How To Use Endonuclease In A Sentence

  • Hamilton Smith telling me about the restriction endonuclease he had discovered in Hemophilus influenzae aroused my interest in the possibility of using restriction enzymes to dissect the genomes of DNA tumor viruses. Daniel Nathans - Autobiography
  • The endonuclease digestion map of pWTA was consistent with the fragment length in anticipation.
  • Endonuclease-sensitive regions of human spermatozoal chromatin are highly enriched in promoter and CTCF binding sequences is to be published in the forthcoming issue of Genome Research. Innovations-report
  • In most cell types DNA cleavage occurs after irreversible activation of endonucleases.
  • Double-stranded RNA is cut into short pieces (siRNA) by the endonuclease Dicer. Advanced Information: The 2006 Nobel Prize in Physiology or Medicine
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  • These blocked forks could potentially be processed into DNA breaks by an endonuclease.
  • Let us remember that they are concerned with a DNA endonuclease, so I had some experience dealing with an enzyme that cut nucleic acids.
  • Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
  • Total DNA was prepared from leaves of barley after 7 days of germination and restricted with appropriate endonucleases.
  • After a restriction endonuclease cleavage, two expected smaller fragments were observed.
  • Subsequently, it has been shown that RISC contains at least one member of the argonaute protein family, which is likely to act as an endonuclease and cut the mRNA (nowadays often referred to as the Slicer function). Advanced Information: The 2006 Nobel Prize in Physiology or Medicine
  • OncoMouse (cancer mouse) for research, through genetic redesign. restriction endonucleases, which are able to cut DNA at specific sites. Citizendium, the Citizens' Compendium - Recent changes [en]
  • Church, “The cleavage site of the restriction endonuclease Ava II,” Nucleic Acids Research 5 1978, 2313–2319. The $1,000 Genome
  • Restriction map A map of a segment of DNA showing the cleavage sites of restriction endonucleases and their physical distance apart, usually measured in base pairs.
  • This study shows that in a competition between the two enzymes, DNA ligase trumps the flap endonuclease in capturing bubbles and creating expanded tracts.
  • Meselson's laboratory at Harvard University to study a DNA endonuclease involved in the replication and recombination of T4 Sidney Altman - Autobiography
  • Restriction map A map of a segment of DNA showing the cleavage sites of restriction endonucleases and their physical distance apart, usually measured in base pairs.
  • The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.
  • Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
  • This was an exciting period in the molecular biology of adenovirus with the discoveries (a) that only one specific fragment of the genome, the E1 region, was responsible for oncogenic transformation; (b) that restriction endonuclease length polymorphism could be utilized to generate genetic maps; (c) the mapping of specific genes on the viral genome; and (d) generation of a viral map of sequences expressed as stable RNAs. Phillip A. Sharp - Autobiography
  • Primers and endonucleases are used in the cloning of the various constructs.
  • Secondly, the preamplified DNA fragments were digested by a restriction endonuclease to form sticky ends, which were then ligated to a designed DNA adapter by ligase.
  • The second involves the joining of DNA ends through the pairing of short complementary sequences, as happens with complementary overhanging DNA ends produced by endonucleases.
  • The pdb-codes of the proteins are as follows: endonuclease III - 2ABK, dihydrodipicolinate synthase - 1DHP, blue copper oxydase CuoE - 1KV7, threonine dehydratase - 1TDJ, penicillin amidase - 1PNK. PLoS ONE Alerts: New Articles
  • CP4-EPSPS gene was amplified from 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of PCR products.
  • Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification.
  • Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.
  • Ulf, I, and others generated the first restriction endonuclease maps of this virus, and Dr.S. Jane Flint and I began to analyze the regions of the genome expressed as mRNAs in both productively infected cells and in adenovirus transformed cells. Phillip A. Sharp - Autobiography
  • The core technology in TILLING is screening mutant library by using single-strand endonuclease.
  • Cleavage of DNA by R1 restriction endonuclease generates cohesive ends," Proc. Asilomar and Recombinant DNA
  • After all, in nature we can and do observe bacteria using restriction endonucleases and ligases to cut DNA.
  • The chemistry of water activated by a magnesium ion is central to the function of the DNA repair proteins, apurinic/apyrimidic endonuclease 1 (Ape1) and polymerase A (Pol A). Signs of the Times
  • AP endonuclease recognizes this site and nicks the damaged DNA on the 5 'side (upstream) of the AP site creating a free 3'-OH. Another Protozoan and Front-Loading
  • Samples were digested with the appropriate restriction endonuclease and were size fractionated by electrophoresis through 0.9% agarose gels.

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