: Endocrine Disruptor BPA Found in Bottles Worrisome
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Screening for high-oleic acid by any breeding programs was done by measuring fatty acid content of the seeds by using gas chromatography, which is a destructive method.
Clovis News Journal : News
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During the column chromatography of aequorin, we found a trace of protein that showed green fluorescence, which eluted sooner than aequorin.
Osamu Shimomura - Autobiography
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Preparative high-speed counter-current chromatography (HSCCC) was used for the separation of calycosin and formononetin from the ethyl acetate extract of Astragalus membranaceus Bge.
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Wild carrot lectin was isolated and purified by chromatography on CM-cellulose and DEAE-cellulose.
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The methods of studying flow profile of preparative chromatography were summarized.
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Objective:To establish a gas chromatography method for determination of methacrylonitrile in the air in a workplace by solvent desorption.
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We use gas chromatography for moisture determination in a number of grains.
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As a good chemist should, he took up his compound in some solvent and filtered it, so as not to introduce grit and crud into the pumps or the chromatography column.
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Thin layer chromatography or spectrophotometry is used for screening.
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Trypsin was covalently linked with chemical modified granule chitosan and was used to isolate and purify aprotinin from the extract of cattle lungs by affinity chromatography.
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Their method takes just 15 minutes to separate the compounds in crude extracts using high-performance liquid chromatography.
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The citronella oil content of certain candle formulations was determined by headspace gas chromatography - mass spectrometry.
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The mercurial bromide was re-extracted and analyzed by capillary gas chromatography/electron capture detector, using an internal standard for quantification.
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Monoclonal antibody produced by continuous perfusion culture was recovered and purified by expended bed adsorption chromatography.
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Develop and validate cell based activity methods for protein analysis, glycosylation (glycan isoforms & sialic acid) and heterogeneity ( C & N terminal sequencing, peptide mapping and size exclusion chromatography).
News - chicagotribune.com
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Analysed the concerned conceptions and non comprehensive evaluating reasons by the grey clustering analysis. Got the overall evaluating results in gas chromatography column seperation and evaluation.
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Five microlitres of the remaining extract was analysed by thin layer chromatography according to Wollenweber.
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A gas chromatography-mass spectrometry (GC/MS) analytical method for three anorectics drugs, fenfluramine, diethylpropion and mazindol, illegally adulterated in slimming foods has been developed.
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Our sample-preparation methods split the fatty acids from the alcohol components of glyceryl and steryl esters to form fatty-acid methyl esters, which are more amenable to analysis through gas chromatography-mass spectrometry.
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At present, modern technology has been adopted and includes: microscope appraisal, thin layer chromatogram, thin colorimeter, thin luminosity chromatogram, the combination of the chromatography and gas chromatography-mass spectrometer, and the highly effective liquid chromatography.
Chapter 8
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There is no mention of environmentally important techniques as gas chromatography, mass spectrometry, atomic absorption spectrometry, etc.
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ABSTRACT Object: To develop a gas chromatography method for the determination of residual organic solvents in lomefloxacin.
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Also it can be matched with all kinds of gas chromatography instruments in home and abroad, and meet with a favorable reception in this industry.
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Finally in 1945, scientists isolated promethium from uranium fission products purified by ion-exchange chromatography.
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These include metabolite measurements for plasma, urine and cerebrospinal fluid amino acids using ion exchange chromatography, urine organic acids using gas chromatography/mass spectrometry, and total and free carnitine levels and quantitative acylcarnitine profiles using electrospray tandem mass spectrometry.
Laboratory Rotation
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All ignitable liquids were confirmed by gas chromatography and mass spectroscopy, according to the report.
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A high performance liquid chromatography - mass spectrometric ( HPLC - MS ) method for microdetection of carbendazim in Dendrobium candidum Wall.
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The volatile profile of fruits determined by gas chromatography and mass spectroscopy is complex, including many alcohols, aldehydes and esters.
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Therefore, a high-performance liquid chromatography method was developed to assay sulfadimidine.
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Steam distillation or chromatography of wood and wood residues can release terpenes.
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Amino acid analyses were carried out by ion exchange chromatography on sulphonated polystyrene resin linked to divinyl benzene.
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Total bile acid concentration was calculated as the sum of all gas liquid chromatography detected individual bile acids.
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Gas chromatography method is simple, rapid and accurate. It can be applied to determine sodium cyclamate in milk beverages.
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Recently, simplified ion-exchange column chromatography methods (commonly termed a mini-prep) have largely supplanted the cumbersome and time-consuming centrifugation protocol to yield large quantities of endotoxin-free plasmid DNA in a relatively short period of time.
Archive 2005-10-01
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Fatty acid methyl esters were analyzed by gas chromatography as described above.
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The charcoal tubes were desorbed with 1 ml of methylene chloride and analyzed by gas chromatography and mass spectrometry.
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In this work we have measured protein interactions using self-interaction chromatography, in which protein is immobilized on chromatographic particles and the retention of the same protein is measured in isocratic elution.
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Preparative high - speed counter-current chromatography ( HSCCC ) was used the isolation and purification of solanesol from potato leaves.
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I did a gas chromatography mass spec to check for DDT, but that came back negative, so I isolated some dinoflagellate DNA, and ran a gel.
MINUTES TO BURN
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But that was using immunoassay and thin-layer chromatography.
Harvest
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Hydroxyproline was quantified by reverse-phase high performance liquid chromatography (HPLC) of 7-chloro-4-nitrobenzo-oxao-1,3, - diazole - derived acid hydrolysates; the total left lung collagen was then calculated in milligrams, assuming that lung collagen contains 12. 2% (w/w) hydroxyproline.
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A gas chromatography method recommended by NIOSH for trimellitic anhydride should be adaptable to organic anhydrides that can be volatilized without decomposition.
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We measured the circumference of the waist and hips, and we analysed subcutaneous abdominal adipose tissue by gas chromatography for fatty acids.
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Principle The test card is based on immunochromatography of double-antibody sandwich.
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In both types of chromatography, portions of each component remain dissolved in the mobile phase.
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The hepatopancreas index(HI), lipid content(LC) and fatty acid composition in Eriocheir sinensis before and after the precocity, were compared by gravimetry and gas chromatography.
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In addition , the hydrogen detection by gas chromatography brought less molestation, wastage and time consumption.
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Elastase activity was determined in a spectrophotometric assay using elastin-Congo red (Sigma) as a substrate, as previously described Liquid chromatography/electron spray ionization mass spectrometry (LC-ESI-MS) data on pure rhamnolipid was used to produce a standard curve for rhamnolipid B (concentration vs. total ionization current (TIC)).
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Have a thorough understanding of laboratory procedures and laboratory skills, the practices, principles, and theory of specific disciplines such as chromatography, raw material analysis, laboratory instrumentation, etc (essential)
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A method was developed for the simultaneous determining of Clenbuterol, Salbutamol, Terbutaline and Fenoterol residues in pork tissues using gas chromatography-mass spectrometry(GC-MS).
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I did a gas chromatography mass spec to check for DDT, but that came back negative, so I isolated some dinoflagellate DNA, and ran a gel.
MINUTES TO BURN
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Deoxycytidine was separated from cytidine using thin-layer chromatography.
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To gain more insight into the salt-dependent behavior of SecB, we studied the protein by DLS, size exclusion chromatography, analytical ultracentrifugation, and small angle neutron scattering.
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Total bile acid concentration was calculated as the sum of all gas liquid chromatography detected individual bile acids.
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Reverse phase high-performance liquid chromatography(RP-HPLC) was applied to determine the S-adenosyl-l-methionine(SAM) in erythromycin fermentation course.
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This has important applications in protein chromatography, immunoassays for medical diagnostic tests, development of biosensors, biotechnology, and biocatalysis.
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Fatty acid methyl esters were analyzed by gas chromatography as described above.
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The lipid was found to migrate as a single spot under thin layer chromatography.
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All ignitable liquids were confirmed by gas chromatography and mass spectroscopy, according to the report.
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The platinated oligonucleotides were repurified by ion-exchange fast protein liquid chromatography.
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Like flow cytometry, gas chromatography and gas chromatography-mass spectrometry, the technologies used to detect the presence of the chemicals used in the production of plastic, are well established.
The Explainer: Plastics
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The dichloromethane extract of S. crispus was chromatographed on silica gel by flash column chromatography.
BioMed Central - Latest articles
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Partial dehydration to about 4% water by weight produces the commercial silica gel product, a highly porous material often used as a drying agent or adsorbent for chromatography.
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Metabolites of gylcolysis and the citrate cycle were measured by ion chromatography coupled to mass spectrometry (IC-MS).
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Protein kinase C was partially purified from rat liver by homogenization, ammonium sulfate fractionation and DEAE-cellulose chromatography.
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The residue was redissolved in 0.5 ml of hexane and analysed by gas-liquid chromatography.
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This isocratic system consists of a high pressure solvent delivery pump, an UV/VIS detector, a manual injection valve, and a solvent tray, a column and a Chromatography Workstation.
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Amino acid analyses were carried out by ion exchange chromatography on sulphonated polystyrene resin linked to divinyl benzene.
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Ultimately, alliinase was purified up to 20.68 times with a recovery of 38.53% by HA chromatography.
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The quantification of these compounds was performed by chromatography before and after laser irradiation.
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Quantitative analysis, by means of gas chromatography-mass spectrometry, identified guaiacol, 4-methylguaiacol, 4-ethylguaiacol, 4-ethylphenol, eugenol, and furfural in each of the wines made from smoked grapes.
When Smoke Gets in Your Grapes
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Chromatography was first developed more than a century ago and solid state gas chromatography is more than 60 years old.
The Explainer: Plastics
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There is no mention of environmentally important techniques as gas chromatography, mass spectrometry, atomic absorption spectrometry, etc.
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Other methods of molecular weight determination include gel permeation chromatography, end-group analysis, and osmometry.
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This paper describes the device and the fundamental procedures used in the method of fast measuring diffusible hydrogen within weld metal by a gas chromatography.
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The IBP team has applied gas chromatography and olfactometry to the problem.
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The separation and final purification of the two enzymes was achieved by anion exchange chromatography.
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Fatty acid methyl esters were analyzed by gas chromatography as described above.
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Objective To prepare a kit for detecting apoprotein(Apo) A1 and B. Methods Dextran sulfate precipitation, ultracentrifugation, chromatography, and so on.
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- C incorporates technological advances such as nanofiltration, a virus exclusion technology, and cation exchange chromatography, an additional purification step, "said Steve Petteway, Ph. D., executive vice president, Research and Development.
THE MEDICAL NEWS
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Biotin and avidin (or streptavidin) bind non-covalently with a higher binding affinity than most antigen-antibody interactions. 1, 2 This very tight binding makes labeling proteins with biotin a useful tool for applications such as affinity chromatography and immunoanalytical methods.
PR.com Press Releases
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In adsorption chromatography, solute molecules of the mobile phase undergo many successive adsorptions and desorptions.
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The procedure for the isolation of PHU from freshly collected male urine included filtration, ultrafiltration and reverse-phase chromatography.
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Sensitivity can be readily increased by derivatizing with a UV-absorbing or fluorescent species and separation by micellar electrokinetic capillary chromatography.
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The variation of the roasted green tea aroma during tea processing was studied by gas chromatography and mass spectrograph (GC/MS).
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The principle and the specific method of cyclamate determination in wine by capillary gas chromatography are introduced.
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This revised and expanded edition has two new chapters on capillary electrophoresis and supercritical fluid chromatography.
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The favorable conditions of high performance interaction chromatography(HPHIC) for purifying microsomal P450 in midgut of Helicoverpa armigera were established.
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The charcoal tubes were desorbed with 1 ml of methylene chloride and analyzed by gas chromatography and mass spectrometry.
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Even if the common method of forming diastereomeric salts with a chiral acid was inadequate for resolution, the skilled person would employ the chiral chromatography method known to him and established pre-priority (referring to I.W. W.iner, Trends in Analytical Chemistry 6 (1987) 125-134 for commercially available chiral stationary phases which the skilled person would use at the priority date).
The IPKat - supporting prudent IP use
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Finally, the Fab fragments are isolated by an ion exchange and affinity chromatography columns. 4 Crofab is indicated for treatments of minimal to moderate cases of North American crotalid envenomation because the current studies were only documented for these conditions. 4
Crotalidae Polyvalent Immune Fab (Ovine)
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They analyze a wide variety of chemical compounds, using testing methods such as chromatography or spectroscopy.
BioSpace.com Featured News and Stories
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Under the leadership of Tiselius this institute has contributed to the development and improvement of a number of useful methods in biochemistry, such as electrophoresis, chromatography, phase partition, gel filtration, etc.
Arne Tiselius - Biography
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Five microlitres of the remaining extract was analysed by thin layer chromatography according to Wollenweber.
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Objective: To establish a gas chromatography method for determination of methoxychlor in workplace air.
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Essentially, chromatography is a filtering process that allows distinct compounds or molecules to be separated by molecular size and weight.
Richard Kuhn and the Chemical Institute: Double Bonds and Biological Mechanisms
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The procedure for the preparation of CDA-II from male urine includes filtration, ultrafiltration and reverse-phase chromatography.
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This process of separating molecules by size and weight is called chromatography ” so named because it was first used in the laboratory to separate colored dyes.
In the Primordial Soup
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In comparison to gas chromatography and mass spectroscopy, the use of an electronic nose for detection of lung cancer offers several potential advantages, including ease of administration of the test and portability.
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High-fructose corn syrup is made through a process called chromatography, or a distillation of the fructose, in which it is exposed to a series of enzymes to convert cornstarch to 90 percent fructose.
The Daily Barometer
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After the chromatography was complete, dried plates were sprayed with a solution of 2% ninhydrin in acetone (w/v), followed by heating.
