How To Use Centrifugation In A Sentence
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There are different kinds of lipoproteins and they are classified on the basis of their density as determined by ultracentrifugation: Low
Physiology or Medicine 1985 - Press Release
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Soluble and insoluble fractions were separated by centrifugation in glass centrifuge tubes.
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The glycerin (another name of for glycerol) is removed from the system, and soap is isolated by using centrifugation and neutralization processes.
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The cell suspension was collected in an Eppendorf tube and sedimented by gentle centrifugation.
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The large and small are measured by electrophoresis whereas VLDL is measured by ultracentrifugation.
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The present study indicates that DNA resolubilization at high concentrations of spermine and spermidine is explained by formation of small particles of condensed DNA that cannot be precipitated by centrifugation.
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I measured the molecular weight of the purified H3/H4 preparation by equilibrium ultracentrifugation, while
Roger D. Kornberg - Autobiography
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The dialysate was clarified by centrifugation and stored at - 80C. Protein concentration of the HSV - 2 Rl preparation was measured by the method of Bradford using y-globulin as a standard.
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The crystals were harvested by centrifugation and redissolved in water.
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Such nuclear suspension was sedimented by gentle centrifugation, the enzyme solution was decanted, and the nuclear pellet was resuspended in 1 x PBS buffer.
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To gain more insight into the salt-dependent behavior of SecB, we studied the protein by DLS, size exclusion chromatography, analytical ultracentrifugation, and small angle neutron scattering.
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The bacterial culture is concentrated by centrifugation and disrupted by lysis with an alkaline detergent solution containing enzymes to degrade contaminating RNA.
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Insoluble debris was removed by centrifugation for 5 min at 14000 rpm in a microcentrifuge.
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When performing microarrays to evaluate leukemias, normal and leukemic cells found in blood or bone marrow are first separated by density gradient centrifugation.
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Nuclei are then separated from other cell components by differential centrifugation.
The Scientist
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After centrifugation , broth culture precipitates were smeared and stained with fuchsin to observe the morphology change in thallus under optical microscope.
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“Was the RNA degraded in the centrifugation step?”
The Emperor of All Maladies
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Acid insoluble material was pelleted by centrifugation at 14,000 x g for 10 min and the supernatant was mixed with 1/4 volume 100% trichloroacetic acid and incubated on ice for 1 hr.
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The washing and centrifugation steps were repeated three times to remove all unabsorbed solutes.
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The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer).
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Cell debris was removed by centrifugation, and the supernatant was fractionated by ammonium sulfate precipitation.
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Recently, simplified ion-exchange column chromatography methods (commonly termed a mini-prep) have largely supplanted the cumbersome and time-consuming centrifugation protocol to yield large quantities of endotoxin-free plasmid DNA in a relatively short period of time.
Archive 2005-10-01
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Cells were separated from the culture medium by centrifugation at 100 g for 1 min at 4°C.
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If a passenger candidate develops electrocardiographic abnormalities during centrifugation, the centrifuge can be stopped and, most likely, the irregular heart rhythm will revert to normal beating.
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Insoluble material was then removed by centrifugation.
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This is probably the most accurate method but requires centrifugation of the sample, analysis of the separated plasma, and use of a moderately complex equation.
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These treatments also useful for centrifugation and sedimentation.
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I'm in the midst of a 3.5 hour centrifugation, so I figured I might as well do follow up on her tag.
Random Things
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In 2000, the organism was cultured in human fibroblast cell line by centrifugation shell vial technique.
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The total lysate separated into 4 different fractions whereas only the one with the lowest density was detected when the supernatant recovered after ultracentrifugation was loaded onto the sucrose gradient.
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Cell debris was removed by centrifugation, and the supernatant was fractionated by ammonium sulfate precipitation.
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Objective To prepare a kit for detecting apoprotein(Apo) A1 and B. Methods Dextran sulfate precipitation, ultracentrifugation, chromatography, and so on.
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Two novel chlorophyll (Chl) - containing supercomplexes (F4 and F5) in addition to the photosystem (PS) I trimers (F3) were observed by sucrose gradient ultracentrifugation in
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DNA was ex - tracted from a sample of the original N15-labeled cells and subjected to ultracentrifugation in a cesium chlo - ride density gradient, which differentiates molecules by weight.
GENETIC CONTINUITY
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The aqueous phase was collected by centrifugation, dried under vacuum, and redissolved in 0.7 ml of water.
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Alcohol (isopropanol) precipitation concentrates the eluted plasmid DNA, which is collected by centrifugation, washed, and redissolved in buffer.
Archive 2005-10-01
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Whole lysates of mouse organs (brain, liver, kidney, spleen, heart, and lungs), embryonic day 12 embryos, and cultured fibroblasts, clarified by high-speed centrifugation, were preabsorbed on the column with coupled preimmune serum.
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Alcohol (isopropanol) precipitation concentrates the eluted plasmid DNA, which is collected by centrifugation, washed, and redissolved in buffer.
Archive 2005-10-01
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After an initial 10-min centrifugation to pellet cell debris, the samples were processed according to the manufacturer's recommendations.
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Anything from microdissection of individual cells to gradient centrifugation of enzymatically digested tissue might be necessary.
The Scientist
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The isolation of such nuclei has traditionally been achieved through detergent-based cell lysis followed by fractionation via differential centrifugation.
The Scientist
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One such method is ultracentrifugation, developed by The Svedberg from
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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After cooling, the flocculent precipitate was removed by centrifugation at 4 [degrees] C, 1,000 g for 10 min.
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The sarcosine insoluble fractions were removed by centrifugation at 100,000 g for 1 h in an ultracentrifuge, washed once with PBS, pH 7.4 and used as outer membrane protein fractions.
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After removing the cell debris by centrifugation, supernatant was layered on a 5.7 M CsCl / 0.01 M EDTA solution in an ultracentrifuge tube and centrifuged in a swinging bucket rotor at 40,000 rpm for 16 hr.
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After ultracentrifugation, the free peptide concentration was determined fluorometrically.
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The supernatant from the first centrifugation was centrifuged at 10,000 × g for 10 min.
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The homogenate was then transferred to a Dounce homogenizer and further homogenized with 30 strokes of the pestle, and then subjected to centrifugation at 1,500
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The following day, cells were harvested by centrifugation, resuspended in buffer (20 mM Tris-HCl, pH 8.0; 100 mM NaCl), and disrupted by sonication.
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Cell debris was pelleted by centrifugation and supernatant concentrated by lyophilization to one tenth of the initial volume.
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Cell debris was pelleted by centrifugation and the supernatant was tested for protein induction.
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After centrifugation, the cell pellet was processed for microgel electrophoresis to determine sensitivity to mutagens.
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Total leukocyte count was determined with a hemacytometer, and differential count by cytocentrifugation of 300 µl of bronchoalveolar lavage fluid at 450×g for 5 min, followed by Wright-Giemsa staining.
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Soluble and insoluble fractions were separated by centrifugation at 8 000 g for 30 min at the extraction temperature.
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The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C.
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In the current context, for illustrating the behavior of the size distribution, we considered the data from a mixture of two different elution fractions after sucrosegradient ultracentrifugation.
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Unbound phages were separated from phages bound to antibodies in normal plasma by centrifugation.
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Lysates were cleared by centrifugation for 20 minutes at 15,000 rpm in a microcentrifuge and the supernatants were assayed for protein concentration by Bradford assay using BSA as a standard.
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Cellular components were sedimented by centrifugation at 4 [degrees] C, 500 xg for 10 min.
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Filtered cells were washed by centrifugation, twice with acetone and twice with diethyl ether.
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Motile sperm were isolated by Percoll centrifugation, the total RNA extracted and verified using sperm-specific RT-PCR.
Urology Division Research
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Anything from microdissection of individual cells to gradient centrifugation of enzymatically digested tissue might be necessary.
The Scientist
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The insoluble potassium perchlorate was removed by centrifugation at 500 g for 3 min.
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Unbound phages were separated from phages bound to antibodies in normal plasma by centrifugation.
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This was shown in the earlier analytical ultracentrifugation analyses comparing DNA content from thermally ruptured phage (when all of the DNA comes out) with that from samples treated with LamB with and without PEG.
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After mixing and centrifugation, aliquots of the acetic acid phase were injected onto a Chromatographic system for quantitation of catecholamines.
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Pollen was germinated in vitro in liquid medium for 10 min and harvested by centrifugation.
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Pollen was sedimented by centrifugation at 100 g for 2 min and the excess sucrose drawn off by pipette.
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From these preparations peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque centrifugation and two PBS washes From the PBMC, erythroid progenitor cells (EPC) were generated via culture in an expansion medium containing erythropoietic growth factors
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Among these methods were ultracentrifugation, X-ray crystallography, electron microscopy, electrophoresis, macromolecular sequencing, and radioactive tracing.
Gene
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For many years, the method of choice for supercoiled plasmid and virus DNA purification was cesium chloride density gradient centrifugation in the presence of an intercalation agent (such as ethidium bromide or propidium iodide).
Archive 2005-10-01
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This complex was sedimented by centrifugation at 10 000 g for 2 min.
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Sputum cells were sedimented by centrifugation at 400 × g for 10 minutes.
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Debris and insoluble material were pelleted by centrifugation at 12,000 g.
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The optimum conditions was obtained. After centrifugation, pressing, mucor inoculation, dehydration and flavor adjustment, the flavor cheese was obtained, which was of good color, flavor and smell.
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Supernatants were subjected to ultracentrifugation in a Beckman SW28 rotor at
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The volume of apoplastic fluid obtained by centrifugation increased gradually when the centrifugal forces increased.
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The samples were subject to two cycles of freezing/thawing and low molecular weight thiols were recovered in the supernatants after centrifugation at 12 000 g, 10 min at room temperature.
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One such method is ultracentrifugation, developed by The Svedberg from
The Nobel Prize in Chemistry: The Development of Modern Chemistry
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The mixture was pelleted by centrifugation at 13,000g for 8 min and the ethanol removed.
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These authors, by using differential and density-gradient centrifugation techniques, achieved the differentiation of the microbody fraction from lysosomes, microsomes, and mitochondria.
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Fluorescence experiments after centrifugation confirmed that there were no vesicles in the supernatant.
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After the respective diffusion time, sporopollenin capsules were sedimented by centrifugation.
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The glycerin (another name of for glycerol) is removed from the system, and soap is isolated by using centrifugation and neutralization processes.
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The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above.
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For many years, the method of choice for supercoiled plasmid and virus DNA purification was cesium chloride density gradient centrifugation in the presence of an intercalation agent (such as ethidium bromide or propidium iodide).
Archive 2005-10-01
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For the routine measurements of Chl concentration, the cells were sedimented by centrifugation and extracted with 100% methanol.
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The paper of Zerhouni et al JOURNAL OF VIROLOGY, Feb. 2004, p. 1243-1255 explains in more detail how all this is done and looking at their results, I would say that the 6 points mentioned in the VirusMyth Award website are pretty much met, except that nowadays there are other faster and more reliable techniques to isolate viruses other than ultracentrifugation.
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The rest of the pollen mixture was pelleted by centrifugation.
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Alcohol (isopropanol) precipitation concentrates the eluted plasmid DNA, which is collected by centrifugation, washed, and redissolved in buffer.
Archive 2005-10-01
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IsiA-PSI trimers by sucrose gradient ultracentrifugation.
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